New polyhydroxylated sterols from Palythoa tuberculosa and their apoptotic activity in cancer cells

Elbagory, Abdulrahman M.; Meyer, Mervin; Ali, Abdel-Hamid A. M.; Ameer, Farouk; Parker-Nance, Shirley; Benito, Maria Teresa; Doyagüez, Elisa G.; Jimeno, M. Luísa; Hussein, Ahmed A.

doi:10.1016/j.steroids.2015.06.009

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…Algae-derived compounds possess various health benefits (Mayer and Hamann 2003;Athukorala and Jeon 2005;Heo et al 2005;Kotake-Nara et al 2005). The sterols including cholesterol, β-sitosterol, campesterol, and polyhydroxylated sterols isolated from algae were shown to strong bioactivities, especially anticancer and anti-inflammatory activities (Kazłowska et al 2013;Elbagory et al 2015). The freshwater green alga, Spirogyra sp.…”

Section: Sterol Content Of Spe and Its Fractionsmentioning

confidence: 99%

In vitro and in vivo anti-inflammatory activities of a sterol-enriched fraction from freshwater green alga, Spirogyra sp.

2020

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Background Inflammation plays a crucial role in the pathogenesis of many diseases such as arthritis and atherosclerosis. In the present study, we evaluated anti-inflammatory activity of sterol-rich fraction prepared from Spirogyra sp., a freshwater green alga, in an effort to find bioactive extracts derived from natural sources. Methods The sterol content of ethanol extract of Spirogyra sp. (SPE) was enriched by fractionation with hexane (SPEH), resulting 6.7 times higher than SPE. Using this fraction, the in vitro and in vivo anti-inflammatory activities were evaluated in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells and zebrafish. Results SPEH effectively and dose-dependently decreased the production of nitric oxide (NO) and prostaglandin E2 (PGE2). SPEH suppressed the production of pro-inflammatory cytokines including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and IL-1β through downregulating nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW 264.7 cells without cytotoxicity. The in vivo test results indicated that SPEH significantly and dose-dependently reduced reactive oxygen species (ROS) generation, cell death, and NO production in LPS-stimulated zebrafish. Conclusions These results demonstrate that SPEH possesses strong in vitro and in vivo anti-inflammatory activities and has the potential to be used as healthcare or pharmaceutical material for the treatment of inflammatory diseases.

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Section: Sterol Content Of Spe and Its Fractionsmentioning

confidence: 99%

In vitro and in vivo anti-inflammatory activities of a sterol-enriched fraction from freshwater green alga, Spirogyra sp.

2020

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“…Compound 1, obtained as an amorphous powder, had the molecular formula C 30 H 48 O 4 established by HRESIMS {m/z 473.6605 (M + +1), Furthermore, the NMR spectral data of 1 were remarkably similar to those for compound 2 [15] with the only difference being the presence of an additional acetoxy group in 1. The large deshielding of H-1 at δ 5.66 vs that of δ 4.21 for compound 2 [15] suggested that the acetoxy group was situated at C-1, while the multiplet centered at δ 3.92 had a similar complexity normally seen for a 3 βcarbinol proton in a steroid skeleton [16]. The oxygenated methine signals at δ C 78.3 (C-1) 66.7 (C-3) and 68.9 (C-11) are in similar positions as the 1α,3β,11α-triol system in the known compound 2.…”

mentioning

confidence: 79%

Polyhydroxy Sterols Isolated from the Red Sea Soft Coral Lobophytum crassum and their Cytotoxic Activity

Aboutabl

Selim

Azzam

et al. 2017

Natural Product Communications

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One new (1) together with four known sterols (2-5) and a sesquiterpene (6) were isolated from a polar extract of the Red Sea soft coral Lobophytum crassum. The compounds were identified as 24-methylenecholest-5-ene-1α,3β,11α-triol 1-acetate (1), 24-methylenecholest-5-ene-1α,3β,11α-triol (2), 24methylenecholest-5-ene-3β-ol (3), 24-methylenecholestane-1α,3β,5,6,11α-pentol (4), 24-methylenecholestane-3β,5,6-triol (5) and alismoxide (6) based on extensive NMR analysis. The cytotoxicity of compounds 1-6 was evaluated in vitro using three human cancer cell lines viz., HepG2, Hep-2 and HCT-116. Compound 1 showed selective cytotoxic activity against HepG2, while 3 exhibited cytotoxicity against all tested cell lines.

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“…Zoanthids of the genus Palythoa inhabit the littoral zone around the globe [1]. At least 110 Palythoa species are cited in the literature.…”

Section: Introductionmentioning

confidence: 99%

A Novel Phospholipase A2 Isolated from Palythoa caribaeorum Possesses Neurotoxic Activity

Cuevas-Cruz

Lazcano-Pérez

Hernández-Guzmán

et al. 2019

Toxins

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Zoanthids of the genus Palythoa are distributed worldwide in shallow waters around coral reefs. Like all cnidarians, they possess nematocysts that contain a large diversity of toxins that paralyze their prey. This work was aimed at isolating and functionally characterizing a cnidarian neurotoxic phospholipase named A2-PLTX-Pcb1a for the first time. This phospholipase was isolated from the venomous extract of the zoanthid Palythoa caribaeorum. This enzyme, which is Ca2+-dependent, is a 149 amino acid residue protein. The analysis of the A2-PLTX-Pcb1a sequence showed neurotoxic domain similitude with other neurotoxic sPLA2´s, but a different catalytic histidine domain. This is remarkable, since A2-PLTX-Pcb1a displays properties like those of other known PLA2 enzymes.

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New polyhydroxylated sterols from Palythoa tuberculosa and their apoptotic activity in cancer cells

Cited by 8 publications

References 27 publications

In vitro and in vivo anti-inflammatory activities of a sterol-enriched fraction from freshwater green alga, Spirogyra sp.

In vitro and in vivo anti-inflammatory activities of a sterol-enriched fraction from freshwater green alga, Spirogyra sp.

Polyhydroxy Sterols Isolated from the Red Sea Soft Coral Lobophytum crassum and their Cytotoxic Activity

A Novel Phospholipase A2 Isolated from Palythoa caribaeorum Possesses Neurotoxic Activity

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