New Transport Medium for Shipment of Clinical Specimens I

Cary, Sylvia G.; Blair, Eugene B.

doi:10.1128/jb.88.1.96-98.1964

Cited by 124 publications

(38 citation statements)

References 4 publications

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“…Cary-Blair medium, unlike EX, does not contain antibiotics, but was also shown to have a very good sensitivity, enabling recovery of the organism from 90% and 93% of the boot swab samples, before and after enrichment, respectively. The low oxidation reduction potential of this medium ensures a high pH is maintained that may promote the repair of injured cells that are sensitive to low pH (Cary and Blair, 1964). Cary-Blair medium has been previously shown to improve the transport conditions and subsequent survival of C. jejuni (Luechtefeld et al, 1981) and presents the advantage of having a longer shelf-life and lower cost compared with EX.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Vidal

Rodgers

Arnold

et al. 2012

Zoonoses and Public Health

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The objective of the study was to evaluate the performance of different combinations of sample type, transport medium and culture methods for the recovery of Campylobacter jejuni and C. coli from broiler flocks at primary production. Boot swabs moistened with one of four different transport media [maximum recovery diluent (n=120), Exeter broth (EX) (n=120), buffered peptone water (n=120) and modified semi-solid Cary-Blair (n=120)], caecal samples (n=40) and faecal samples (n=120) from 40 broiler flocks were compared and sensitivity estimates obtained using a Bayesian model. Samples were cultured onto mCCDA before and after enrichment in EX and incubated microaerobically at 41.5°C. Campylobacter suspect colonies were identified to the species level by multiplex PCR. Results from the Bayesian model indicated that boot swabs after enrichment had higher sensitivity (90-94%) than caecal contents before or after enrichment (84% and 89%, respectively) and faecal samples after enrichment (82%) for the detection of Campylobacter spp., although these differences were not statistically significant. Enrichment significantly increased the sensitivity of boot swab and caecal samples for detection of Campylobacter spp. and C. jejuni, respectively. However, the enrichment of caecal samples resulted in a significant decrease in the sensitivity of these samples for detection of C. coli. There was much greater variation in the sensitivity estimates of the methods for detecting C. coli than for C. jejuni, and the ranking of methods was different between the two species. Boot swabs gave the best sensitivity values for detection of C. jejuni, and enrichment culture of faecal samples was the most sensitive method for detection of C. coli.

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Section: Discussionmentioning

confidence: 99%

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Vidal

Rodgers

Arnold

et al. 2012

Zoonoses and Public Health

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“…For the sock samples, tubular retention bandage (Danafast) was used. The bandage was moistened with 20 ml Cary-Blair transport medium (Cary and Blair 1964;Luechtefeld et al 1981), before sampling and pulled over ordinary shoes. The socks were used for sampling of the ground or floor outside and inside the broiler houses.…”

Section: Sampling At Farm Levelmentioning

confidence: 99%

Correlations between Campylobacter spp. prevalence in the environment and broiler flocks

et al. 2007

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Aims: To investigate (i) possible correlations between the presence of Campylobacter spp. in the surroundings of broiler farms and their incidence in flocks, and (ii) possible associations between weather conditions and the occurrence of Campylobacter spp. Methods and Results: Farms were selected according to previous results from the Swedish Campylobacter programme. Samples were collected in and around broiler houses during the rearing period from 131 flocks on 31 farms, including sock samples from the ground outside, from the floor in the broiler houses and anterooms, and samples from insects, water, feed and ventilation shafts. Conclusions: As expected, there was a difference in Campylobacter isolation rates for different categories of farms regarding samples taken in the houses. However, there were no differences regarding the presence of Campylobacter spp. in the environment between producers that often deliver Campylobacter‐positive slaughter batches and those that rarely deliver positive batches. Campylobacter spp. were more frequently found in the surroundings on rainy days when compared with sunny days. Significance and Impact of the Study: Physical barriers between outside and inside the houses appeared to be important for preventing Campylobacter spp. in the environment to be transferred into the broiler houses.

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“…The last two types are equivalent to the routine samples taken in the monitoring programme for Campylobacter in broilers in Sweden. The samples were placed in capped plastic tubes containing 10 ml of Cary-Blair transport medium (Cary and Blair 1964) with 0AE16% agar (Luechtefeld et al 1981). All samples collected on Monday to Thursday were sent by post without refrigeration, together with an identification report and arrived the following day at the laboratory.…”

Section: Collection Of Samplesmentioning

confidence: 99%

Transmission of Campylobacter spp. to chickens during transport to slaughter

Hansson

Ederoth

Andersson³

et al. 2005

J Appl Microbiol

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Aims: To determine the prevalence of Campylobacter-contaminated transport crates and to determine whether contaminated crates represent a risk for contamination of chickens during transport to slaughter. Methods and Results: Samples were collected from cleaned transport crates before they were dispatched to the farms. Chicken groups were sampled within 24 h before transport to slaughter and at the slaughterhouse. Campylobacter spp. were isolated from 69 of 122 (57%) sampled batches of transport crates. Twenty-six slaughter groups, negative at farm level, were transported in batches of crates from which Campylobacter spp. had been isolated. In 11 (42%) of these 26 slaughter groups, Campylobacter spp. were found in samples taken at slaughter. The corresponding figure for at-farm-negative slaughter groups transported in negative crates was four (15%) testing positive at slaughterhouse of 27 slaughter groups [relative risk (RR) ¼ 2AE9, 95% CI 1AE1-7AE3]. In four of 11 slaughter groups, genetic subtyping by pulsed-field gel electrophoresis was able to support the hypothesis of contamination from crates to chickens during transport to slaughter. Conclusions: Despite washing and disinfection, crates were frequently contaminated with Campylobacter and it could have contaminated chickens during transport to slaughter. Significance and Impact of the Study: Campylobacter-positive crates are a risk factor for chickens testing campylobacter-positive at slaughter.

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New Transport Medium for Shipment of Clinical Specimens I

Cited by 124 publications

References 4 publications

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Comparison of Different Sampling Strategies and Laboratory Methods for the Detection of C. jejuni and C. coli from Broiler Flocks at Primary Production

Correlations between Campylobacter spp. prevalence in the environment and broiler flocks

Transmission of Campylobacter spp. to chickens during transport to slaughter

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