NGF-induced neurite outgrowth in PC12 cells is independent of calcium entry through L-type calcium channels

Abstract

Neuronal growth factor (NGF) induces neurodifferentiation of PC12 cells into cholinergic neurons-like cells. It was shown that intracellular Ca 2+ ions participate in regulation of the differentiation of PC12 cells. We tested whether L-type calcium channels contribute to Ca 2+ entry which supports neurite outgrowth accompanying NGF-activated differentiation process. Development of morphological changes did correlate with increase of functional expression of L-type calcium channels. However, inhibition of L-type calcium channels by 1 μM of isradipine did not affect significantly an NGF-activated neurite outgrowth.

CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation

“…Ca 2+ , a critical second messenger, is involved in many intracellular molecular signaling cascades [47, 48]. Previous studies have shown that Ca 2+ plays important roles in regulating neurite extension triggered by extracellular factors, such an chemicals, neurotrophin or physical stimulation [5, 49, 50]. In the developing central nervous system (CNS), Ca 2+ regulates neurite extension and retraction and axon guidance [51–53].…”

Reduction of Cav1.3 channels in dorsal hippocampus impairs the development of dentate gyrus newborn neurons and hippocampal-dependent memory tasks

“…LTCCs have been implicated in the neurite outgrowth of immature neuronal cell lines and cultured hippocampal or cortical neurons [74–80]. However it is unclear how LTCC isoforms are involved during neuronal development.…”

TGFβ1 downregulates neurite outgrowth, expression of Ca2+ transporters, and mitochondrial dynamics of in vitro cerebellar granule cells

“…The length of neurites was measured as described previously [18]. Briefly, culture dishes were photographed and pictures were analyzed using a tool 'ruler' in Adobe Photoshop CS2 software.…”

“…Resting membrane potentials and single depolarization-evoked action potentials were measured in the whole-cell configuration of a patch clamp using the HEKA EPC10 amplifier (HEKA Electronics, Lambrecht, Germany) as described previously [18]. Resting membrane potentials and single depolarization-evoked action potentials were measured in the whole-cell configuration of a patch clamp using the HEKA EPC10 amplifier (HEKA Electronics, Lambrecht, Germany) as described previously [18].…”