New trends for automation in immunoassays

Truchaud, A.; Capolaghi, B.; Yvert, J. P.; Gourmelin, Y.; Glikmanas, G; Bogard, M.

doi:10.1351/pac199163081123

Cited by 8 publications

(3 citation statements)

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“…The synthesis of ultrasmall magnetic nanoparticles with uniform size distribution is very important because of their wide applications in biology and medicine, [1][2][3][4][5][6][7][8][9] for example, magnetic resonance imaging (MRI) contrast agent, 5,6,9 magnetic separation of oligonucleotides, 7 cells, and other biocomponents, 7 magnetically guided site specific drug delivery systems, 3,4 and so forth. The industrial application of magnetic materials covers a broad range.…”

Section: Introductionmentioning

confidence: 99%

“…The synthesis of ultrasmall magnetic nanoparticles with uniform size distribution is very important because of their wide applications in biology and medicine, − for example, magnetic resonance imaging (MRI) contrast agent, ,, magnetic separation of oligonucleotides, cells, and other biocomponents, magnetically guided site specific drug delivery systems, , and so forth. The industrial application of magnetic materials covers a broad range. − The strongly magnetizable fluids find numerous applications, ,− for example, magnetic seals in motors and devices such as gas lasers, blowers, and so forth, lubricants and bearings in magnetic disk drive spindles, optical memory devices, magnetic inks for bank checks, magnetic refrigeration units, and an efficient material for magnetic recording media.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and Characterization of Silica-Coated Iron Oxide Nanoparticles in Microemulsion: The Effect of Nonionic Surfactants

et al. 2001

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A water-in-oil microemulsion method has been applied for the preparation of silica-coated iron oxide nanoparticles. Three different nonionic surfactants (Triton X-100, Igepal CO-520, and Brij-97) have been used for the preparation of microemulsions, and their effects on the particle size, crystallinity, and the magnetic properties have been studied. The iron oxide nanoparticles are formed by the coprecipitation reaction of ferrous and ferric salts with inorganic bases. A strong base, NaOH, and a comparatively mild base, NH4OH, have been used in each surfactant to observe whether the basicity has some influence on the crystallization process during particle formation. Transmission electron microscopy, X-ray electron diffraction, and superconducting quantum interference device magnetometry have been employed to study both uncoated and silica-coated iron oxide nanoparticles. All these particles show magnetic behavior close to that of superparamagnetic materials. By use of this method, magnetic nanoparticles as small as 1-2 nm and of very uniform size (percentage standard deviation is less than 10%) have been synthesized. A uniform silica coating as thin as 1 nm encapsulating the bare nanoparticles is formed by the base-catalyzed hydrolysis and the polymerization reaction of tetraethyl orthosilicate in microemulsion. All experimental results are also compared with those for particles synthesized in pure water.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Silica-Coated Iron Oxide Nanoparticles in Microemulsion: The Effect of Nonionic Surfactants

et al. 2001

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“…3 In contrast, homogeneous immunoassays generally contain two analytical steps (i.e. reagent addition and measurement) similar to chemisical assays, 4 which can be adapted in conventional chemistry analyzers. Meanwhile, the homogeneous assay mode can be amendable to automation, reduce the risk of contamination and eliminate time-consuming washing steps or sample handling.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalized dendritic polyaniline nanofibers for sensitive electrochemical immunoassay of biomarkers

Cui

Tang

Liu

et al. 2012

Analyst

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Multi-armed dendritic polyaniline nanofibers (MPANFs) were first synthesized and functionalized with horseradish peroxidase (HRP) and carcinoembryonic antibody (anti-CEA) for highly efficient electrochemical immunoassay of carcinoembryonic antigen (CEA, as a model analyte here) in this work. Transmission electron microscope (TEM) and scanning electron microscope (SEM) techniques were employed to characterize the synthesized MPANFs. By using anti-CEA-conjugated core-shell gold-Fe(3)O(4) nanocomposites (GoldMag) as immunosensing probes and biofunctionalized MPANFs as molecular tags, a new sandwich-type homogeneous immunoassay strategy was developed for the determination of CEA by coupling with a home-made flow-through magneto-controlled microfluidic device. Under optimal conditions, the electrochemical immunoassay exhibited a wide dynamic range of four orders of magnitude from 1.0 pg mL(-1) to 50 ng mL(-1) with a low detection limit of 0.1 pg mL(-1) CEA at 3σ. Intra- and inter-assay coefficients of variation were below 10%. The assayed results for clinical serum specimens with the electrochemical immunoassay were received in good accordance with the results obtained from the referenced enzyme-linked immunosorbent assay (ELISA) method.

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Homogeneous Two-Site Immunometric Assay Kinetics as a Theoretical Tool for Data Analysis

Zuber

Mathis²,

Flandrois

1997

Analytical Biochemistry

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New trends for automation in immunoassays

Abstract: Abstract

Cited by 8 publications

References 0 publications

Synthesis and Characterization of Silica-Coated Iron Oxide Nanoparticles in Microemulsion: The Effect of Nonionic Surfactants

Synthesis and Characterization of Silica-Coated Iron Oxide Nanoparticles in Microemulsion: The Effect of Nonionic Surfactants

Biofunctionalized dendritic polyaniline nanofibers for sensitive electrochemical immunoassay of biomarkers

Homogeneous Two-Site Immunometric Assay Kinetics as a Theoretical Tool for Data Analysis

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