Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence

Zhan, Gangming; Wang, Fuping; Luo, Huaiyong; Jiang, Shuchang; Zheng, Wenming; Huang, Lili; Kang, Zhensheng

doi:10.1631/jzus.b1400364

Cited by 17 publications

(6 citation statements)

References 29 publications

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“…Ninety six pairs of Pst SSR primers [32][33][34][35][36][37][38] (Table S2) were screened for useful markers. Forward primers were fluorescently 5′-labeled with either FAM, HEX, TAM or ROX.…”

Section: Ssr Primers Pcr Amplification Electrophoresis and Data Anamentioning

confidence: 99%

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of Puccinia striiformis f. sp. tritici

Tian¹,

Zhan²,

Lu³

et al. 2017

Front. Agr. Sci. Eng.

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Wheat stripe rust caused by Puccinia striiformis f. sp. tritici is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of P. striiformis f. sp. tritici was selfed on Berberis shensiana to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing Yr genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with Yr5, Yr10, Yr15, Yr24 and Yr26 and virulent on lines with Yr17, Yr25 and YrA, indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with Yr1,

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Section: Ssr Primers Pcr Amplification Electrophoresis and Data Anamentioning

confidence: 99%

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of Puccinia striiformis f. sp. tritici

Tian¹,

Zhan²,

Lu³

et al. 2017

Front. Agr. Sci. Eng.

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“…Mantel test based on genetic distance with 12 SSR marker loci, and virulence distance indicated that the correlation coefficient between the virulence and the SSR marker data was low (R 2 = 0.212 to 0.370), similar to those by Chen (1993), Zhan et al (2015), and Zhan et al (2016). In those studies, virulence and molecular marker data were less significantly correlated or had low correlations.…”

Section: Discussionmentioning

confidence: 82%

Race composition and genetic diversity of a Puccinia striiformis f. sp. tritici population from Yunnan and Guizhou epidemiological regions in China in 2018

et al. 2022

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Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is an extremely destructive wheat disease worldwide. Yunnan and Guizhou provinces are the main overwintering regions for Pst and inoculum sources for the disease in China. Surveillance of race dynamics of the Pst population is essential for managing the disease in local and other wheat-growing regions of China. However, Pst population dynamics in this region is not monitored yearly. In this study, a Pst population of 113 isolates from Yunnan and Guizhou in 2018 were phenotyped on the two wheat differential sets and analyzed by 12 simple sequence repeat (SSR) markers. As a result, 25 races were identified from the Pst population on Chinese differentials and predominant races was CYR32 with a frequency of 17.70%. None of the isolates was virulent to Yr5 and Yr15. Only a few isolates showed virulence to YrTr1 and differentials Zhong 4. The Kosman index ( K ) of the Pst population was 0.292 on the Chinese differentials, and 0.274 on single Yr gene lines and additional differentials, respectively. In total, 64 MLGs were detected among 113 isolates and the expected heterozygosity ( He ) was 0.424. A close genetic relationship was detected between Lufeng (Yunnan) / Nayong (Guizhou), Fuyuan (Yunnan) / Bijie (Guizhou), and Shizong (Yunnan) / Xingyi (Guizhou). This study provided useful information on population structure and virulence to Yr genes in the Yunnan and Guizhou epidemiological regions, and will be used to guide the control of wheat stripe rust and targeted wheat breeding in this region. Supplementary Information The online version contains supplementary material available at 10.1007/s42161-022-01273-1.

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“…DNA samples were diluted to 50 ng/μL for SSR amplification. Twelve pairs of published SSR primers ( 57 – 60 ) were used to genotype every isolate (see Table S1). The materials and specific experimental procedures described by Wang et al ( 51 ) were followed.…”

Section: Methodsmentioning

confidence: 99%

Genetic Relationships of Puccinia striiformis f. sp. tritici in Southwestern and Northwestern China

et al. 2022

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Understanding the epidemiology and population genetics of plant pathogens is crucial to formulate efficient predictions of disease outbreaks and achieve sustainable integrated disease management, especially for pathogens with migratory capability. Here, this study covers the genetic homogeneity and heterogeneity of different geographical Pst populations on broad to fine spatial scales from the key epidemic regions of the two agroecosystems in China, where wheat stripe rust occurs annually.

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Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence

Cited by 17 publications

References 29 publications

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of Puccinia striiformis f. sp. tritici

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of Puccinia striiformis f. sp. tritici

Race composition and genetic diversity of a Puccinia striiformis f. sp. tritici population from Yunnan and Guizhou epidemiological regions in China in 2018

Genetic Relationships of Puccinia striiformis f. sp. tritici in Southwestern and Northwestern China

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