New Specific Reagent for Keto-Sugars

Johanson, R.

doi:10.1038/172956a0

Cited by 51 publications

(10 citation statements)

References 2 publications

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“…Carbohydrates were separated by one-dimensional chromatography on silica gel plates employing the solvent system of 1-butanol (9) : acetic acid (6) ; diethyl ether (3); water (1) (Hay, Lewis & Smith, 1963). The spots were located with thymol (Adachi, 1965) and the presence of ketose confirmed with the following reagents: dimedone (Adachi, 1964), anthrone (Johanson, 1953), /f-indolylacetic acid (Heyrovsky, 1956) and phloroglucinol (Menzies & Seakins, 1969). 'Free' and 'bound' carbohydrates were separated by the addition of ethanol to the uterine flushings as described by Mann & Rottenburg (1966) for human semen.…”

Section: Quantitative Analysis Of Uterine Flushingsmentioning

confidence: 99%

Delayed Implantation in Roe Deer (Capreolus Capreolus)

Aitken¹

1974

Reproduction

133

120

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The results presented in this paper indicate that delayed implantation in the roe deer is due to a lack of certain essential factors which are needed to induce and support the process of embryonic growth. These factors are eventually supplied, in the first weeks of January, as a secretion emanating from the endometrial glands. This secretion contains uterine-specific and serum proteins, about twenty free amino acids, protein-bound glucose and galactose and, rather surprisingly, a free ketose which appears to be fructose. Elongation of the roe deer blastocyst is also correlated with a rise in the concentration of plasma oestrogens, an endocrine change that may stimulate the endometrial glands into secretory activity. However, since simultaneous changes were not observed in the ovaries, the elevated oestrogen levels may be a consequence rather than a cause of embryonic growth.

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Section: Quantitative Analysis Of Uterine Flushingsmentioning

confidence: 99%

Delayed Implantation in Roe Deer (Capreolus Capreolus)

Aitken¹

1974

Reproduction

133

120

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“…The polysaccharides gave no coloration with iodine and did not reduce Fehling's solution. No characteristic ltetose coloration was produced on treating with the anthrone reagent of Johanson (11). They all gave a positive carbazole test (2) for uronic acids.…”

Section: (A) Observations On the Chemical Composition Of The Polysaccmentioning

confidence: 99%

The Production of Polysaccharides by Fungi Active in the Decomposition of Wood and Forest Litter

Bernier¹

1958

Can. J. Microbiol.

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Four fungi known to be active in the decomposition of wood and forest litter were grown on a liquid medium containing salts and g l~~c o s e and also on aqueous estracts of a variety of fresh leaves and litter. The polysaccharides produced in the various cult~lre media by the same organism were found, by chromatography of the hydrolyzates, to be of the same composition. In some cases, when the fungi were grown on leaf or litter extracts, polysaccharide synthesis did not take place because of lack of carbohydrate material. In many instances polysaccharide synthesis was inhibited by a factor or factors present even in much diluted extracts.'Manuscript

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“…DNA was determined by a modification of the diphenylamine procedure (Burton, 1956) and by a fluorescent method using ethidium bromide (Prasad er al., 1972). The orcinol procedure (Ogur & Rosen, 1950) was used to measure RNA; carbohydrate was estimated by the anthrone procedure (Johanson, 1953). Protein was determined by the Lowry method.…”

Section: Methodsmentioning

confidence: 99%