Endometrial Cytology During the Different Phases of the Estrous Cycle in Jennies: New Evidences

Abstract

Since in the mare and other animal species such as bitches and cats, the endometrial cell pattern varies depending on the phase of the estrous cycle, the aim of this study was to describe and quantify the endometrial cytological (EC) findings in cycling jennies. EC of eight nonpregnant jennies by cytobrush (CB) at diestrus (day 1 and day 14) and estrous (day 21) were evaluated. All slides were stained with Wright´s stain and microscopically examined at both 400× and 1000× magnification. Seven high-power fields (400×) were assessed in each smear and the endometrial epithelial cells and neutrophils (PMNs) were counted. Endometrial epithelial cells were classified as intact, distorted or fragmented and, on the basis of the presence of dense groups, in monolayer or single clusters. Cytoplasmic characteristics, such as vacuolation or streaming and size, form, position of nuclear characteristics, including karyorrhexis, were recorded. Background aspect, as clear, proteinaceous, or debris, was also considered. In general, sampling by CB provided a yield of cells and clumped endometrial epithelial cells in many smears, being more abundant in estrus than early and late diestrus. Individual endometrial epithelial cells, during estrous, presented a columnar morphology, ciliated or not ciliated and basal nuclei. During diestrus phase, endometrial epithelial cells presented a more cuboidal ciliated or not ciliated morphology. Moderate amount of proteinacious material and red blood cells (RBC) was also observed. Non variation in the percentage of PMNs during diestrus was obtained, but lower and segmented PMNs in CB smears were shown in estrous. This study provides new insights on the physiological changes of endometrial epithelial cells in cycling jennies during the estrus cycle. The CB technique represents a suitable and adequate method for endometrial evaluation, taking into account cytological and/or cytopathological purposes also in jennies.

Clinical haematology, biochemistry profiles and erythrocytic morphometry of confiscated Sunda pangolins (Manis javanica) in the Centre for Rehabilitation and Quarantine of Confiscated Animals, Wildlife and Alien Species, Thailand

“…In addition, increased lymphocyte number might be another indicator of body need for interacting with injuries. The lower neutrophils in this study may also involve the oestrous cycle causing an increased neutrophil immigration into the reproductive tract (Hussain and Daniel, 1991;Williams et al, 1992;Quartuccio et al, 2020).…”

Use of the endometrial histopathology to improve diagnosis of donkeys with endometritis

“…In the endometrial cytology of the jennies, the impact of oestrus was mainly on the changes in cell morphology. The endometrial epithelial cells tend to be cuboidal in dioestrus, while columnar in oestrus (Quartuccio et al, 2020). In the histopathologic findings of this study, the oestrus cycle mainly affected the morphology of the endometrial stroma and glands.…”

Edible bird’s nest protects histomorphology of rat’s uterus against cadmium (Cd) toxicity through a reduction of Cd deposition and enhanced antioxidant activity

“…This observation is in accordance with the study carried out by Nna et al Neutrophil infiltration in the uterus was mild in all groups due to a normal physiological process in the estrus stage. During estrus evaluation, segmental neutrophils and erythrocytes are common responses ( Quartuccio et al, 2020 ). However, scores for infiltration of inflammatory cells, congestion, degeneration of LE, and GE were significantly higher in the Cd-only treated group.…”