Effects of Nanoparticles in Cells Infected by Toxoplasma gondii

Abstract

Core-shell model drug carriers on two nanoscale size levels have been applied in cell culture studies and focused on Toxoplasmosis therapy. In synthesis, a seed of rhodamin B-labelled polystyrene latex particles was coated by polybutyl cyanoacrylate under physical inclusion of two different new drugs against Toxoplasmosis. Drug-loaded and drug-free core-shell model drug carriers were added to a cell culture of human macrophages, infected by Toxoplasma gondii, following an infection plan. Drug release from the carriers had been studied before by enzymatic degradation by means of pork liver esterase. Particle size decrease by degradation was investigated in a UV/VIS spectrometer via transmission measurements. Drug release profiles were obtained by HPLC studies.The dynamics in the population of infected human macrophages, T. gondii as well as model drug carrier numbers were registered by an FACS (fluorescence-activated cell sorter). As main result, the drug-free references in the two series of core-shell model drug carriers achieved ca.85% of the observed maximum in Toxoplasmosis therapy efficiency. These data were correlated with an immune stimulant effect on the side of the human macrophages, caused by the cell uptake of colloidal substrate, foreign to the body.

Isolation of Human Small Extracellular Vesicles and Tracking of Their Uptake by Retinal Pigment Epithelial Cells In Vitro

“…In another study, two different drugs against ocular toxoplasmosis were successfully loaded into polystyrene latex particles during polymerization, generating core-shell nanoparticles [27]. However, the drug-loaded particles were no more effective than the unloaded particles.…”

Effects of nanoparticles in cells infected by Toxoplasma gondii

“…Polystyrene cores were prepared by emulsion polymerisation of styrene in a water/ethanol mixture (16:1; v/v) in the presence of rhodamine B and purified by dialysis against deionised water. One gram of these core particles in water with a pH of 2 was used as seed for the polymerisation of 2 g butyl cyanoacrylate [38]. In the case of pentamidine loading, 5 mg pentamidine/g polymer was added to the polymerisation medium.…”

“…Nanoparticles (NPs) are a tool for specifically targeting drugs or diagnostics to selected tissues or cell types [1][2][3]. Their use in cancer research and therapy is of special interest [4][5][6][7][8][9] as well as to overcome the blood-brain barrier [10,11]. At least in theory, they are a tool to optimise pharmacological data such as drug release, tissue specificity and even cell specificity [2].…”