New Records of Microcystins in Some Bulgarian Water Bodies of Health and Conservational Importance

Pavlova, Věra; Stoyneva, Maya P.; Georgieva, Vesela; Donchev, D; Spoof, Lisa; Meriluoto, Jussi; Bratanova, Zlatka; Karadjova, Irina

doi:10.4236/jwarp.2014.65044

Cited by 12 publications

(30 citation statements)

References 19 publications

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“…Likewise, in Lake Kastoria (Greece), M. wesenbergii dominant bloom containing toxin producing genes such as mcyA and mcyB was reported (Gkelis et al, 2014). Pavlova et al (2014; found toxic bloom dominated by M. wesenbergii in Lake Dourankoulak, and highlighted that toxicity may vary between clones of the same strain. Because of these contradictory results, it is necessary to analyse higher number of Microcystis mor- It is known that P. agardhii and P. rubescens have specific ecological niches.…”

Section: Discussionmentioning

confidence: 95%

Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

et al. 2017

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Blooms of cyanobacteria are an increasingly frequent phenomenon in freshwater ecosystems worldwide as a result of eutrophication. Many species can produce hepatotoxins that cause severe health hazards to humans. The aim of this study was to identify the bloom forming cyanobacteria species by molecular methods and to amplify genes responsible for hepatotoxin biosynthesis from the environmental samples and isolated strains of cyanobacteria from Küçükçekmece Lagoon, Sapanca, İznik, Manyas and Taşkısı Lakes. A total of 10 bloom samples and 11 isolated strains were examined and Microcystis spp., Planktothrix spp., Nodularia spumigena, Anabaenopsis elenkinii, Sphaerospermopsis aphanizomenoides, Cylindrospermopsis raciborskii were identified. Hepatotoxin genes were detected in 60% of the bloom samples and 45% of the strains. Two Microcystis strains were obtained from Küçükçekmece Lagoon. While the strain assigned to Microcystis flosaquae was non-toxic, Microcystis aeruginosa strain produced microcystin. According to PCR results, the M. aeruginosa and Planktothrix agardhii bloom samples of Küçükçekmece Lagoon contained the microcystin synthetase gene E (mcyE) indicative of microcystin production, however, no microcystin was detected by HPLC. The mcyE gene was also found in Microcystis wesenbergii isolated from Taşkısı Lake, and in all Planktothrix rubescens bloom samples from Sapanca Lake. To our knowledge, this is the first detailed study for identifiying different toxic cyanobacteria species and their hepatotoxin production from several waterbodies in Turkey using molecular methods.

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Section: Discussionmentioning

confidence: 95%

Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

et al. 2017

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“…2). According to Pavlova et al (2006Pavlova et al ( , 2007Pavlova et al ( , 2013aPavlova et al ( , 2014, Pavlova (2007) and Georgieva et al (2015) (Pavlova, 2007;Pavlova et al, 2007) and LA type was reported once from Borovitsa by Teneva et al (2010b).…”

Section: Environmental Gradients and Cyanoprokaryotes Distributionmentioning

confidence: 97%

“…This study compiles and evaluates the currently available data on phytoplankton and relevant environmental data on Bulgarian WBs (mainly reservoirs and lakes) gathered during the period 2000-2015 (Stoyneva, 2003(Stoyneva, , 2010(Stoyneva, , 2014(Stoyneva, , 2015Beshkova and Botev, 2004;Kalchev et al, 2004;Traykov, 2005;Pavlova et al, 2006Pavlova et al, , 2013aPavlova et al, , 2014Pavlova et al, , 2015Beshkova et al, 2008aBeshkova et al, , 2008bBeshkova et al, , 2012Pavlova, 2007;Tsanev and Belkinova, 2008;Teneva et al, 2009Teneva et al, , 2010aTeneva et al, , 2010bTeneva et al, , 2011Teneva et al, , 2014Cheshmedjiev et al, 2010aCheshmedjiev et al, , 2013Stoyanov et al, 2012Stoyanov et al, , 2013Stoyanov et al, , 2016Stoyanov, 2014Stoyneva et al, 2013Belkinova et al, 2014;Dimitrova et al, 2014aDimitrova et al, , 2014bDochin andStoyneva, 2014, 2015;Dochin, 2015;Georgieva et al, 2015).…”

Section: Data Collection On Planktonic Cyanoprokaryotesmentioning

confidence: 99%

“…The assessment of cyanotoxins registered in the country in relation to their potential producers is based on the works of Pavlova (2007), Pavlova et al (2006Pavlova et al ( , 2007Pavlova et al ( , 2013aPavlova et al ( , 2014Pavlova et al ( , 2015, Teneva et al (2009Teneva et al ( , 2010aTeneva et al ( , 2010bTeneva et al ( , 2011Teneva et al ( , 2014, Stoyanov et al (2012) and Georgieva et al (2015) -Tabs. 2 and 3.…”

Section: Collection and Assessment Of Cyanotoxin Data Algal Blooms Amentioning

confidence: 99%

“…2 and 3. For this study the biomass of the species published earlier by Pavlova et al (2006Pavlova et al ( , 2014 with their cell numbers per liter (or milliliter) was estimated (Tabs. 2 and 3).…”

Section: Collection and Assessment Of Cyanotoxin Data Algal Blooms Amentioning

confidence: 99%

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Assessment of cyanoprokaryote blooms and of cyanotoxins in Bulgaria in a 15-years period (2000-2015)

et al. 2017

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The scientific and public awareness of hazardous photosynthetic prokaryotes (cyanobacteria/cyanoprokaryotes) and especially the contamination of drinking-water reservoirs with cyanotoxins is world-wide increasing. Recently much more attention has been paid to the events and results of mass proliferation of these toxic organisms even in South-East European countries in spite of the fact that, as a rule, they are not controlled by national legislation.The present paper presents a summary of results of such studies carried out in summer-autumn periods of the last 15 years (2000-2015) in Bulgarian water bodies differing by location, morphometry and trophic status, incl. drinking water reservoirs, recreational lakes and sites of nature conservation importance. A multivariate analysis allowed to outline the distribution patterns and environmental drivers of the planktonic cyanoprokaryote assemblages in relation with the available data on the water bodies, highlighting species composition and abundance of the main taxa, including potentially toxic species. Samples analysis by HPLC-DAD and/or LC/MS, ELISA and in vitro cytotoxicity tests allowed detection of microcystins, nodularins and saxitoxins. Toxin concentration ranged between 0.1 and 26.5 µg L-1 in water samples and between 10.9 and 1070 µg g-1 (d.w.) in concentrated (net) samples. Despite the fact that microcystins were not found in all studied water bodies and that the recorded levels were still lower in comparison with some other European countries, the fact that cyanotoxins were detected in 16 water bodies (incl. 3 drinking water reservoirs) could serve as an alert for the need of recognition of cyanotoxins as a new health risk factor in the country. Therefore, permanent monitoring with identification of toxins in water bodies at risk and activities for limitation and control of toxic blooms are urgently needed, in combination with increase of the attention to the effects of cyanotoxins on both human health and health of aquatic ecosystems in Bulgaria.

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Appendix 2: Cyanobacteria Associated With the Production of Cyanotoxins

Bernard

Ballot

Thomazeau

et al. 2016

Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis

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New Records of Microcystins in Some Bulgarian Water Bodies of Health and Conservational Importance

Cited by 12 publications

References 19 publications

Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

Assessment of cyanoprokaryote blooms and of cyanotoxins in Bulgaria in a 15-years period (2000-2015)

Appendix 2: Cyanobacteria Associated With the Production of Cyanotoxins

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