New sensitive discovery histoculture model for growth-inhibition studies in prostate cancer and BPH

Olbina, G.; Miljković, D.; Hoffman, Robert M.; Geller, Jack

doi:10.1002/(sici)1097-0045(19981001)37:2<126::aid-pros9>3.0.co;2-e

Cited by 15 publications

(3 citation statements)

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“…More recently, we extended it to the culture of cervicovaginal 23–25 and rectosig-moid tissues 16,26,27 , as these tissues are critical in the transmission of HIV infection and play an important role in HIV pathogenesis. In principle, this technique could also be adapted to the study of other tissues, both normal and cancerous 28 . More systematic studies are required to optimize this technique and to understand its limitations.…”

Section: Introductionmentioning

confidence: 99%

Use of human tissue explants to study human infectious agents

2009

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The study of human cell–cell and cell–pathogen interactions that occur in the context of complex tissue cytoarchitecture is critical for deciphering the mechanisms of many normal and pathogenic processes. This protocol describes methods for culturing and infecting explants of human tissues to study the pathogenesis of human infectious agents and their local interactions. The protocol relies on the use of fresh human tissues dissected into small blocks or biopsies that are cultured at the liquid–air interface on collagen rafts. These tissue blocks retain their cytoarchitecture and support productive infection of various pathogens without exogenous stimulation. Experimental details for setting up cultures of human tonsils, lymph nodes and cervicovaginal and rectosigmoid tissues, including protocols for their infection with HIV-1 and other pathogens, are described here. Using this protocol, culture and infections can be set up in 3–6 h and be maintained for 2–3 weeks, depending on the tissue used.

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Section: Introductionmentioning

confidence: 99%

Use of human tissue explants to study human infectious agents

2009

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“…To our knowledge, anti-growth effects on human BPH tissues by genistein were first reported in 1998 [70], while other investigators further confirmed such genistein-induced growth inhibition on BPH [71]. In 2001, intake of a phytoestrogen-rich diet containing isoflavones was reported to decrease prostate weight in rats [72].…”

Section: Isoflavonesmentioning

confidence: 85%

Pharmacological Effects and Potential Clinical Usefulness of Polyphenols in Benign Prostatic Hyperplasia

et al. 2021

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Benign prostatic hyperplasia (BPH) is arguably the most common benign disease among men. This disease is often associated with lower urinary tract symptoms (LUTS) in men and significantly decreases the quality of life. Polyphenol consumption reportedly plays an important role in the prevention of many diseases, including BPH. In recent years, in addition to disease prevention, many studies have reported the efficacy and safety of polyphenol treatment against various pathological conditions in vivo and in vitro. Furthermore, numerous studies have also revealed the molecular mechanisms of the antioxidant and anti-inflammatory effects of polyphenols. We believe that an improved understanding of the detailed pharmacological roles of polyphenol-induced activities at a molecular level is important for the prevention and treatment of BPH. Polyphenols are composed of many members, and their biological roles differ. In this review, we first provide information regarding the pathological roles of oxidative stress and inflammation in BPH. Next, the antioxidant and anti-inflammatory effects of polyphenols, including those of flavonoids and non-flavonoids, are discussed. Finally, we talk about the results and limitations of previous clinical trials that have used polyphenols in BPH, with particular focus on their molecular mechanisms of action.

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“…We therefore undertook a study of the effects of genistein, a major component of soy, on growth of human-patient benign prostatic hyperplasia (BPH) and prostate cancer tissue in three-dimensional sponge-gel supported histoculture (SSH) (Hoffman, 1998). More recently, we improved the sensitivity of the histoculture method by the use of total immersion in the histoculture medium; we have called this technique total immersion histoculture (TIH) (Olbina et al, 1998).Surgical specimens of human BPH and cancer were histocultured with the SSH and TIH techniques in parallel for 5 days to study the effect of genistein on growth, as measured by inhibition of 3-H-thymidine incorporation per microgram of protein on day 5. …”

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confidence: 99%

Effect of Genistein and Genistein Analogs on Growth of Human Prostate Tissue In Vitro

Geller¹,

Olbina²,

Miljković³

et al. 1999

Journal of Medicinal Food

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that prostate disease is significantly less prevalent in Asia, where the intake of soy products is very high, than in the United States, where intake is low (Gu et al., 1994). We therefore undertook a study of the effects of genistein, a major component of soy, on growth of human-patient benign prostatic hyperplasia (BPH) and prostate cancer tissue in three-dimensional sponge-gel supported histoculture (SSH) (Hoffman, 1998). More recently, we improved the sensitivity of the histoculture method by the use of total immersion in the histoculture medium; we have called this technique total immersion histoculture (TIH) (Olbina et al, 1998).Surgical specimens of human BPH and cancer were histocultured with the SSH and TIH techniques in parallel for 5 days to study the effect of genistein on growth, as measured by inhibition of 3-H-thymidine incorporation per microgram of protein on day 5. RESULTS AND DISCUSSIONWe compared inhibition of growth after treatment of BPH and cancer tissue with genistein in SSH or TIH. Inhibition of growth was expressed as percentage of control growth of the tissue treated with dihydrotestosterone (DHT) alone. Incorporation of 3H-thymidine dramatically increased in control histocultures, by twofold to fivefold, in TIH compared with the same tissue in SSH (Fig. 1).We observed significantly increased growth inhibition of BPH and cancer tissue treated with 18.4 /xmol/L of genistein with TIH, compared with SSH. The average inhibition in TIH was 73% (range 67-79%), compared with a mean decrease of 31% (range 6-68%) in SSH (Fig. 2). This inhibition was statistically significant (P < .05).To examine the sensitivity of TIH, we treated BPH and cancer tissue in TIH with decreasing concentrations of genistein. We observed 40% growth inhibition of tissue treated with 4.6 /¿mol/L and 26% inhibition with 2.3 /¿mol/L, with a mean coefficient of variation (CV) of 13%. Both of these values represent significant differences (P < .05). In contrast, inhibition was not significant with concentrations lower than 9.2 ¿imol/L genistein in SSH (CV range, 8-29%) (Fig. 3).Inhibitory effects on 3H-thymidine incorporation of 20 ¿tmol/L of hydroxyflutamide, a known antiandrogen, were compared using the same tissue in SSH and TIH. In all experiments, the incorporation of 3H-thymidine was three to four times higher for TIH in control histocultures. The inhibition caused by 2 x 10-5 mol/L hydroxyflutamide was 40-60% in TIH. In SSH, inhibition by 2 X 10-5 mol/L hydroxyflutamide was 17-18%, and in some experiments it was not detectable to all.

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New sensitive discovery histoculture model for growth-inhibition studies in prostate cancer and BPH

Cited by 15 publications

References 15 publications

Use of human tissue explants to study human infectious agents

Use of human tissue explants to study human infectious agents

Pharmacological Effects and Potential Clinical Usefulness of Polyphenols in Benign Prostatic Hyperplasia

Effect of Genistein and Genistein Analogs on Growth of Human Prostate Tissue In Vitro

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