Appendix 2: Cyanobacteria Associated With the Production of Cyanotoxins

Bernard, Cécile; Ballot, Andreas; Thomazeau, Solène; Maloufi, Selma; Furey, Ambrose; Mankiewicz-Boczek, J.; Pawlik‐Skowrońska, Barbara; Capelli, Camilla; Salmaso, Nico

doi:10.1002/9781119068761.app2

Cited by 57 publications

(60 citation statements)

References 225 publications

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“…As recently reviewed by Bernard et al (2017), ten cyanobacterial genera detected in our study (Tabs. 1-4, Supplementary Tab.…”

Section: Discussionsupporting

confidence: 66%

“…Cyanobacteria from Geitlerinema genus have been recently reported to produce MCs (Bernard et al, 2017;Myers et al, 2007;Valdor and Aboal 2007). However, other putative producers of MCs were also present in the Kpong reservoir, most notably P. agardhii, which also penetrated into the final stage of the water treatment on the sampling days when intracellular MCs were detected in the final water (March 10 th , April 8 th ).…”

Section: Discussionmentioning

confidence: 99%

“…However, other putative producers of MCs were also present in the Kpong reservoir, most notably P. agardhii, which also penetrated into the final stage of the water treatment on the sampling days when intracellular MCs were detected in the final water (March 10 th , April 8 th ). The Weija Reservoir had a high diversity of cyanobacteria, including Anabaenopsis tanganyikae (G. S. WEST) WOLOSZYNSKA & V. V. MILLER, A. nygaardii, A. nubilum, M. tenuissima, M. aeruginosa, P. agardhii or P. recta, which are not only species representing the genera potentially producing MCs (Bernard et al, 2017), but they were also detected in the final treated water containing intracellular MCs (February 9 th ).…”

Section: Discussionmentioning

confidence: 99%

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Cyanobacteria and microcystin contamination in untreated and treated drinking water in Ghana

Addico¹,

Hardege²,

Kohoutek³

et al. 2017

Adv Ocean Limnol

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Although cyanobacterial blooms and cyanotoxins represent a worldwide-occurring phenomenon, there are large differences among different countries in cyanotoxin-related human health risk assessment, management practices and policies. While national standards, guideline values and detailed regulatory frameworks for effective management of cyanotoxin risks have been implemented in many industrialized countries, the extent of cyanobacteria occurrence and cyanotoxin contamination in certain geographical regions is underreported and not very well understood. Such regions include major parts of tropical West and Central Africa, a region constisting of more than 25 countries occupying an area of 12 million km 2 , with a total population of 500 milion people. Only few studies focusing on cyanotoxin occurrence in this region have been published so far, and reports dealing specifically with cyanotoxin contamination in drinking water are extremely scarce. In this study, we report seasonal data on cyanobacteria and microcystin (MC) contamination in drinking water reservoirs and adjacent treatment plants located in Ghana, West Africa. During January-June 2005, concentrations of MCs were monitored in four treatment plants supplying drinking water to major metropolitan areas in Ghana: the treatment plants Barekese and Owabi, which serve Kumasi Metropolitan Area, and the plants Kpong and Weija, providing water for Accra-Tema Metropolitan Area. HPLC analyses showed that 65% samples of raw water at the intake of the treatment plants contained intracellular MCs (maximal detected concentration was 8.73 µg L -1 ), whereas dissolved toxins were detected in 33% of the samples. Significant reduction of cyanobacterial cell counts and MC concentrations was achieved during the entire monitoring period by the applied conventional water treatment methods (alum flocculation, sedimentation, rapid sand filtration and chlorination), and MC concentration in the final treated water never exceeded 1 µg L -1 (WHO guideline limit for MC-LR in drinking water). However, cyanobacterial cells (93-3,055 cell mL -1 ) were frequently found in the final treated water and intracellular MCs were detected in 17% of the samples (maximal concentration 0.61 µg L -1 ), while dissolved MCs were present in 14% of the final treated water samples (maximal concentration 0.81 µg L -1 ). It indicates a borderline efficiency of the water treatment, thus MC concentrations in drinking water might exceed the WHO guideline limit if the treatment efficiency gets compromised. In addition, MC concentrations found in the raw water might represent significant human health risks for people living in areas with only a limited access to the treated or underground drinking water.

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“…As recently reviewed by Bernard et al (2017), ten cyanobacterial genera detected in our study (Tabs. 1-4, Supplementary Tab.…”

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cyanobacteria and microcystin contamination in untreated and treated drinking water in Ghana

Addico¹,

Hardege²,

Kohoutek³

et al. 2017

Adv Ocean Limnol

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“…The occurence of cyanotoxins have been reported in several cyanobacterial genera such as Microcystis, Nodularia, Aphanizomenon, Planktothrix, Anabaena and Cylindrospermopsis (Sivonen et al, 1990;Merel et. al., 2013;Bernard et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

et al. 2017

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Blooms of cyanobacteria are an increasingly frequent phenomenon in freshwater ecosystems worldwide as a result of eutrophication. Many species can produce hepatotoxins that cause severe health hazards to humans. The aim of this study was to identify the bloom forming cyanobacteria species by molecular methods and to amplify genes responsible for hepatotoxin biosynthesis from the environmental samples and isolated strains of cyanobacteria from Küçükçekmece Lagoon, Sapanca, İznik, Manyas and Taşkısı Lakes. A total of 10 bloom samples and 11 isolated strains were examined and Microcystis spp., Planktothrix spp., Nodularia spumigena, Anabaenopsis elenkinii, Sphaerospermopsis aphanizomenoides, Cylindrospermopsis raciborskii were identified. Hepatotoxin genes were detected in 60% of the bloom samples and 45% of the strains. Two Microcystis strains were obtained from Küçükçekmece Lagoon. While the strain assigned to Microcystis flosaquae was non-toxic, Microcystis aeruginosa strain produced microcystin. According to PCR results, the M. aeruginosa and Planktothrix agardhii bloom samples of Küçükçekmece Lagoon contained the microcystin synthetase gene E (mcyE) indicative of microcystin production, however, no microcystin was detected by HPLC. The mcyE gene was also found in Microcystis wesenbergii isolated from Taşkısı Lake, and in all Planktothrix rubescens bloom samples from Sapanca Lake. To our knowledge, this is the first detailed study for identifiying different toxic cyanobacteria species and their hepatotoxin production from several waterbodies in Turkey using molecular methods.

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“…In particular, based on the analysis of isolated strains (Bernard et al, 2016), Aph. flos-aquae and T. bourrellyi were reported as ATX producers (Sivonen et al, 1989;Osswald et al, 2009;Salmaso et al, 2015;2016;Shams et al, 2015), whereas M. aeruginosa and P. rubescens were mainly reported as MC producers (Metcalf and Codd, 2012), and D. lemmermannii as MCs and anatoxin-a(S) producer (Sivonen et al, 1992;Onodera et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

A comparative study of the metabolic profiles of common nuisance cyanobacteria in southern perialpine lakes

Cerasino¹,

Capelli²,

Salmaso³

2017

Adv Ocean Limnol

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This work allowed the comparison of the metabolic profiles of the most important cyanobacteria species in southern perialpine lakes, namely Aphanizomenon flos-aquae, Dolichospermum lemmermannii, Microcystis aeruginosa, Planktothrix rubescens, and Tychonema bourrellyi. Monospecific cultures were obtained from samples of 3 different natural lakes (Garda, Idro, and Caldonazzo). LC-MS/MS analyses were conducted on strains. A first set of experiments was aimed at assessing the presence of the best known toxins (microcystins, nodularins, (homo)anatoxin-a, cylindrospermopsins, paralytic shellfish poisons) in the cultures. Results of this screening study revealed that M. aeruginosa and P. rubescens produced toxic peptides (microcystins), T. bourrellyi produced toxic alkaloids (anatoxin-a and possibly some paralytic shellfish toxins), Aph. flos-aquae and D. lemmermannii did not produce any of the analyzed toxins. M. aeruginosa and P. rubescens showed typical microcystin production with LR form dominant in the former, and RRdm form dominant in the latter. A second set of experiments was aimed at comparing the capability of the 5 cyanobacterial species to produce peptidic secondary metabolites. For this purpose, an untargeted peptidomic analysis was conducted on the strains. The analysis allowed revealing globally 328 metabolites, spanning in a mass range between 400 and 2000 Da. The majority of compounds with masses in the 500-1200 Da range (corresponding to the majority of peptidic secondary metabolites) resulted to be produced by M. aeruginosa and P. rubescens strains, thus indicating a higher ability of these species to produce non-ribosomal peptides compared to the others. 27 metabolites out of 328 could be putatively assigned to specific classes of compounds: microcystins, aeruginosins and anabaenopeptins were the most represented classes of compounds, and were mostly found in M. aeruginosa and P. rubescens strains.

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Appendix 2: Cyanobacteria Associated With the Production of Cyanotoxins

Cited by 57 publications

References 225 publications

Cyanobacteria and microcystin contamination in untreated and treated drinking water in Ghana

Cyanobacteria and microcystin contamination in untreated and treated drinking water in Ghana

Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

A comparative study of the metabolic profiles of common nuisance cyanobacteria in southern perialpine lakes

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