New Variants of Porcine Epidemic Diarrhea Virus, China, 2011

Li, Wentao; Li, Heng; Liu, Yunbo; Pan, Ye; Deng, Feng; Song, Yanhua; Tang, Xibiao; He, Qigai

doi:10.3201/eid1803.120002

Cited by 348 publications

(235 citation statements)

References 13 publications

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“…Since then, it has been reported in many European and Asian countries. In the past ten years, new outbreaks of PED in Thailand [3], China [4,5,6], South Korea [7,8], USA [9], and Japan [10] have caused serious economic losses to their swine industry. For example, the PED outbreak in USA in 2013 led to an estimated death of 7 million piglets [11].…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteome Analysis of Porcine Jejunum Tissues in Response to a Virulent Strain of Porcine Epidemic Diarrhea Virus and Its Attenuated Strain

Chen

et al. 2016

Viruses

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Porcine epidemic diarrhea virus (PEDV), a predominant cause of acute enteric infection, leads to severe dehydrating diarrhea and mortality in piglets all over the world. A virulent PEDV YN13 strain, isolated in our laboratory, was attenuated to yield an attenuated PEDV strain YN144. To better understand the pathogenesis mechanism and the virus-host interaction during infection with both PEDV YN13 and YN144 strains, a comparative proteomic analysis was carried out to investigate the proteomic changes produced in the primary target organ, using isobaric tags for relative and absolute quantitation (iTRAQ) labeling, followed by liquid chromatography tandem-mass spectrometry (LC-MS/MS). A total of 269 and 301 differently expressed proteins (DEPs) were identified in the jejunum tissues of the piglets inoculated with YN13 and YN144, respectively. Bioinformatics analysis revealed that these proteins were involved in stress responses, signal transduction, and the immune system. All of these involved interferon-stimulated genes (ISGs) which were up-regulated in jejunums by both of the PEDV-infected groups. Based on the comparative analysis, we proposed that different changes induced by YN13 and YN144 in heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1), eukaryotic initiation factor 4G1 (eIF4G1), and some members in the heat shock protein (HSP) family, may be responsible for differences in their pathogenicity.

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Section: Introductionmentioning

confidence: 99%

Comparative Proteome Analysis of Porcine Jejunum Tissues in Response to a Virulent Strain of Porcine Epidemic Diarrhea Virus and Its Attenuated Strain

Chen

et al. 2016

Viruses

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“…Therefore, the diversity of PEDV strains may be studied based on the S gene. However, most studies have only assessed the partial or full S genes, with the full S protein characterization being conducted rarely Li et al, 2012;Gao et al, 2013). In this study, the complete nucleotide and deduced peptide sequences of S protein genes of the four Gansu PEDV field strains were compared to those of published PEDV reference strains, and the S protein characteristics were predicted.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the S gene of PEDV is considered to be the most useful in revealing the genetic diversity of PEDV (Chang et al, 2002;Bosch et al, 2003;Nam and Lee, 2010;Park et al, 2011a;Shirato et al, 2011;Li et al, 2012). Therefore, the diversity of PEDV strains may be studied based on the S gene.…”

Section: Discussionmentioning

confidence: 99%

“…Since the 2010s, there have been continuous outbreaks of this disease in swine farms in nearly all provinces in China (Chen et al, 2010;Sun et al, 2012;Wang et al, 2013;Zhang et al, 2013;Zhao et al, 2013;Jung and Saif, 2015;Song et al, 2015a). Specifically, during the initial prevalence of the disease in farrowing barns, the mortality of newborn piglets has been found to be approximately 100%, which is responsible for considerable financial losses faced by the swine industry (Shibata et al, 2000;Li et al, 2012;Jung and Saif, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Although most large-scale pig farms were vaccinated according to the proper immune program, immunized swine herds continue to be infected by PEDV (Li et al, 2012;Zhao et al, 2013;Jung and Saif, 2015;Song et al, 2015b) (Li et al, 2012;Zhao et al, 2013;Jung and Saif, 2015;Song et al, 2015b). Therefore, the genetic relationships between different strains must be elucidated to effectively control and prevent PEDV infection.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus samples obtained from farms in Gansu, China

Wang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Porcine epidemic diarrhea poses significant sanitation problems in the porcine industry, and has negatively affected the economy in recent years. In this study, 48 fecal specimens were collected from piglets from four intensive swine farms located in the Gansu Province of China. The molecular diversity and phylogenetic relationships between porcine epidemic diarrhea viruses (PEDV) prevalent in Gansu were probed, and the resultant proteins were characterized. Sequence analysis of the spike protein (S) genes showed that each specimen had unique characteristics, and that the PEDV1/S/4 strain could be differentiated from the others via a unique mutation of the S gene. The phylogeny of S glycoprotein showed that all strains were clustered into two major groups. The four Gansu PEDV field strains were characterized into different groups; this finding was consistent with the results of the protein characterization prediction. This analysis additionally revealed the unique characteristics of each specimen. The results of this study could be used to elucidate the prevalence of PEDV and contribute to the prevention of PEDV in Gansu.

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