New procedures to freeze donkey semen and its influence on mares and jennies fertility

Oliveira, José Victor de; Papa, Frederico Ozanam; Melo-Ona, C. M.; Monteiro, Gabriel Augusto; Filho, José Nicolau Próspero Puoli; Alvarenga, Marco Antônio

doi:10.1016/j.jevs.2012.06.075

Cited by 11 publications

(11 citation statements)

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“…; Oliveira et al. ) and were lower (26% and 45%) when cooled semen was used (Alvarez et al. ; Vidament et al.…”

Section: Introductionmentioning

confidence: 99%

“…; Loomis ; Vidament ), even when sperm are deposited deep into the uterine horn (26%) (Oliveira et al. ).…”

Section: Introductionmentioning

confidence: 99%

“…Pregnancy rates obtained by artificial insemination (AI) using fresh semen in the donkey ranged 51-73% (Marianelli et al 2009;Oliveira et al 2012) and were lower (26% and 45%) when cooled semen was used (Alvarez et al 2004;Vidament et al 2009), even though preservation time was short (12 and 8 h, respectively). Pregnancy rates using frozen semen are lower in donkeys (0-36%) (Trimeche et al 1998;Oliveira et al 2006;Vidament et al 2009;Rota et al 2012), compared to the horse (38-52%) (Barbacini et al 1999;Loomis 2001;Vidament 2005), even when sperm are deposited deep into the uterine horn (26%) (Oliveira et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Post‐Thaw Addition of Seminal Plasma on Motility, Viability and Chromatin Integrity of Cryopreserved Donkey Jack (Equus asinus) Spermatozoa

Sabatini

Mari

Mislei

et al. 2014

Reprod Domestic Animals

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Pregnancy rates in donkeys after artificial insemination with cryopreserved semen are still low, compared to the horse species. Addition of autologous seminal plasma to frozen-thawed semen appeared to improve pregnancy rates. The aims of this study were to evaluate (1) sperm motility and plasma membrane integrity after thawing (T0) and after one and 2 h (T1 and T2) of post-thaw incubation in either 0% (SP0) or 70% (SP70) autologous seminal plasma and (2) sperm motility, plasma membrane integrity and DNA quality (%COMP-αt) after thawing (T0) and after 2 and 4 h (T2 and T4) of post-thaw incubation in either 0% (SP0), 5% (SP5) or 20% (SP20) homologous seminal plasma. In experiment 1, seminal plasma decreased total and progressive sperm motility and plasma membrane intact spermatozoa immediately after dilution and at all following time points (p < 0.05). In experiment 2, total and progressive motility did not differ between treatments immediately after dilution and between SP0 and SP5 at T2, while they were lower in both SP5 and SP20 than in SP0 at T4. Plasma membrane intact sperm cells did not differ between SP0 and SP5 and were lower in SP20 at all time points. DNA quality was not affected by treatment immediately after dilution and was significantly worse for SP20 after 4 h of incubation (p < 0.05). The post-thaw addition of seminal plasma at the tested concentrations did not improve donkey frozen semen characteristics in vitro over time.

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“…; Oliveira et al. ) and were lower (26% and 45%) when cooled semen was used (Alvarez et al. ; Vidament et al.…”

Section: Introductionmentioning

confidence: 99%

“…; Loomis ; Vidament ), even when sperm are deposited deep into the uterine horn (26%) (Oliveira et al. ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Post‐Thaw Addition of Seminal Plasma on Motility, Viability and Chromatin Integrity of Cryopreserved Donkey Jack (Equus asinus) Spermatozoa

Sabatini

Mari

Mislei

et al. 2014

Reprod Domestic Animals

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“…Donkeys have been gaining in importance as companion, pack and draft animals in some countries; the artificial insemination protocols followed with these animals are usually those designed for mares, with disappointing results [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, jenny has all the vulva under the pelvis floor and higher vaginal vestibule inclination than mare [ 17 , 18 , 19 ]. Furthermore, jennies have a relatively longer cervix with more tortuous folds [ 18 , 27 , 28 ] that protrudes into the vagina making artificial insemination more difficult and the ability to conceive after insemination poorer than in mares [ 11 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Endometrial Cytology During the Different Phases of the Estrous Cycle in Jennies: New Evidences

Quartuccio

Cristarella

Medica

et al. 2020

Animals

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Since in the mare and other animal species such as bitches and cats, the endometrial cell pattern varies depending on the phase of the estrous cycle, the aim of this study was to describe and quantify the endometrial cytological (EC) findings in cycling jennies. EC of eight nonpregnant jennies by cytobrush (CB) at diestrus (day 1 and day 14) and estrous (day 21) were evaluated. All slides were stained with Wright´s stain and microscopically examined at both 400× and 1000× magnification. Seven high-power fields (400×) were assessed in each smear and the endometrial epithelial cells and neutrophils (PMNs) were counted. Endometrial epithelial cells were classified as intact, distorted or fragmented and, on the basis of the presence of dense groups, in monolayer or single clusters. Cytoplasmic characteristics, such as vacuolation or streaming and size, form, position of nuclear characteristics, including karyorrhexis, were recorded. Background aspect, as clear, proteinaceous, or debris, was also considered. In general, sampling by CB provided a yield of cells and clumped endometrial epithelial cells in many smears, being more abundant in estrus than early and late diestrus. Individual endometrial epithelial cells, during estrous, presented a columnar morphology, ciliated or not ciliated and basal nuclei. During diestrus phase, endometrial epithelial cells presented a more cuboidal ciliated or not ciliated morphology. Moderate amount of proteinacious material and red blood cells (RBC) was also observed. Non variation in the percentage of PMNs during diestrus was obtained, but lower and segmented PMNs in CB smears were shown in estrous. This study provides new insights on the physiological changes of endometrial epithelial cells in cycling jennies during the estrus cycle. The CB technique represents a suitable and adequate method for endometrial evaluation, taking into account cytological and/or cytopathological purposes also in jennies.

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Influence of seminal plasma on leucocyte migration and amount of COX-2 protein in the jenny endometrium after insemination with frozen–thawed semen

Vilés

Rabanal

Rodríguez-Prado

et al. 2013

Animal Reproduction Science

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New procedures to freeze donkey semen and its influence on mares and jennies fertility

Cited by 11 publications

References 1 publication

Effect of Post‐Thaw Addition of Seminal Plasma on Motility, Viability and Chromatin Integrity of Cryopreserved Donkey Jack (Equus asinus) Spermatozoa

Effect of Post‐Thaw Addition of Seminal Plasma on Motility, Viability and Chromatin Integrity of Cryopreserved Donkey Jack (Equus asinus) Spermatozoa

Endometrial Cytology During the Different Phases of the Estrous Cycle in Jennies: New Evidences

Influence of seminal plasma on leucocyte migration and amount of COX-2 protein in the jenny endometrium after insemination with frozen–thawed semen

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