NF-κB inhibition attenuates LPS-induced TLR4 activation in monocyte cells

Wan, Jian; Yi, Shuhua; Fan, Yibo; Fan, Conghui; Chen, Song; Sun, Jie; Zhu, Lili; Qin, Long; Yu, Mengjin; Zhou, Lin

doi:10.3892/mmr.2016.5825

Cited by 67 publications

(39 citation statements)

References 16 publications

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“…LPS presentation on macrophages may mediate the activation of the NF-κB pathway ( 21 , 22 , 37 ), leading to the generation of pro-inflammatory cytokines, including IL-6 and TNF-α. We determined the phosphorylation status of NF-κB with and without BDB treatment by western blot analysis.…”

Section: Resultsmentioning

confidence: 99%

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Anti-Inflammatory Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde, a Component ofPolysiphonia morrowii,In VivoandIn Vitro

et al. 2017

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3-Bromo-4,5-dihydroxybenzaldehyde (BDB) is a natural bromophenol compound that is most commonly isolated from red algae. The present study was designed to investigate the anti-inflammatory properties of BDB on atopic dermatitis (AD) in mice induced by 2,4-dinitrochlorobenzene (DNCB) and on lipopolysaccharide (LPS)-stimulated murine macrophages. BDB treatment (100 mg/kg) resulted in suppression of the development of AD symptoms compared with the control treatment (induction-only), as demonstrated by reduced immunoglobulin E levels in serum, smaller lymph nodes with reduced thickness and length, a decrease in ear edema, and reduced levels of inflammatory cell infiltration in the ears. In RAW 264.7 murine macrophages, BDB (12.5, 25, 50, and 100 μM) suppressed the production of interleukin-6, a proinflammatory cytokine, in a dose-dependent manner. BDB also had an inhibitory effect on the phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and signal transducer and activator of transcription 1 (STAT1; Tyr 701), two major signaling molecules involved in cellular inflammation. Taken together, the results show that BDB treatment alleviates inflammatory responses in an atopic dermatitis mouse model and RAW 264.7 macrophages. These results suggest that BDB may be a useful therapeutic strategy for treating conditions involving allergic inflammation such as atopic dermatitis.

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Section: Resultsmentioning

confidence: 99%

“…In a previous study, we reported that N-tosyl-L-phenylalanine chloromethyl ketone, a specific inhibitor of NF-κB signaling, affects IL-6 production ( 23 ). NF-κB inhibition attenuates TLR4 expression in LPS-treated THP1 monocytes ( 22 ). Choi et al .…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde, a Component ofPolysiphonia morrowii,In VivoandIn Vitro

et al. 2017

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“…LPS-induced aggrecan and collagen II downregulation was inhibited by carthamin yellow through the suppression of the MAPK pathway activation (37). LPS stimulates NF-κB binding in the TLR4 gene promoter, whereas TLR4 expression is blocked by NF-κB inhibitors (31,38). Furthermore, LPS was reported to induce inflammation in acute kidney injury (AKI) by activating the TLR4/NF-κB signaling pathway (39).…”

Section: Overexpression Of Mir-140-5p Inhibits Lipopolysaccharideindumentioning

confidence: 99%

Overexpression of miR-140-5p inhibits lipopolysaccharide-induced human intervertebral disc inflammation and degeneration by downregulating toll-like receptor 4

et al. 2018

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Toll-like receptor 4 (TLR4) families are receptors for ligands that initiate extracellular or intracellular signaling, such as lipopolysaccharides (LPS). It has been reported that TLR4 activation resulted in the upregulation of a coordinated set of proinflammatory mediators and inhibition of matrix expression in the intervertebral disc (IVD). miR-140-5p (miR-140) is reported to participate in cellular anti-inflammatory processes and target TLR4. In the present study, we investigated the relationship between TLR4 and miR-140 in IVD degeneration. The expression of TLR4, interleukin (IL)-6, IL-I, L-1β and tumor necrosis factor (TNF)-α was higher, in high-grade IVD degeneration tissues than in low-grade tissues. In contrast, the expression of miR-140, aggrecan and collagen type II was lower in high-grade IVD degeneration tissues than in low-grade IVD degeneration tissues. LPS stimulation resulted in significant increases in TLR4 expression and decreases in miR-140 expression in nucleus pulposus (NP) cells and TLR4 was identified as a target of miR-140 by dual-luciferase reporter assay. The overexpression of miR-140 inhibited the upregulation of the expression of TLR4, TNF-α, IL-1β and IL-6 inflammation cytokines, and the activation of NF-κB and reversed the downregulation of the expression of aggrecan and collagen type II induced by LPS stimulation. In conclusion, the present study may lead to a greater understanding of IVD degeneration and provide new insights into the treatment of this disease.

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“…139 Toll-Like Receptors (TLRs) are strong activators of the NF-κB intracellular pathway. 140 Activation of TLRs on the cells of the periodontium leads to the overstated release of proinflammatory mediators, which may cause host tissue destruction. 141 TLR4 expression is blocked by the NF-κB inhibitor.…”

Section: Nf-κb Signalingmentioning

confidence: 99%

“…141 TLR4 expression is blocked by the NF-κB inhibitor. 140 Neutralization of the NF-κB pathway might provide a useful therapeutic strategy in periodontitis. 142 PDT reduces inflammatory markers NF-kB and inflammatory cytokines.…”

Section: Nf-κb Signalingmentioning

confidence: 99%

The Photomodulation Activity of Metformin Against Oral Microbiome

2019

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Periodontitis is one of the most common inflammatory diseases of the periodontium, which results in the inflammatory destruction of supporting structures around teeth and is closely associated with the development of systemic disease. Due to a wide variety of antibiotic resistance periodontopathic bacteria, photodynamic therapy (PDT) is a non-invasive adjunctive therapeutic modality that is capable of destroying the whole range of microbes. Metformin (Metf) is an antidiabetic drug, and recent studies suggest that cancer patients who receive Metf and are exposed to radiotherapy and chemotherapy show better outcomes. Our surveys in this review introduce Metf as a potent stimulus in increasing the efficacy of PDT in the induction of destruction in microbial cells.

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NF-κB inhibition attenuates LPS-induced TLR4 activation in monocyte cells

Cited by 67 publications

References 16 publications

Anti-Inflammatory Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde, a Component ofPolysiphonia morrowii,In VivoandIn Vitro

Anti-Inflammatory Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde, a Component ofPolysiphonia morrowii,In VivoandIn Vitro

Overexpression of miR-140-5p inhibits lipopolysaccharide-induced human intervertebral disc inflammation and degeneration by downregulating toll-like receptor 4

The Photomodulation Activity of Metformin Against Oral Microbiome

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