Nibea coibor growth hormone gene: Its phylogenetic significance, microsatellite variation and expression analysis

Zhang, Dianchang; Shao, Yanqing; Jiang, Shigui; Li, Jianzhu; Xu, Xinping

doi:10.1016/j.ygcen.2009.03.030

Cited by 18 publications

(43 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2). The μGH and mGH loci were both selected because (i) previous genetic studies in Perciformes used an homologous locus to μGH (Almuly et al 2005(Almuly et al , 2008Zhang et al 2009;Chaoui et al 2009), and (ii) complex interactions were reported between this SSR and another intronic minisatellite in seabream, Sparus aurata (Almuly et al 2000(Almuly et al , 2008. Genetic profiles of the three wild and the two experimental (hatchery-derived) samples are shown in Table 1.…”

Section: Genetic Profiles Of Populationsmentioning

confidence: 99%

“…Genotypephenotype relationships that implicate candidate genes are still scarce among fish species. Nevertheless, they are becoming more frequently documented (Pradet-Balade et al 1998;Schulte et al 2000;Streelman and Kocher 2002;Tao and Boulding 2003;Almuly et al 2005Almuly et al , 2008Fromme et al 2009;Li et al 2009;Zhang et al 2009;Blel et al 2010). As noted by Nielsen et al (2009a), the candidate gene approach necessitates the choice of the "right" candidates (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…The involvement of GH and IGF-1 in somatic growth is also clearly established (the GH-IGF-1 system; review in Reinecke 2010), making these genes especially important for cultured species (De-Santis and Jerry 2007). A functional role for genetic variability in influencing levels of gene expression and further growth has only been reported for markers associated with GH (Almuly et al 2005(Almuly et al , 2008Zhang et al 2009) and IGF-1 ) in cultured fishes. Therefore, screening these candidate genes for polymorphisms reflecting local adaptation or artificial selection is of particular interest for population genetics and aquaculture.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genomic sequences and genetic differentiation at associated tandem repeat markers in growth hormone, somatolactin and insulin-like growth factor-1 genes of the sea bass,Dicentrarchus labrax

Quéré

Guinand

Kuhl

et al. 2010

Aquat. Living Resour.

View full text Add to dashboard Cite

-The completion of genomic sequences of physiologically important genes frequently reveals non-coding genetic elements such as tandem repeats (micro-and minisatellites) that are often more polymorphic than nearby coding sequences. We obtained the complete genomic sequences of three hormone genes in sea bass Dicentrarchus labrax: growth hormone (dlGH), somatolactin (dlSL) and insulin-like growth factor-1 (dlIGF-1), including 5 -and 3 -untranslated regions. Mini-and microsatellites were discovered in both flanking and intron regions. Some were partially conserved across Perciformes. To assess the usefulness and relevance of these gene-associated markers for understanding population structure, an investigation was made on genetic diversity and differentiation at four of them in (i) five wild populations from the North Sea, the Bay of Biscay and the Western Mediterranean, and (ii) two samples of hatchery-bred individuals from a freshwater-acclimation experiment. Gene and allelic diversities were lower in cultured individuals than in wild ones. Significant genetic differentiation was demonstrated between Bay of Biscay + North Sea and Mediterranean populations (F st > 0.06, p < 0.001), primarily due to dlGH-associated markers. Significant genetic differentiation was also detected among the Atlantic and North Sea samples, but restricted to the locus associated with dlSL. Significant genetic differentiation was also found among experimental individuals before and after a salinity challenge (F st ≈ 0.05, p < 0.001), but was due to dlSL and dlIGF-1 loci. Gene-associated markers proved to be more efficient than formerly used anonymous microsatellite markers in providing a clear picture of genetic differentiation.Key words: Growth hormone / Somatolactin / Insulin-like growth factor-1 / Non-coding regions / European sea bass / Microsatellites / Minisatellites Résumé -Connaître les séquences génomiques de gènes d'importance physiologique permet de déceler des élé-ments génétiques non-codants tels que des répétitions en tandem (micro -et minisatellites), souvent porteurs de davantage de polymorphismes que les séquences codantes voisines. Des séquences génomiques complètes ainsi que celles de leurs régions flanquantes en 5 et 3 sont établies pour trois gènes codants pour des hormones chez le bar, Dicentrarchus labrax : l'hormone de croissance (dlGH), la somatolactine (dlSL) et le facteur de croissance apparenté à l'insuline (dlIGF-1). Ainsi, un mini-et des microsatellites partiellement conservés chez les Perciformes ont été trouvés. Pour évaluer la pertinence de ces marqueurs pour des études de structure génétique des populations, leur diversité et leur différenciation génétiques sont examinées chez (i) cinq populations sauvages de la mer du Nord, du golfe de Gascogne et de la Méditerranée occidentale, ainsi que dans ( Resour. 23, 285-296 (2010) pour le locus associé à dlSL. Enfin, pour les marqueurs associés à dlSL et dlIGF-1, une différenciation génétique significative est mise en évidence entre individus expérim...

show abstract

Section: Genetic Profiles Of Populationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genomic sequences and genetic differentiation at associated tandem repeat markers in growth hormone, somatolactin and insulin-like growth factor-1 genes of the sea bass,Dicentrarchus labrax

Quéré

Guinand

Kuhl

et al. 2010

Aquat. Living Resour.

View full text Add to dashboard Cite

show abstract

“…Gen-gen penyandi tumbuh cepat merupakan kandidat utama marka DNA untuk analisis pautan dengan Quantitatif Traits Loci (QTL) disebabkan signifikansi pengaruh biologisnya terhadap sifat-sifat bernilai ekonomis. Pemetaan QTL dengan menggunakan mikrosatelit untuk sifat pertumbuhan pada beberapa hewan vertebrata telah dilaporkan sejumlah peneliti seperti Maskur et al (2007), Geldermann et al (2006), Zhang et al (2009), dan Chaoui et al (2012.…”

Section: (Ahmad Muzaki)unclassified

PERTUMBUHAN DAN SINTASAN LARVA DAN BENIH IKAN KERAPU SUNU Plectropomus leopardus TURUNAN KETIGA (F-3) DARI INDUK HASIL SELEKSI

Muzaki‬

Sembiring²,

Wardana³

et al. 2017

Jurnal Riset Akuakultur

View full text Add to dashboard Cite

Pertumbuhan dan sintasan larva dan benih kerapu sunu turunan ketiga (F-3) dari induk turunan kedua (F-2) hasil seleksi telah diamati di Balai Penelitian dan Pengembangan Budidaya Laut, Gondol. Induk F-2 diseleksi menggunakan marka indikator tumbuh cepat PL-03 (alel tunggal berukuran 370 bp). Penelitian ini bertujuan untuk mengevaluasi keragaan pertumbuhan dan sintasan larva dan benih kerapu sunu F-3 serta pewarisan sifat tumbuh cepat dari induk kerapu sunu (F-2) kepada benih kerapu sunu F-3. Pembenihan menggunakan induk kerapu sunu F-2 dengan sifat tumbuh cepat (membawa marka PL-03) dan tanpa sifat tumbuh cepat. Telur dan larva yang dihasilkan dipelihara sebagai turunan F-3 hingga berukuran benih dan dibesarkan selama 10 bulan pemeliharaan. Hasil pengamatan sintasan larva kerapu sunu F-3 dari induk membawa marka PL-03 adalah sebesar 10,6% ± 9,1%; sedangkan larva F-3 dari induk yang tidak membawa sifat tumbuh cepat hanya sebesar 2,7% ± 1,7%. Benih F-3 dari induk membawa marka PL-03 dapat dipanen saat mencapai ukuran 2,5-3,0 cm pada umur 38 ± 2 hari, sedangkan benih F-3 dari induk tanpa marka PL-03 pada umur 40 ± 1 hari. Selanjutnya benih kerapu sunu F-3 dari induk yang mempunyai marka PL-03 yang dipelihara selama 10 bulan memiliki laju pertumbuhan harian lebih baik (3,11%) dibandingkan benih kerapu sunu F-3 tanpa marka PL-03 (3,01%). Sintasan benih kerapu sunu F-3 dengan marka PL-03 tidak berbeda nyata dengan yang tidak mempunyai marka PL-03. Persentase benih F-3 turunan dari induk F-2 dengan sifat tumbuh cepat yang membawa marka PL-03 sebesar 45%.The growth and survival rate of the third generation (F-3) of coral trout grouper larvae and fry from the genetically selected broodstock were observed at the Institute for Mariculture Research and Development, Gondol. The F-2 broodstock were selected using the fast-growing marker indicator, PL-03 (single allele of 370 bp in size). The aim of this study was to determine the growth performance and survival rate of larvae and fry of F-3 coral trout groupers, as well as inheritance of fast growth trait from the F-2 broodstock to the F-3. The eggs and larvae of F-3 produced from F-2 broodstock with fast growth trait (having PL-03 marker) and without fast growth trait were reared to reach fry stage for 10 months. The results showed that the survival rate of the F-3 larvae from broodstock with PL-03 marker was 10.6% ± 9.1%, while F-3 larvae from the broodstock without fast growth trait only reached 2.7% ± 1.7%. Fry (F-3) with PL-03 marker reached the optimum harvest size of 2.5-3.0 cm at 38 ± 2 days old, while the F-3 fry of broodstock without PL-03 marker took relatively longer to reach that size (40 ± 1 days age). The F-3 from the broodstock with PL-03 marker reared for 10 months had better growth rate (3.11%) compared to F-3 fry without PL-03 marker (3.01%). The survival rate of F-3 fry with PL-03 marker was not significantly different to F-3 fry without PL-03 marker. The percentage of F-3 fry from the broodstock of F-2 with fast growth trait was 45%.

show abstract

“…Assim, o gene produtor deste hormônio se tornou um importante alvo para pesquisas no setor de melhoramento animal, inclusive na aquicultura, setor no qual um dos principais objetivos é maior ganho de peso em menor tempo (HILSDORF; ORFÃO, 2011;MARTIN-SMITH et al, 2004 O gene GH de teleósteos é regulado por interações sinergéticas de um número de fatores regulatórios (Pit-1, CRE, TRE, GRE e ERE) com o promotor (MORIYAMA et al, 2006;ZHANG et al, 2009). Pit-1 (pituitary-specific transcription factor) é um fator de transcrição específico da glândula hipófise.…”

Section: Identificação Dos Snpsunclassified

Avaliação de polimorfismos no gene do Hormônio de Crescimento (GH1) de duas variedades de <i>Oreochromis niloticus</i> e sua associação com caracteristicas de desempenho.

Jaser¹

View full text Add to dashboard Cite

Dedico este trabalho aos meus pais Anis Karim Khalil Jaser e Eucileide Cândida de Morais Jaser e a meu irmão Eyas Anis Karim Jaser, aqueles a quem devo todas as conquistas alcançadas. AGRADECIMENTOSA Deus por me conceder capacidade para alcançar meus objetivos.Aos meus pais pela criação, carinho, apoio e por terem possibilitado meus estudos.Ao meu orientador Prof. Dr. Alexandre Wagner Silva Hilsdorf por ter me integrado a seu grupo de pesquisa e pelos projetos a mim confiados. À Dra. Léia Cecília de Lima Fávaro, com quem tive o privilégio de trabalhar, por toda a atenção e paciência e por ter me ensinado muitas das técnicas empregadas no desenvolvimento deste trabalho. A todo o corpo docente do curso de Ciências Biológicas da UMC, por todos os conhecimentos compartilhados e apoio durante o curso de graduação.A todos os amigos que estiveram ao meu lado dando apoio e carinho.Aos peixes estudados. Muito Obrigada!!! "Quando o homem explorar intensamente o pequeno átomo e o imenso espaço e disser que domina o mundo, quando conquistar as mais complexas tecnologias e disser que sabe tudo, então ele terá tempo para se voltar para dentro de si mesmo." "Descobrirá que se tornou um gigante na ciência, mas que é um frágil menino que não sabe navegar nas águas da emoção e que desconhece os segredos que tecem a colcha de retalhos da sua inteligência."Augusto Cury de associação e seis novos blocos foram identificados (K a P). Considerando todas as pesagens, os blocos "B", "P", "K", "L" e "M" foram associados aos melhores pesos da população de associação e, os SNPs GHP6, GHP7, GHP8, GHP9 e GHP10 demonstraram associação significativa (P < 0,05) como o crescimento, ressaltando que os genótipos de maior valor aditivo para peso foram observados na variedade Chitralada. Portanto, foi possível estimar um conjunto de genótipos com maior efeito aditivo sobre o crescimento da população estuada, o qual poderia ser utilizado em futuros programas de melhoramento genético de tilápia assistidos por marcadores moleculares.Palavras-chave: Tilápia, Hormônio de Crescimento, SNP, Aquicultura. Chitralada (n=100) were used in grow-out testing in cages. The two targeted regions were amplified by PCR using primers designed for it, and then sequenced. The sequences were aligned to check the presence of SNPs in the four Nile tilapia strains. The same approach was used to test the SNPs found in the SNP´s prospection phase in the grow-out testing. Allele and genotype frequencies were estimated for each SNP and genotype. Genotype blocks (set of SNPs genotypes) frequencies were also estimated. Association between SNPs and growth rate were statistically estimated by univariate linear mixed model taking into account fixed and random effects. A total of 10 SNPs were found; nine located in the proximal promoter region and one located in the 5" UTR region, which formed 10 genotype blocks (A to J). Five of these genotype blocks (F to J) were not found in the grow-out individuals. However, six new genotype blocks (K to P) were identified. In all four weighing rec...

show abstract

Nibea coibor growth hormone gene: Its phylogenetic significance, microsatellite variation and expression analysis

Cited by 18 publications

References 43 publications

Genomic sequences and genetic differentiation at associated tandem repeat markers in growth hormone, somatolactin and insulin-like growth factor-1 genes of the sea bass,Dicentrarchus labrax

Genomic sequences and genetic differentiation at associated tandem repeat markers in growth hormone, somatolactin and insulin-like growth factor-1 genes of the sea bass,Dicentrarchus labrax

PERTUMBUHAN DAN SINTASAN LARVA DAN BENIH IKAN KERAPU SUNU Plectropomus leopardus TURUNAN KETIGA (F-3) DARI INDUK HASIL SELEKSI

Avaliação de polimorfismos no gene do Hormônio de Crescimento (GH1) de duas variedades de <i>Oreochromis niloticus</i> e sua associação com caracteristicas de desempenho.

Contact Info

Product

Resources

About