New Spectrophotometric and Conductometric Methods for Macrolide Antibiotics Determination in Pure and Pharmaceutical Dosage Forms Using Rose Bengal

Abstract

Two Simple, accurate, precise, and rapid spectrophotometric and conductometric methods were developed for the estimation of erythromycin thiocyanate (I), clarithromycin (II), and azithromycin dihydrate (III) in both pure and pharmaceutical dosage forms. e spectrophotometric procedure depends on the reaction of rose bengal and copper with the cited drugs to form stable ternary complexes which are extractable with methylene chloride, and the absorbances were measured at 558, 557, and 560 nm for (I), (II), and (III), respectively. e conductometric method depends on the formation of an ion-pair complex between the studied drug and rose bengal. For the spectrophotometric method, Beer's law was obeyed. e correlation coefficient ( 2 ) for the studied drugs was found to be 0.9999. e molar absorptivity ( ), Sandell's sensitivity, limit of detection (L�D), and limit of quanti�cation (L��) were also calculated. e proposed methods were successfully applied for the determination of certain pharmaceutical dosage forms containing the studied drugs Submit your manuscripts at

Spectrophotometric Determination of Macrolides Using Bromocresol Green in Pharmaceutical Formulations and Urine Samples

“…The detection limits are lower than the reported methods 11,14 . These methods utilized a single step reaction with no extraction process and so simpler compared to reported methods 14,16 .…”

“…It has been observed that, the BCG methods have wider linear ranges as compared to most of the reported methods [11][12][13][14] . These proposed methods are more sensitive compared to some of the established methods as shown by the molar absorptivity 11,14,17 . The detection limits are lower than the reported methods 11,14 .…”

“…These proposed methods are more sensitive compared to some of the established methods as shown by the molar absorptivity 11,14,17 . The detection limits are lower than the reported methods 11,14 . These methods utilized a single step reaction with no extraction process and so simpler compared to reported methods 14,16 .…”

Voltammetric behavior of erythromycin ethylsuccinate at a renewable silver-amalgam film electrode and its determination in urine and in a pharmaceutical preparation

“…Aside from the mentioned highly sophisticated detector, diode-array (DAD) [20,21], chemiluminescence [22], fluorescence [23] and electrochemical [3] detectors have also been used. Additionally, spectrophotometric and conductometric methods for the estimation of erythromycin thiocyanate in both pure and pharmaceutical dosage forms were developed [24].…”

Azithromycin-molecularly imprinted polymer based on PVC membrane for Azithromycin determination in drugs using coated graphite electrode

“…HPLC method has been developed and validated by Trivedi and Patel [22] to measure the amount of Azin in pharmaceutical formulation. Sayed et al [23] developed spectrophotometric and conductivity methods for the estimation of Azin and other antibiotics in both pure and pharmaceutical forms. Sensitive and selective LC-MS methods were developed by Zuak et al [24] and validated for the quantification of Azin in human plasma.…”