New sensitive discovery histoculture model for growth‐inhibition studies in prostate cancer and BPH

Abstract

BACKGROUND.A new, total-immersion three-dimensional histoculture (TIH) method was developed to evaluate growth of tissue containing a mixture of benign prostate hyperplasia (BPH) and prostate cancer in vitro. METHODS. Efficacy of inhibitors, such as genistein, was determined by measuring 3 Hthymidine incorporation per microgram protein. Inhibitory effects obtained in TIH were compared to those in sponge-gel supported histoculture (SSH). RESULTS.3 H-thymidine incorporation was 2-5-fold higher in tissue cultured in TIH than in SSH. The average inhibition by genistein at a concentration of 18 M was 73% in TIH, vs. 31% in SSH. TIH also appeared to be more sensitive, since the lowest concentration of genistein that significantly inhibited growth of BPH mixed with prostate cancer tissue was 2.3 M, while in SSH the lowest concentration was 9.2 M. Although the within-assay coefficient of variation (CV) was similar for both TIH and SSH, the between-assay CV was better in TIH. CONCLUSIONS. These data suggest that TIH can be used as a discovery model for screening and evaluating inhibitors of prostate tissue growth in vitro.

A patient‐derived explant (PDE) model of hormone‐dependent cancer

“…2C). The time-dependent increase in proliferation observed here and in previous organotypic culture models (Nevalainen et al, 1993;Olbina et al, 1998) is likely due to growth-promoting factors in the media or to the release of an inhibitory serum influence that was present in vivo, but absent ex vivo.…”

Selective growth of epithelial basal cells from human prostate in a three‐dimensional organ culture

“…For 3 weeks, at intervals of 7 days, the morphology of the prostatic tissue was evaluated and compared with that of similar samples, cultured after adding DHT to the medium. Our 3D-culture method greatly extends the lifespan of the normal tissue and preserves the gland structure beyond that obtained with most other traditional methods [39][40][41][42][43]. Similar to our method, Parrish et al [44] when culturing precision-cut human prostate slices as an alternative in vitro model of investigating human prostate pathobiology, recently provided another histotypic system, maintaining organ architecture, epithelial and stromal organization and cellular interactions for 5 days.…”