NGS-identified miRNAs in Canine Mammary Gland Tumors Show Unexpected Expression Alterations in qPCR Analysis

Chen, Hui-Wen; Lai, Yu‐Chang; Rahman, Md. Mahfuzur; Husna, Al Asmaul; Hasan, Md Nazmul; Hatai, Hitoshi; Miyoshi, Noriaki; Yamato, Osamu; Miura, Naoki

doi:10.21873/invivo.12873

Cited by 3 publications

(3 citation statements)

References 24 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiR-1, expressed with both upregulation and downregulation in NDP ( 25–28 ) and neoplasia ( 29–36 ), expression in NP ( 37–39 ), and downregulation in IDP ( 40 ), targets both MET in hepatocellular carcinoma ( 33 ) and EDFR in mammary carcinoma ( 35 , 41 ), and was shown to inhibit cell proliferation in a MET dependent manner ( 33 ). MiR-20a, reported to be expressed in NP ( 37 , 42 ), upregulated in various neoplasms ( 7 , 41 , 43 , 44 ), and downregulated in both NDP ( 45 ) and IDP ( 46 ), targets TGF-β affecting epithelial to mesenchymal transition and fibrosis in myxomatous mitral valve disease ( 45 ).…”

Section: Resultsmentioning

confidence: 99%

“…The majority of studies used only a single method of evaluation including reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ( 8 , 10–13 , 16 , 17 , 19 , 27 , 31 , 33 , 36 , 37 , 40–43 , 47 , 49 , 50 , 52 , 54 , 55 , 59 , 60 , 62 , 68–121 , 25 –), microarray ( 44 , 53 , 122–125 ), or next generation sequencing (NGS) ( 32 , 38 , 126–131 ), Some studies evaluated the miRNA through two methods including NGS with validation via RT-qPCR ( 29 , 30 , 35 , 39 , 40 , 45 , 46 , 51 , 56 , 61 , 63 , 65 , 67 , 132–144 ),microarray with validation via RT-qPCR ( 5 , 9 , 14 , 18 , 28 , 57 , 58 , 64 , 66 , 145–149 ), one study utilized NGS with validation using digital drop PCR (ddPCR) ( 7 ), one study utilized nanostring with qRT-PCR ( 20 ), and two studies utilized in situ hybridization (ISH) for validation of their findings ( 39 , 109 ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A review on microRNA detection and expression studies in dogs

Varvil,

dos Santos

2023

Front. Vet. Sci.

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small non-coding RNAs that function by post-transcriptional regulation of gene expression. Their stability and abundance in tissue and body fluids makes them promising potential tools for both the diagnosis and prognosis of diseases and attractive therapeutic targets in humans and dogs. Studies of miRNA expression in normal and disease processes in dogs are scarce compared to studies published on miRNA expression in human disease. In this literature review, we identified 461 peer-reviewed papers from database searches using the terms “canine,” “dog,” “miRNA,” and “microRNA”; we screened 244 for inclusion criteria and then included a total of 148 original research peer-reviewed publications relating to specific miRNA expression in canine samples. We found an overlap of miRNA expression changes between the four groups evaluated (normal processes, non-infectious and non-inflammatory conditions, infectious and/or inflammatory conditions, and neoplasia) in 39 miRNAs, 83 miRNAs in three of the four groups, 110 miRNAs in two of the three groups, where 158 miRNAs have only been reported in one of the groups. Additionally, the mechanism of action of these overlapping miRNAs varies depending on the disease process, elucidating a need for characterization of the mechanism of action of each miRNA in each disease process being evaluated. Herein we also draw attention to the lack of standardization of miRNA evaluation, consistency within a single evaluation method, and the need for standardized methods for a direct comparison.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A review on microRNA detection and expression studies in dogs

Varvil,

dos Santos

2023

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…Although most of the studies conducted in CMTs are based on the latter classification, in 2019, a new histopathological classification for CMTs was published by the Davis-Thompson Foundation [47]. Since then, some studies have used this novel classification to categorize the neoplastic lesions in canine mammary glands [173][174][175][176][177][178][179]. This new classification classifies epithelial neoplastic lesions as either benign or malignant, which are further subdivided into simple, non-simple and ductal-associated tumors.…”

Section: Histological and Molecular Classification Of Breast/mammary ...mentioning

confidence: 99%

Mammary Glands of Women, Female Dogs and Female Rats: Similarities and Differences to Be Considered in Breast Cancer Research

Ferreira

Gama

Seixas

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

Breast cancer is one of the most common and well-known types of cancer among women worldwide and is the most frequent neoplasm in intact female dogs. Female dogs are considered attractive models or studying spontaneous breast cancer, whereas female rats are currently the most widely used animal models for breast cancer research in the laboratory context. Both female dogs and female rats have contributed to the advancement of scientific knowledge in this field, and, in a “One Health” approach, they have allowed broad understanding of specific biopathological pathways, influence of environmental factors and screening/discovery of candidate therapies. This review aims to clearly showcase the similarities and differences among woman, female dog and female rat concerning to anatomical, physiological and histological features of the mammary gland and breast/mammary cancer epidemiology, in order to better portray breast tumorigenesis, and to ensure appropriate conclusions and extrapolation of results among species. We also discuss the major aspects that stand out in these species. The mammary glands of female dogs and women share structural similarities, especially with respect to the lactiferous ducts and lymphatic drainage. In contrast, female rats have only one lactiferous duct per nipple. A comprehensive comparison between humans and dogs is given a special focus, as these species share several aspects in terms of breast/mammary cancer epidemiology, such as age of onset, hormonal etiology, risk factors, and the clinical course of the disease. Holistically, it is clear that each species has advantages and limitations that researchers must consider during the development of experimental designs and data analysis.

show abstract