NiCoMnO<sub>4</sub>: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition

Qi, Xuewei; Chen, Long; Zhang, Chaoqun; Xu, Xinyuan; Zhang, Yiding; Bai, Yu; Liu, Huwei

doi:10.1021/acsami.6b04280

Cited by 24 publications

(14 citation statements)

References 47 publications

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“…Recombinant proteins with a polyhistidine tag can be efficiently purified using their affinity to metal ions like Ni 2+ . Although immobilized metal affinity chromatography (IMAC) and metal oxide affinity chromatography (MOAC) are standardly used for their purification, several magnetic materials exploiting this powerful affinity were reported [ 184 , 185 ]. Similar mechanism is used also for separation of phosphorylated protein/peptides [ 186 , 187 ].…”

Section: Magnetic Nanoparticles In the Real Worldmentioning

confidence: 99%

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

et al. 2017

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The increasing number of scientific publications focusing on magnetic materials indicates growing interest in the broader scientific community. Substantial progress was made in the synthesis of magnetic materials of desired size, morphology, chemical composition, and surface chemistry. Physical and chemical stability of magnetic materials is acquired by the coating. Moreover, surface layers of polymers, silica, biomolecules, etc. can be designed to obtain affinity to target molecules. The combination of the ability to respond to the external magnetic field and the rich possibilities of coatings makes magnetic materials universal tool for magnetic separations of small molecules, biomolecules and cells. In the biomedical field, magnetic particles and magnetic composites are utilized as the drug carriers, as contrast agents for magnetic resonance imaging (MRI), and in magnetic hyperthermia. However, the multifunctional magnetic particles enabling the diagnosis and therapy at the same time are emerging. The presented review article summarizes the findings regarding the design and synthesis of magnetic materials focused on biomedical applications. We highlight the utilization of magnetic materials in separation/preconcentration of various molecules and cells, and their use in diagnosis and therapy.

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Section: Magnetic Nanoparticles In the Real Worldmentioning

confidence: 99%

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

et al. 2017

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“…[ 180 ] Furthermore, protein tags can be added to the protein of interest to promote metal ion affinity, if not previously present. [ 181 ] The use of nanomaterials and PTM targeting is being applied to a range of central nervous system (CNS) diseases, including dysregulated kinases in Parkinson's disease (e.g., LRRK2). [ 182 ]…”

Section: Nanomaterial‐enhanced Mass Spectrometry In Drug Discovery and Developmentmentioning

confidence: 99%

Engineering Micro–Nanomaterials for Biomedical Translation

Chen

Alba

Tieu

et al. 2021

Advanced NanoBiomed Research

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Engineered nano–bio interfaces–driven by vertical micro/nanoneedles, nanoparticles, organ‐on‐chip devices, and a diversity of nanosubstrates for mass spectroscopy imaging–are spurring scientific and technological progress, from fundamental to transnational biomedical research. Each class has its own characteristic features, which is critical for their translational uptake, but they broadly share the same range of functionality and applicability at the forefront of modern research and medicine. The review provides insights into unique attributes of microneedle technology and its ability for efficient transdermal transport of therapeutic compounds. The uses of nanoneedle technology in precise manipulation of increasingly complex cellular processes at the cell–material interface and their potential for major improvements for many fundamental research applications and ex vivo cell‐based therapies are highlighted. A snapshot in the use of food and drug administration (FDA)‐approved nanoparticle therapeutics and their applications in nanomedicine is provided. The achievements in organ‐on‐chip technology, particularly at the preclinical stage, and its potential to efficiently screen diverse types of therapeutics are covered. The final section is dedicated to the use of nanomaterial‐enhanced mass spectrometry in drug discovery and imaging. Overall, this review aims to highlight those main rules in the design of bio–nano interfaces that have successfully achieved translation into the market.

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“…Min et al constructed a ternary nanomaterial by introducing ceria into magnetic titanoniobate transition metal oxide via the electrostatic assembly approach (dubbed MCTiNbNS), which realized the exhaustive identification of phosphopeptides and accurate count of related phosphorylated sites when using skim milk and human serum as test samples . Moreover, NiCoMnO 4 affinity probe was prepared for the purification of His‐tagged proteins and the capture of phosphopeptides by adjusting buffer pH . Multifunctional CuFeMnO 4 nanosphere was prepared for the capture of phosphopeptides and low‐abundance peptides .…”

Section: Nanomaterials In Phosphoproteomicsmentioning

confidence: 99%

Nanomaterials in Proteomics

Sun

Shen

et al. 2019

Adv Funct Materials

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For a deep understanding of the human proteome, the first crucial step is always the effective separation of targeted proteins/peptides from complex samples. In mass spectrometry‐based proteomics, nanomaterials have emerged as a highly effective means to separate targeted proteins/peptides and increase their relative abundance, which is beneficial to conduct mass spectrometric analysis. In particular, nanomaterials with different specific functionalities have received great attention in ordinary proteomics, phosphoproteomics, and glycoproteomics, for which the easily recognizable characteristics of these proteomes take the primary responsibility, such as the hydrophobicity, phosphate groups, cis–diol structure, and hydrophilicity. This review mainly focuses on the recent design and preparation of nanomaterials with specific recognition ability, as well as their application in the aforementioned three types of proteomics.

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NiCoMnO₄: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition

Cited by 24 publications

References 47 publications

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

Engineering Micro–Nanomaterials for Biomedical Translation

Nanomaterials in Proteomics

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NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition

Cited by 24 publications

References 47 publications

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

Magnetic Nanoparticles: From Design and Synthesis to Real World Applications

Engineering Micro–Nanomaterials for Biomedical Translation

Nanomaterials in Proteomics

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Product

Resources

About

NiCoMnO₄: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition