Next Generation Sequencing Provides Rapid Access to the Genome of Puccinia striiformis f. sp. tritici, the Causal Agent of Wheat Stripe Rust

Abstract

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Advancing Knowledge on Biology of Rust Fungi Through Genomics

“…tritici (isolate PST-130) was obtained by Illumina-only sequencing (Cantu et al, 2011). A partial assembly of 65 Mb was gathered with a greater sequencing depth compared with the other rust genomes, but at a lower resolution (see number and size of contigs/scaffolds in Table 6.1).…”

Plant exomics: Concepts, applications and methodologies in crop improvement

“…To access the genomic sequences Illumina sequencing was used for highly virulent PST race 130. 89 A plant hostpathogen interaction has also been analyzed using SuperSAGE. SuperSAGE has been applied to rice leaves infected with M. grisea and the analysis of gene expression profiles using entire genomes of both host and the pathogen.…”

“…It is clearly seen that NGS technology has proved to be very efficient and quicker in evaluating the genome sequences for its applications in plant pathology and plant-microbe interaction studies. 89 In order to develop strategies for crop improvement we need to have a deep insight of molecular mechanisms controlling plant immunity. There are basically 2 immune responses in plants governed by PAMP-triggered immunity (PTI).…”

Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence

“…in Australian isolates). A recent publication reported 25 polymorphic genomederived SSRs in Australian Pst isolates (Bailey et al, 2013), and these markers were developed using genomic sequence of North American race PST130 (genome size about 64.8 Mb) published by Cantu et al (2011) and supplemented with sequence data from collaborators at the Australian National University. A total of 1889 SSR loci were identified in these datasets.…”