NFATc1 with AP-3 Site Binding Specificity Mediates Gene Expression of Prostate-specific-membrane-antigen

Lee, Sang Jin; Lee, Kang Ryul; Xiao-sheng, Yang; Jung, Chang-Yeol; Gardner, Thomas A.; Kim, Hong Sup; Jeng, Meei-Huey; Kao, Chinghai

doi:10.1016/s0022-2836(03)00640-5

Cited by 32 publications

(23 citation statements)

References 29 publications

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“…It lacks typical TATA or CAAT boxes but possesses purine-rich sequences, which is in agreement with human and mouse promoters [22]. AiECSOD has an AP-3 binding site which is recognized by the NFAT (nuclear factors in activated T cells) family in immune cells that express cytokine [38]. AiECSOD also has binding sites for Oct-1 and Oct-2.1 which are ubiquitously expressed POU domain transcription factors and are essential for post-translational modification [39,40].…”

Section: Speciessupporting

confidence: 55%

Cloning, characterization, and expression analysis of extracellular copper/zinc superoxide dismutase gene from bay scallop Argopecten irradians

Bao

et al. 2009

Fish & Shellfish Immunology

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Section: Speciessupporting

confidence: 55%

Cloning, characterization, and expression analysis of extracellular copper/zinc superoxide dismutase gene from bay scallop Argopecten irradians

Bao

et al. 2009

Fish & Shellfish Immunology

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“…The consensus binding sequence of AP3 is similar to the binding sites of NFkB and NFATc1 (McCaffrey et al 1992, Lee et al 2003. Therefore, the possibility arises that the AP3 binding activity found in the rat luteal cells could be due to NFkB or NFATc1.…”

Section: Characterization Of the Luteal Ap3 Binding Proteinmentioning

confidence: 99%

Identification of regulatory elements in the Cyp19 proximal promoter in rat luteal cells

Stocco¹,

Kwintkiewicz²,

Zhang³

2007

Journal of Molecular Endocrinology

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The cytochrome P450 aromatase (Cyp19) gene encodes an enzyme of crucial importance in the synthesis of estradiol. Estradiol is luteotropic in the rat. In this species, luteal Cyp19 expression increases progressively during pregnancy and falls before parturition. The mechanisms that control these changes are unknown. Using gel shift assays, we sought to identify the promoter regions that control Cyp19 expression in the rat corpus luteum (CL). The Cyp19 promoter contains a cAMP response element-like sequence (CLS), two nuclear receptor elements half sites (NREs), a GATA binding site, a Yin Yang-1 (YY1) response element, and an activation protein 3 (AP3) binding site. Nuclear extracts were obtained from CL of rats on days 4, 15, and 23 of pregnancy and from the ovaries of immature rats treated with vehicle or a hormone that induces Cyp19 expression in the follicles. CLS was active in immature ovaries but inactive in the CL of pregnant rats, whereas binding to NREs and GATA was observed in both tissues. YY1 was inactive in all samples tested. In the CL, AP3 binding was higher on day 15 of pregnancy when compared with day 4 and day 23 but it was absent in ovaries of immature rats, whereas luteinization increased AP3 binding activity. Mutation of the AP3 site blunted the stimulation of Cyp19 promoter activity in granulosa cells. Our results indicate that CLS is active only in follicles; whereas in the CL, binding to the GATA, NRE, and AP3 sites associates with changes in Cyp19 expression, suggesting that they control Cyp19 promoter activity in luteal cells.

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“…A 10 nmol/L final concentration of R1881 was added. Nuclear extracts were collected as described previously (21). Nuclear extracts (100 g) were preincubated with 4 g of AR-PG21 antibodies (Upstate Biotechnology) for 3 hours at 4°C, followed by adding protein A/G agarose (Santa Cruz Biotechnology).…”

Section: Introductionmentioning

confidence: 99%

HOXB13 Induces Growth Suppression of Prostate Cancer Cells as a Repressor of Hormone-Activated Androgen Receptor Signaling

Jung¹,

Kim²,

Zhang³

et al. 2004

Cancer Research

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128

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NFATc1 with AP-3 Site Binding Specificity Mediates Gene Expression of Prostate-specific-membrane-antigen

Cited by 32 publications

References 29 publications

Cloning, characterization, and expression analysis of extracellular copper/zinc superoxide dismutase gene from bay scallop Argopecten irradians

Cloning, characterization, and expression analysis of extracellular copper/zinc superoxide dismutase gene from bay scallop Argopecten irradians

Identification of regulatory elements in the Cyp19 proximal promoter in rat luteal cells

HOXB13 Induces Growth Suppression of Prostate Cancer Cells as a Repressor of Hormone-Activated Androgen Receptor Signaling

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