New record of metacercariae of the North American Posthodiplostomum centrarchi (Digenea, Diplostomidae) in pumpkinseed (Lepomis gibbosus) in Hungary

Abstract

Two species of the genus Posthodiplostomum (Digenea: Diplostomatidae) (Posthodiplostomum brevicaudatum Nordmann, 1832 and Posthodiplostomum cuticola Nordmann, 1832) are known as parasites of Hungarian native fishes. Metacercariae of P. cuticola are widespread in Europe and cause black spot disease. Several species of Posthodiplostomum were described also from North America but none of them has been isolated in Hungary up to now. Posthodiplostomum centrarchi Hoffman, 1958 has been detected recently in pumpkinseeds (Lepomis gibbosus L., 1758) in several European countries. Posthodiplostomum centrarchi was isolated for the first time in Hungary from pumpkinseeds caught in the Maconka water reservoir in 2015. Thereafter, several natural waters (e.g. the River Danube, Lake Balaton and the Sió channel) were sampled in order to determine its presence and distribution. Only the native species P. cuticola was detected in Lake Balaton on cyprinids but a relatively high infection rate of P. centrarchi was observed in the Sió channel close to the lake. Pathological changes were absent, and metacercariae were mostly attached to the surface of the liver, kidney and heart. The phylogenetic analysis of the ITS and COI sequences of P. centrarchi and P. cuticola clustered into two distinct branches, which was in agreement with the morphological results.

Unravelling the diversity of the Crassiphialinae (Digenea: Diplostomidae) with molecular phylogeny and descriptions of five new species

“…cox 1; e.g. Hernández-Mena et al., 2017 ; López-Hernández et al., 2018 ; Hoogendoorn et al., 2019 ; Achatz et al., 2019a , c , 2020a ; Cech et al, 2020 ; Tkach et al., 2020 ) often produce topologies which are much less resolved than those based on slower mutating genes such as 28S (e.g. Hernández-Mena et al., 2017 ; Hoogendoorn et al., 2019 ; Achatz et al., 2019a , c , 2020a ; Sokolov and Gordeev, 2020 ; Tkach et al., 2020 ).…”

“…are commonly collected and studied using molecular tools (e.g. Locke et al, 2010 ; Blasco-Costa and Locke, 2017 ; Kvach et al, 2017 ; Stoyanov et al, 2017 ; Locke et al, 2018 ; López-Hernández et al, 2018 ; Cech et al, 2020 ), few studies which produced DNA sequences have provided species identifications based on adult morphology (e.g. Locke et al, 2018 ).…”

First integrative study of the diversity and specificity of metacercariae of Posthodiplostomum Dubois, 1936 from native and introduced fishes in the Caribbean

“…minimum have been recovered only from leuciscid hosts (Locke et al, 2010;Komatsu et al, 2020;Achatz et al, 2021), and those of Po. centrarchi nearly exclusively from Lepomis spp., which mirrors the specificity of cercariae in Hoffman's (1958) Locke et al, 2010Locke et al, , 2018Kvach et al, 2017;Stoyanov et al, 2017;Boone et al, 2018;Stoyanov et al, 2018;Cech et al, 2020;Komatsu et al, 2020) León et al (2022). While morphological comparisons do not support conspecificity, the differences observed (see Remarks) may be to some extent related to geographic (Stoyanov et al, 2017) and host-associated (Palmieri, 1975(Palmieri, , 1977a(Palmieri, , 1977b(Palmieri, , 1977c (Locke et al, 2010), 1.7% (Stoyanov et al, 2017), 3.91% (Boone et al, 2018) and 3.8% (Achatz et al, 2021)] and other digeneans (e.g.…”

“…In addition, care is needed in interpreting host ranges of lineages in Pérez-Ponce de León et al (2022) due to conflicts between the paper and GenBank records. For example, OK315743 and OK314903 appear to be sequences from the same individual worm (see Locke et al, 2010Locke et al, , 2018Kvach et al, 2017;Stoyanov et al, 2017;Boone et al, 2018;Stoyanov et al, 2018;Cech et al, 2020;Komatsu et al, 2020;Achatz et al, 2021) The modern world provides species of Posthodiplostomum with many opportunities to colonize newly encountered host taxa, by reshuffling host assemblages through changing climate (Marcogliese, 2001) and species introductions (Rahel, 2010). In Puerto Rico, the accidental or intentional introduction of non-native fish species has occurred since the early 1900s (Neal et al, 2004, cited in Neal et al, 2009.…”

“…8 and Po. centrarchi encounter in diverse and widespread habitats (present study; Locke et al ., 2010, 2018; Kvach et al ., 2017; Stoyanov et al ., 2017; Boone et al ., 2018; Stoyanov et al ., 2018; Cech et al ., 2020; Komatsu et al ., 2020). Our data from Caribbean hosts and parasites not previously surveyed are thus consistent with both these particular patterns and the host specificity that generally prevails among this group of parasites.…”