Serum adenosine deaminase, 5′ nucleotidase and malondialdehyde in acute infective hepatitis

Pratibha, K.; Anand, Usha; Agarwal, Rajni

doi:10.1007/bf02894271

Cited by 28 publications

(25 citation statements)

References 7 publications

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“…Increased plasma bilirubin level in PEMF-exposed group could also be a compensatory/retaliatory phenomenon in response to cellular peroxidative changes. This is because bilirubin functions as a powerful antioxidant, anti-mutagen, and an endogenous tissue protector in vivo [23]. Thus, there is a positive correlation between MDA content and plasma bilirubin level, which supports the study findings of Pratibha et al [23].…”

Section: Discussionsupporting

confidence: 81%

“…This is because bilirubin functions as a powerful antioxidant, anti-mutagen, and an endogenous tissue protector in vivo [23]. Thus, there is a positive correlation between MDA content and plasma bilirubin level, which supports the study findings of Pratibha et al [23]. On the contrary, another group of authors did not observe any significant modification in plasma bilirubin level in rats under the effect of long-term PEMF.…”

Section: Discussionsupporting

confidence: 71%

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Oxidative Stress and Apoptosis in Relation to Exposure to Magnetic Field

Emre

Çetiner

Zencir

et al. 2010

Cell Biochem Biophys

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We investigated the effect of extremely low-frequency electromagnetic field (ELF-EMF) with pulse trains exposure on lipid peroxidation, and, hence, oxidative stress in the rat liver tissue. The parameters that we measured were the levels of plasma alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase as well as plasma albumin, bilirubin, and total protein levels in 30 adult male Wistar rats exposed to ELF. We also determined the percentage of apoptotic and necrotic cells of the kidney extracts from the animals by flow cytometry method. Apoptotic cell death was further characterized by monitoring DNA degradation using gel electrophoresis. The results showed an increase in the levels of oxidative stress indicators, and the flow cytometric data suggested a possible relationship between the exposure to magnetic field and the cell death. We showed significantly lower necrotic cell percentages in experimental animals compared to either unexposed or sham control groups. However, DNA ladder analyses did not differentiate between the groups. Our results were discussed in relation to the response of biological systems to EMF.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 71%

Oxidative Stress and Apoptosis in Relation to Exposure to Magnetic Field

Emre

Çetiner

Zencir

et al. 2010

Cell Biochem Biophys

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“…Significantly elevated adenosine deaminase activity was reported in cerebral cortex, cerebellum and brain stem of rats due to acute ethanol toxicity [41]. Pradipha et al [39] reported elevated MDA and ADA levels in acute infective hepatitis and also showed that lipid peroxidation is associated with loss of membrane integrity which subsequently causes the release of membrane-bound enzymes including ADA and 5' nucleotidase. In our study, we found elevated ADA activities in synaptosomal fractions of rat forebrains exposed to chronic ethanol administration.…”

Section: Resultsmentioning

confidence: 97%

“…It's an important enzyme for purine metabolism, converting nucleosides adenosine and 2'-deoxyadenosine into inosine and 2'-deoxyinosine, respectively [38]. ADA is considered as a membrane-bound enzyme [39]. Elevated activities of 5'-nucleotidase and adenosine deaminase were reported in rat myocardium exposed to ethanol without any significant change in the activities of gammaglutamyl transpeptidase and AMP deaminase [40].…”

Section: Resultsmentioning

confidence: 99%

Effects of Chronic Ethanol Consumption on Brain Synaptosomes and Protective Role of Betaine

et al. 2007

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To evaluate the cytotoxic effects of chronic ethanol consumption on brain cerebral synaptosomes and preventive role of betaine as a methyl donor and S-adenosylmethionine precursor, 24 male Wistar rats were divided into three groups: control, ethanol (8 g/kg/day) and ethanol plus betaine(0.5% w/v) group. Animals were fed 60 ml/diet per day for two months, then sacrificed. Malondialdehyde (MDA), protein carbonyl contents and adenosine deaminase (ADA) activities were determined in synaptosomal/mitochondrial enriched fraction isolated from rat cerebral cortexes. When compared to controls, ethanol containing diet significantly increased MDA levels (P < 0.05), also increased protein carbonyl levels and adenosine deaminase activities. But these were not statistically significant (P > 0.05). However, adding betaine to ethanol containing diet caused a significant decrease in MDA, protein carbonyl levels and adenosine deaminase activities (P < 0.05). These results indicate that betaine may appear as a protective nutritional agent against cytotoxic brain damage induced by chronic ethanol consumption.

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“…All these processes culminate in an increase in serum MDA level. Hence increase serum MDA could be used as a marker for free radical mediated destruction of liver parenchymal cells [45]. Thus chronic exposure of lead in battery manufacturing workers of Western Maharashtra (India) clearly showed an adverse effect on heme biosynthesis and decrease activities of SOD and catalase in associated with increased lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lead (Pb) Exposure on the Activity of Superoxide Dismutase and Catalase in Battery Manufacturing Workers (BMW) of Western Maharashtra (India) with Reference to Heme biosynthesis

Bhagwat¹,

Patil

Dongre

et al. 2006

IJERPH

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Abstract:The aim of this study was to estimate the activity of superoxide dismutase (SOD) and catalase in erythrocytes and malondialdehyde (MDA) in plasma of battery manufacturing workers (BMW) of Western Maharashtra (India) who were occupationally exposed to lead (Pb) over a long period of time (about 15 years). This study was also aimed to determine the Pb intoxication resulted in a disturbance of heme biosynthesis in BMW group. The blood Pb level of BMW group (n = 28) was found to be in the range of 25.8 -78.0 µg/dL (mean + SD, 53.63 + 16.98) whereas in Pb unexposed control group (n = 35) the range was 2.8 -22.0 µg/dL (mean + SD, 12.52 + 4.08). The blood level (Pb-B) and urinary lead level (Pb-U) were significantly increased in BMW group as compared to unexposed control. Though activated d-aminolevulinic acid dehydratase (ALAD) activities in BMW group did not show any significant change when compared to control group but activated / non activated erythrocyte -ALAD activities in BMW group showed a significant increase. Erythrocyte-zinc protoporphyrin (ZPP), urinary daminolevulinic acid (ALA-U) and porphobilinogen (PBG-U) of BMW groups elevated significantly as compared to control. A positive correlation (r = 0.66, p< 0.001) between Pb-B and ALA-U were found in BMW group but no such significant correlation (r = 0.02, p> 1.0) were observed in control group. Hematological study revealed a significant decrease of hemoglobin concentration, packed cell volume (%) and other blood indices and a significant increase of total leucocytes count in BMW group in comparison to control group. The serum MDA content was significantly increased (p< 0.001) and the activities of antioxidant enzymes such as erythrocyte-SOD (p< 0.001) and erythrocytecatalase (p< 0.001) were significantly reduced in BMW group as compared to control group. A positive correlation (r = 0.45, p<0.02) between Pb-B and serum MDA level was observed in BMW group (Pb-B range 25.8 -78.0 µg / dL) but such significant correlation did not notice in control group (Pb-B range 2.8 -22.0 µg / dL).The study clearly showed an adverse effect of heme biosynthesis and imbalance of pro-oxidant / antioxidant status in lead exposed battery manufacturing workers resulting in increase in lipid peroxidation associated with decrease in erythrocyte-SOD and erythrocyte-catalase activities.

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Serum adenosine deaminase, 5′ nucleotidase and malondialdehyde in acute infective hepatitis

Cited by 28 publications

References 7 publications

Oxidative Stress and Apoptosis in Relation to Exposure to Magnetic Field

Oxidative Stress and Apoptosis in Relation to Exposure to Magnetic Field

Effects of Chronic Ethanol Consumption on Brain Synaptosomes and Protective Role of Betaine

Effect of Lead (Pb) Exposure on the Activity of Superoxide Dismutase and Catalase in Battery Manufacturing Workers (BMW) of Western Maharashtra (India) with Reference to Heme biosynthesis

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