New Therapeutic Approaches for the Treatment of Brucella Infections: Gentamicin Entrapment into Drug Delivery Systems

Gamazo, Carlos; Blanco-Prieto, María José; Lecaroz, Maria Concepcion; Vitas, Ana Isabel; Gander, Bruno; Irache, Juan M.; Prior, Sandra

doi:10.2174/1568012043354125

Cited by 8 publications

(3 citation statements)

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“…In addition to this evaluation of data, one had to take into account that nanoparticles are known to stimulate the macrophages [30,31,32]. As an increase in unspecific fluorescence was seen, an inhibition of Toxoplasma growth by unloaded nanoparticles was not surprising.…”

Section: Tabmentioning

confidence: 99%

Effects of nanoparticles in cells infected by Toxoplasma gondii

et al. 2012

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Core-shell model drug carriers on 2 nanoscale size levels have been applied in cell culture studies, focused on Toxoplasmosis therapy. In synthesis, a seed of Rhodamin B-labelled polystyrene latex particles was coated by polybutyl cyanoacrylate under physical inclusion of 2 different, new drugs against Toxoplasmosis. Drug-loaded as well as drug-free core-shell model drug carriers were added to a cell culture of human macrophages, infected by Toxoplasma gondii, following an infection plan. Drug release from the carriers had been studied before by enzymatic degradation by means of pork liver esterase. Particle size decrease by degradation was investigated in a UV/VIS spectrometer via transmission measurements. Drug release profiles were obtained by HPLC studies. The dynamics in the population of infected human macrophages, Toxoplasma gondii as well as model drug carrier numbers were registered by a FACS (fluorescence-activated cell sorter). As main result, the drug-free references in the 2 series of core-shell model drug carriers achieved ca.85% of the observed maximum in Toxoplasmosis therapy efficiency. These data were correlated with an immune stimulant effect on the side of the human macrophages, caused by the cell uptake of colloidal substrate, foreign to the body.

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Section: Tabmentioning

confidence: 99%

Effects of nanoparticles in cells infected by Toxoplasma gondii

et al. 2012

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“…By measuring 10,000 cells, FACS offered the opportunity to statistically treat these data in different ways, resulting in a more complex interpretation. In addition to this evaluation of data, one had to take into account that nanoparticles are known to stimulate the macrophages [30][31][32]. As an increase in unspecific fluorescence was seen, an inhibition of Toxoplasma growth by unloaded nanoparticles was not surprising.…”

Section: Resultsmentioning

confidence: 99%

Effects of nanoparticles in cells infected by Toxoplasma gondii

Leyke¹,

Köhler-Sokolowska²,

Paulke³

et al. 2016

Nano Online

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Core-shell model drug carriers on 2 nanoscale size levels have been applied in cell culture studies, focused on Toxoplasmosis therapy. In synthesis, a seed of Rhodamin B-labelled polystyrene latex particles was coated by polybutyl cyanoacrylate under physical inclusion of 2 different, new drugs against Toxoplasmosis. Drug-loaded as well as drug-free core-shell model drug carriers were added to a cell culture of human macrophages, infected by Toxoplasma gondii, following an infection plan. Drug release from the carriers had been studied before by enzymatic degradation by means of pork liver esterase. Particle size decrease by degradation was investigated in a UV/VIS spectrometer via transmission measurements. Drug release profiles were obtained by HPLC studies.The dynamics in the population of infected human macrophages, Toxoplasma gondii as well as model drug carrier numbers were registered by a FACS (fluorescenceactivated cell sorter).As main result, the drug-free references in the 2 series of core-shell model drug carriers achieved ca.85% of the observed maximum in Toxoplasmosis therapy efficiency.These data were correlated with an immune stimulant effect on the side of the human macrophages, caused by the cell uptake of colloidal substrate, foreign to the body.Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.

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“…In addition to this evaluation of data, one had to take into account that nanoparticles are known to stimulate the macrophages [30][31][32]. As an increase in unspecific fluorescence was seen, an inhibition of Toxoplasma growth by unloaded nanoparticles was not surprising.…”

Section: Effects Of Nanoparticles Inmentioning

confidence: 99%

Effects of Nanoparticles in Cells Infected by Toxoplasma gondii

Silja¹,

Paulke²,

Presber³

2017

Toxoplasmosis

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Core-shell model drug carriers on two nanoscale size levels have been applied in cell culture studies and focused on Toxoplasmosis therapy. In synthesis, a seed of rhodamin B-labelled polystyrene latex particles was coated by polybutyl cyanoacrylate under physical inclusion of two different new drugs against Toxoplasmosis. Drug-loaded and drug-free core-shell model drug carriers were added to a cell culture of human macrophages, infected by Toxoplasma gondii, following an infection plan. Drug release from the carriers had been studied before by enzymatic degradation by means of pork liver esterase. Particle size decrease by degradation was investigated in a UV/VIS spectrometer via transmission measurements. Drug release profiles were obtained by HPLC studies.The dynamics in the population of infected human macrophages, T. gondii as well as model drug carrier numbers were registered by an FACS (fluorescence-activated cell sorter). As main result, the drug-free references in the two series of core-shell model drug carriers achieved ca.85% of the observed maximum in Toxoplasmosis therapy efficiency. These data were correlated with an immune stimulant effect on the side of the human macrophages, caused by the cell uptake of colloidal substrate, foreign to the body.

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New Therapeutic Approaches for the Treatment of Brucella Infections: Gentamicin Entrapment into Drug Delivery Systems

Cited by 8 publications

References 0 publications

Effects of nanoparticles in cells infected by Toxoplasma gondii

Effects of nanoparticles in cells infected by Toxoplasma gondii

Effects of nanoparticles in cells infected by Toxoplasma gondii

Effects of Nanoparticles in Cells Infected by Toxoplasma gondii

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