New technique for producing hybridoma by using laser radiation

Ohkohchi, Nobuhiro; Itagaki, Hideo; Doi, H.; Taguchi, Yoshio; Satoh, Susumu; Satoh, S.

doi:10.1002/1096-9101(2000)27:3<262::aid-lsm8>3.0.co;2-q

Cited by 12 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, the possibility of leaking small encapsulated molecules during the fusion decreases considerably. Previously, UV laser light was used to fuse individual cells, , and generally, multiple pulses had to be used in conjunction with poly(ethylene glycol), a chemical known to ease and, depending on the concentration, induce membrane fusion. In our experiments, a single UV pulse was sufficient to induce the fusion of liposomes suspended in a buffer solution without the addition of any membrane-destabilizing chemicals.…”

Section: Introductionmentioning

confidence: 99%

Optical Manipulation and Fusion of Liposomes as Microreactors

et al. 2003

View full text Add to dashboard Cite

We present an all-optical method to manipulate and fuse giant liposomes, typically 10 μm in diameter. Optical tweezers are used to trap two individual liposomes, which are then brought into contact. A single pulse of ultraviolet laser light focused on the adjoining membranes induces the fusion between the liposomes. As a consequence, the chemical reagents encapsulated in the two liposomes mix and a chemical reaction takes place.

show abstract

Section: Introductionmentioning

confidence: 99%

Optical Manipulation and Fusion of Liposomes as Microreactors

et al. 2003

View full text Add to dashboard Cite

show abstract

“…(20) It has also been reported that 5% PEG is sufficient to cause cell surface adhesion. (24) In conclusion, we have successfully proven each critical step in B-cell targeting on the basis of immunofluorescence. The application of this technology to in vitro immunization systems should bring about substantive progress for selective yielding of monoclonal antibodies in the short term.…”

Section: Discussionmentioning

confidence: 89%

Antigen-Based Immunofluorescence Analysis of B-Cell Targeting: Advanced Technology for the Generation of Novel Monoclonal Antibodies with High Efficiency and Selectivity

et al. 2006

View full text Add to dashboard Cite

On the basis of immunofluorescence analysis, an entire pathway of B-cell targeting was successfully identified, which can drive selective production of monoclonal antibodies with high efficiency and selectivity. The technique comprises three critical steps, antigen-based preselection of B lymphocytes, formation of antigenselected B lymphocyte and myeloma cell complexes, and selective fusion of B-cell-myeloma cell complexes with electrical pulses. Intriguingly, expression of surface immunoglobulin receptors on B lymphocytes was recognized even after immunization in vitro. The number of the antigen-selected B lymphocytes after in vitro immunization was in fact higher than that obtained after in vivo immunization, suggesting that such shortterm immunization is applicable for B-cell targeting. Immunofluorescence analysis revealed the targeting technique to demonstrate fivefold to tenfold higher efficiency for formation of hybridoma cells than a polyethylene glycol (PEG)-mediated method. This efficiency is in good agreement with production of hybridoma cells secreting desired monoclonal antibodies determined by an enzyme-linked immunosorbent assay (ELISA). The addition of a low concentration of PEG brought about enhanced fusion efficiency and reduced cell damage at even electric intensities as high as 4.0 kV/cm and 5.0 kV/cm. Here we demonstrate that immunofluorescence analysis can successfully clarify an entire pathway of B-cell targeting and provide advantages over the PEG-mediated method. This advanced technology may be applicable for rapid production of monoclonal antibodies based on in vitro immunization.

show abstract

“…The liposomes were brought into close contact using optical tweezers and made to fuse via a single high‐energy ultraviolet laser pulse, resulting in chemical reactions to occur between reactants in the different liposomes. In contrast to other studies which implement ultraviolet laser light to fuse individual cells, [164,165] their study was able to achieve this with a single pulse without the use of any membrane‐destabilizing chemicals. Shiomi et al [166] .…”

Section: Application Of Membrane Fusion Modelsmentioning

confidence: 85%

Membrane Fusion Models for Bioapplications

Mazur

Chandrawati

2021

ChemNanoMat

View full text Add to dashboard Cite

Membrane fusion is a highly evolved and significant physiological process involving the mixing of two initially distinct lipid bilayer membranes. It is concerned with communication between and within cells and plays a key role in various processes such as exocytosis, endocytosis, fertilization, viral infection, and carcinogenesis. Due to its significant role, artificial model systems using lipid membranes have been developed over the last few decades which study the membrane fusion mechanism on a molecular level. More recently, this field of research has shifted its attention towards the use of these developed models for a diverse range of biomolecular and biomedical applications. This review will focus on current applications which stem from these models including drug delivery, sensing, protocell proliferation, and others. Our opinions on the future advancements of this field will also be included.

show abstract

New technique for producing hybridoma by using laser radiation

Abstract: This new cell fusion method is characterized by production of hybridomas of target cells, lower cell toxicity, and a high rate of hybrid production.

Cited by 12 publications

References 11 publications

Optical Manipulation and Fusion of Liposomes as Microreactors

Optical Manipulation and Fusion of Liposomes as Microreactors

Antigen-Based Immunofluorescence Analysis of B-Cell Targeting: Advanced Technology for the Generation of Novel Monoclonal Antibodies with High Efficiency and Selectivity

Membrane Fusion Models for Bioapplications

Contact Info

Product

Resources

About