Next-Generation Sequencing Methods

Rajesh, T.; Jaya, M.

doi:10.1016/b978-0-444-63667-6.00007-9

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…The major advances are such as high speed, cell-free library construction, ability to run thousands to millions of sequencing reactions in parallel, direct detection without the need for electrophoresis, and sequencing in real time. These technologies are becoming a potential tool for gene expression analysis, especially for those species having reference genome sequences already available time (Rajesh and Jaya 2017).…”

Section: Next-generation Sequencingmentioning

confidence: 99%

“…Whereas each microbead is subjected to successive cycles of ligation, signature identification, and cleavage, the use of the flow cell ensures that all the microbeads remain in a closely packed monolayer. Fluorescent signals from the array of microbeads are acquired onto a CCD camera leading to a digital representation of each microbead, and image-processing software then tracks the positions of fluorescent signals from individual microbeads in the flow cell (Brenner et al 2000;Rajesh and Jaya 2017).…”

Section: Massively Parallel Signature Sequencing (Mpss)mentioning

confidence: 99%

“…Massively parallel signature sequencing is a high-throughput sequencing technology, based in the combination of in-vitro cloning of template DNA onto microbeads by making a complex mixture of template oligonucleotide tag conjugates, followed by cycles of ligation-based DNA sequencing (Rajesh and Jaya 2017).…”

Section: Massively Parallel Signature Sequencing (Mpss)mentioning

confidence: 99%

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Review of Molecular Techniques for the Identification of Bacterial Communities in Biological Effluent Treatment Facilities at Pulp and Paper Mills

et al. 2017

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One of the processes most used in biotechnology today for handling industrial liquid wastes is biological wastewater treatment. The efficiency and quality of its operation depends on the composition and activity of the microbial community that is present. The application of traditional and molecular techniques has provided a glimpse into the "black box" and has given information to improve the wastewater treatment process. However, bleach pulp and paper mill effluents require a better understanding of the active bacterial population. For the study of these microorganisms, molecular techniques have been used for more than 15 years. However, there has been a lack of knowledge of the physiological requirements and relations with the environment, which seems to be very difficult to obtain involving profile on the diversity. Nowadays, highthroughput sequencing technology is a promising method that makes it possible to identify the entire profile of microbial communities. In combination with fingerprint methods, this approach allows the identification and analysis of the whole biodiversity of microbial communities. In this review, several identification techniques will be discussed.

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Section: Next-generation Sequencingmentioning

confidence: 99%

Section: Massively Parallel Signature Sequencing (Mpss)mentioning

confidence: 99%

Section: Massively Parallel Signature Sequencing (Mpss)mentioning

confidence: 99%

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Review of Molecular Techniques for the Identification of Bacterial Communities in Biological Effluent Treatment Facilities at Pulp and Paper Mills

et al. 2017

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“…Isolation and fragmentation of DNA, library preparation, massive parallel sequencing, bioinformatics analysis, and variant/mutation annotation and interpretation are the major steps involved in DNA based NGS ( Qin, 2019 ). Massively parallel signature sequencing, pyrosequencing, polony sequencing, and sequencing by oligonucleotide ligation detection (SOLID) are some commonly available advanced sequencing methods in HTS ( Rajesh and Jaya, 2017 ). RNA-Sequencing (RNA-Seq) offers advanced coverage and greater resolution of the dynamic nature of the transcriptome.…”

Section: Molecular Tools For Detection Of Fungimentioning

confidence: 99%

Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review

Hariharan¹,

Prasannath²

2021

Front. Cell. Infect. Microbiol.

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Phytopathogenic fungal species can cause enormous losses in quantity and quality of crop yields and this is a major economic issue in the global agricultural sector. Precise and rapid detection and identification of plant infecting fungi are essential to facilitate effective management of disease. DNA-based methods have become popular methods for accurate plant disease diagnostics. Recent developments in standard and variant polymerase chain reaction (PCR) assays including nested, multiplex, quantitative, bio and magnetic-capture hybridization PCR techniques, post and isothermal amplification methods, DNA and RNA based probe development, and next-generation sequencing provide novel tools in molecular diagnostics in fungal detection and differentiation fields. These molecular based detection techniques are effective in detecting symptomatic and asymptomatic diseases of both culturable and unculturable fungal pathogens in sole and co-infections. Even though the molecular diagnostic approaches have expanded substantially in the recent past, there is a long way to go in the development and application of molecular diagnostics in plant diseases. Molecular techniques used in plant disease diagnostics need to be more reliable, faster, and easier than conventional methods. Now the challenges are with scientists to develop practical techniques to be used for molecular diagnostics of plant diseases. Recent advancement in the improvement and application of molecular methods for diagnosing the widespread and emerging plant pathogenic fungi are discussed in this review.

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