Newly Fabricated Magnetic Lanthanide Oxides Core–Shell Nanoparticles in Phosphoproteomics

Abstract

Metal oxides show high selectivity and sensitivity toward mass spectrometry based enrichment strategies. Phosphopeptides/phosphoproteins enrichment from biological samples is cumbersome because of their low abundance. Phosphopeptides are of interest in enzymes and phosphorylation pathways which lead to the clinical links of a disease. Magnetic core-shell lanthanide oxide nanoparticles (Fe3O4@SiO2-La2O3 and Fe3O4@SiO2-Sm2O3) are fabricated, characterized by SEM, FTIR, and EDX and employed in the enrichment of phosphopeptides. The nanoparticles enrich phosphopeptides from casein variants, nonfat milk, egg yolk, human serum and HeLa cell extract. The materials and enrichment protocols are designed in a way that there are almost no nonspecific bindings. The selectivity is achieved up to 1:8500 using β-casein/BSA mixture and sensitivity down to 1 atto-mole. Batch-to-batch reproducibility is high with the reuse of core-shell nanoparticles up to four cycles. The enrichment followed by MALDI-MS analyses is carried out for the identification of phosphopeptides from serum digest and HeLa cell extract. Characteristic phosphopeptides of phosphoproteins are identified from human serum after the enrichment, which have the diagnostic potential toward prostate cancer. Thus, the lanthanide based magnetic core-shell materials offer a highly selective and sensitive workflow in phosphoproteomics.

Enrichment of HDL proteome and phospholipidome from human serum via IMAC/MOAC affinity

“…Cellulose–SiO 2 –IMAC–Sm 3+ (Najam‐ul‐Haq, Jabeen, Shafiq, Sajid, & Saba, ), SiO 2 –La 2 O 3 composite (Jabeen et al, ) and Fe 3 O 4 @Sm 2 O 3 core–shell nanocomposites (Jabeen et al, ) were synthesized using the reported protocols.…”

Recent Advances in the Synthesis, Surface Modifications and Applications of Core‐Shell Magnetic Mesoporous Silica Nanospheres

“…are often used to enrich phosphopeptides. [167] For example, Zhang's group [168] synthesized adenosineÀ Ti 4 + functionalized mesoporous graphene oxide nanospheres (MG@mSiO2À ATPÀ Ti 4 + ) to selectively extract phosphorylated peptides from complex biological samples. As a result, MG@mSiO2À ATPÀ Ti 4 + has excellent surface properties, good hydrophilicity, size exclusion effect, excellent sensitivity and selectivity to phosphopeptides.…”

Nanomaterials in Proteomics

“…Aside from the common metal oxides, lanthanide oxides are also explored to capture phosphoproteins/phosphopeptides. Jabeen et al prepared two magnetic core–shell lanthanide oxides of Fe 3 O 4 @SiO 2 –La 2 O 3 and Fe 3 O 4 @SiO 2 –Sm 2 O 3 recently, the successive hydroxyl groups on which can form bidentate ligand coordination with phosphopeptides . Both of the magnetic lanthanide oxides exhibited high selectivity when using β‐casein/bovine serum albumin (BSA) peptide mixture at ratio of 1:8500 as test sample, as well as high sensitivity of 0.001 fmol, owing to the affinity ability of electrostatic, covalent, and bidentate coordination provided by Sm/La (III).…”