New Physiological Importance of Two Classic Residual Products: Carbon Monoxide and Bilirubin

Gîlcă, Marilena

doi:10.1006/bmme.1997.2610

Cited by 69 publications

(52 citation statements)

References 37 publications

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“…Recent studies have shown that bilirubin should not be considered as just a metabolic waste product. It functions in-vivo as a powerful anti-oxidant, anti-mutagen, anti-complement and an endogenous tissue protector (13).…”

Section: Bilirubin As An Anti-oxidantmentioning

confidence: 99%

Serum adenosine deaminase, 5′ nucleotidase and malondialdehyde in acute infective hepatitis

Pratibha

Anand

Agarwal

2004

Indian J Clin Biochem

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Serum adenosine deaminase (ADA), 5'nucleotidase (5'NT) and malondialdehyde (MDA) were estimated in patients with acute infective hepatitis (AIH) along with the routine parameters of liver disease.Present study is done to evaluate these special parameters in patients with clinical history of AIH and to assess the utility of these parameters as diagnostic/ prognostic indices of liver function and to correlate special parameters with routine liver function tests (LFT). ADA, 5'NT and MDA along with routine LFT was estimated in 25 patients with AIH and 25 samples from healthy voluntary blood donars served as the control group. Routine LFT was estimated by standard clinical chemistry procedures on dade behring analyser and ADA, 5'NT and MDA were estimated by berthlot reaction, fiske and subbarao method and thiobarbituric acid method respectively.Statistical analysis showed that serum ADA, 5'NT and MDA were significantly higher in patients as compared with the controls. There was a significant positive correlation between ADA and total bilirubin and MDA and total bilirubin. Hence we can conclude that these tests would be more sensitive to diagnose the patients with AIH and that the raised bilirubin levels could be looked upon, as a protective mechanism which the liver has evolved in order to combat oxidative stress. KEY WORDSADA, 5' NT, MDA, AIH.

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Section: Bilirubin As An Anti-oxidantmentioning

confidence: 99%

Serum adenosine deaminase, 5′ nucleotidase and malondialdehyde in acute infective hepatitis

Pratibha

Anand

Agarwal

2004

Indian J Clin Biochem

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Section: Introductionmentioning

confidence: 99%

“…The existence of endogenous CO in the human body arises from heme catabolism (Meredith and Vale 1988;Ernst and Zibrak 1998) and oxidation of organic molecules (Marilena 1997). Endogenous CO acts as a neurotransmitter for long-term potentiation, consequently playing a key role in memory and learning (Marilena 1997). It also plays a role in modulating inflammation, apoptosis, cell proliferation, mitochondrial biogenesis (Weaver 2009) and vascular relaxation (Marilena 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Endogenous CO acts as a neurotransmitter for long-term potentiation, consequently playing a key role in memory and learning (Marilena 1997). It also plays a role in modulating inflammation, apoptosis, cell proliferation, mitochondrial biogenesis (Weaver 2009) and vascular relaxation (Marilena 1997). Exogenous sources of CO intoxication include smoking, forest fires, pollutants, and improper usage of heaters or furnaces (Weaver 2009;Kumar, Prakash et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Neuroimaging Studies in Carbon Monoxide Intoxication

Chang¹,

Chang²,

Huang³

et al. 2012

Neuroimaging - Cognitive and Clinical Neuroscience

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“…HO activity is critical for maintaining the ferric ion cycle and provides iron required by the human body (2). Biliverdin and bilirubin are two well known antioxidants (3,4). Although there is controversy regarding the biological function of CO (5), CO is implicated in neural conduction (6,7), protection of hemorrhagic shock (8), and regulation of microvascular contractility (9).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the crystal structure and function to wild-type and His25Ala mutant human heme oxygenase-1

Zhou

Zhong²,

Zhang³

et al. 2009

Int J Mol Med

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Abstract. Human heme oxygenase-1 (hHO-1) is a rate-limiting enzyme in heme metabolism. It regulates serum bilirubin level. Site-directed mutagenesis studies indicate that the proximal residue histidine 25 (His25) plays a key role in hHO-1 activity. A highly purified hHO-1 His25Ala mutant was generated and crystallized with a new expression system. The crystal structure of the mutant was determined by X-ray diffraction technology and molecular replacement at the resolution of 2.8 Å, and the model of hHO-1 His25Ala mutant was refined. The final crystallographic and free R factors were 0.245 and 0.283, respectively. The standard bond length deviation was 0.007 Å, and the standard bond angle deviation was 1.3˚. The mutation of His25 to Ala led to an empty pocket underneath the ferric ion in the heme, leading to loss of binding iron ligand. Although this did not cause an overall structural change, the enzymatic activity of the mutant hHO-1 was reduced by 90%. By supplementing imidazole, the HO-1 activity was restored 90% to its normal level. These data suggest that Ala25 remains unchanged in the structure compared to His25, but the important catalytic function of hHO-1 is lost. Thus, it appears that His25 is a crucial residue for proper hHO-1 catalysis.

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New Physiological Importance of Two Classic Residual Products: Carbon Monoxide and Bilirubin

Cited by 69 publications

References 37 publications

Serum adenosine deaminase, 5′ nucleotidase and malondialdehyde in acute infective hepatitis

Serum adenosine deaminase, 5′ nucleotidase and malondialdehyde in acute infective hepatitis

Neuroimaging Studies in Carbon Monoxide Intoxication

Comparison of the crystal structure and function to wild-type and His25Ala mutant human heme oxygenase-1

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