Next Generation Sequence Analysis

Sub-Lethal Effects of Pesticides on the DNA of Soil Organisms as Early Ecotoxicological Biomarkers

“…Among the available NGS technologies, Illumina is nowadays mostly applied because of throughput, data quality, and costs per sequenced nucleotide. The use of NGS in recent years has increased in many scientific fields (e.g., Clinical Research, Food Science, Agricultural Science, Toxicology) as it became cheaper and therefore accessible to most labs all over the world (Beedanagari and John, 2014;Rockmann et al, 2019;Wiedmann and Carroll, 2019). The technology provides a thorough depiction of soil microbiology, paving the way for the discovery of previously unexplored compositions and diversities of both culturable and unculturable soil microorganisms (Simon and Daniel, 2011;Thompson et al, 2017).…”