New peptidendrocins and anticancer chartreusin from an endophytic bacterium of Dendrobium officinale

Zhao, Huimin; Chen, Xiabin; Chen, Xiaoling; Zhu, Youjuan; Kong, Yichao; Zhang, Sifang; Deng, Xingyu; Ouyang, Pengfei; Zhang, Wei; Hou, Shurong; Wang, Xiachang; Xie, Tian

doi:10.21037/atm.2020.03.227

Cited by 18 publications

(9 citation statements)

References 28 publications

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“…They mainly focused on the isolation, identification and diversity of endophytes, the screening of growth‐promoting microbes, and the separation, purification and activity study of the secondary metabolites of endophytes. Considering that D. officinale has anti‐tumour property and improves immunity and other pharmacological activities, the functional value of its endophytic metabolites is also a hot spot in recent research on D. officinale (Zeng, Yang, Wang, et al, 2019; Zeng, Yang, Zong, et al, 2019; Zhao et al, 2020). There are a few reports on the rhizospheric microorganisms of D. officinale .…”

Section: Discussionmentioning

confidence: 99%

Taxonomic and functional diversity of Dendrobium officinale microbiome in Danxia habitat

Wang

Liang

et al. 2022

Journal of Applied Microbiology

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Aims Microbial communities that inhabit plants are crucial for plant survival and well‐being including growth in stressful environments. The medicinal plant, Dendrobium officinale grows in the barren soils of the Danxia Habitat. However, the microbiome composition and functional potential for growth of this plant in this environment are still unexplored. Methods and Results In this study, we analysed the taxonomic and functional diversity of the D. officinale Microbiome by metagenomic sequencing of both rhizosphere and endosphere samples. A total of 155 phyla, 122 classes, 271 orders, 620 families and 2194 genera were identified from all samples. The rhizospheric microbes (DXRh) were mainly composed of Proteobacteria and Acidobacteria, while Basidiomycota and Ascomycota were the most dominant phyla in root endosphere (DXRo) and stem endosphere (DXS), respectively. Most of the dominant microbial communities had been reported to have diverse functional potentials that can help plant growth and development in stressful and nutrient‐deprived ecological environmental. These include plant growth promoting rhizobacteria (PGPR) such as Massilia, Pseudomonas, Bradyrhizobium, Klebsiella, Streptomyces, Leclercia, Paenibacillus, Frankia and Enterobacter in the DXRh, Tulasnella and Serendipita in the DXRo, Colletotrichum and Burkholderia in the DXS and Paraburkholderia, Rhizophagus and Acetobacter in endosphere. Analysis using the KEGG, eggNOG and CAZy databases showed that metabolic pathways such as carbohydrate metabolism, amino acid metabolism, energy metabolism, genetic information processing and environmental information processing are significantly abundant, which may be related to the survival, growth and development of D. officinale in a stressful environment. Conclusions We speculated that the microbial community with diverse taxonomic structures and metabolic functions inhabiting in different niches of plants supports the survival and growth of D. officinale in the stressful environment of Danxia Habitat. Significance and Impact of the Study This study provided an important data resource for microbes associated with D. officinale and theoretical foundation for further studies.

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Section: Discussionmentioning

confidence: 99%

Taxonomic and functional diversity of Dendrobium officinale microbiome in Danxia habitat

Wang

Liang

et al. 2022

Journal of Applied Microbiology

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“…D. o cinale, a traditional Chinese functional food and perennial medicinal herb, has shown great pharmacological activity and economic value in recent years [1,14] . Due to low natural regeneration rates, habitat destruction, excessive collection and commercial trade, wild resources are on the verge of extinction and cannot meet the growing market demand, so massive commercial arti cially protected cultivation has been used to expand production [12] .…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Nutritional Quality of Dendrobium Officinale Kimura Et Migo After Transplantation of Artificially Cultivated Plants From South to North China During Different Growing Years

Guo

Zhang

Yan

et al. 2021

Preprint

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Background: Dendrobium officinale Kimura et Migo is a traditional functional food and medicinal plant in China. Due to low natural regeneration rates, habitat destruction, excessive collection and commercial trade, D. officinale is severely threatened, and commercial artificial-sheltered cultivation has been massively used to meet the needs of the market. Aim: To comprehensively compare the accumulation of nutritional compounds during 3-5 years of introduced, artificial-sheltered cultivation from southern to northern China.Methods: D. officinale of the same species were artificially cultivated in the southern traditional cultivation area, Anhui and the new northern cultivation area, Beijing. First, samples were collected in the third, fourth, and fifth years of growth, and nutritional quality indexes, including polysaccharides, alkaloids, flavonoids and total phenolic content, were determined. Second, an untargeted metabolomics method was used to investigate metabolic variations in D. officinale stems between Anhui and Beijing cultures in the fifth year.Results: After comparing the nutrient accumulation in different growing years, the idea harvest time was found in the third growing year in both cultivation areas. Of them, the contents of polysaccharides, flavonoids and total polyphenol were higher in cultivation in Anhui than Beijing, but the accumulation of alkaloid content was much lower in Anhui. The highest amount of polysaccharides of Dendrobium officinale was found in the three-year cultivation in Anhui, which reached 515.75 mg/g. When metabolites were analysed, a total of 272 metabolites were detected in the current study, including 27 up-regulated and 73 down-regulated metabolites in D. officinale cultivated in Beijing compared with samples from Anhui. Conclusion: D. officinale artificially and transplanted cultivated from southern to northern China showed some significant differences in the accumulation of nutrient compounds. Planting in northern China has some specific advantages, but the overall nutritional value is not as good as planting in southern China. Our study contributes to a better understanding of the nutrient profiles of D. officinale through artificial cultivation in different areas.

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“…Cytotoxicity screening was accomplished using previously reported methods. , Five cell lines, including C6 rat glioma cells (ATCC #CCL-107), Huh1 (JCRB #0199) and HCC-LM3 (Biobw #bio-106200) human hepatocellular carcinoma cells, PANC-1 human pancreatic cells (ATCC #CRL-1469), and Hela human cervical cancer cells (ATTC #CCL-2), were cultured according to each manufacturer’s suggested protocols. Cells were cultured in T75 flasks (part no.…”

Section: Experimental Sectionmentioning

confidence: 99%

Cytotoxic Guaianolide Sesquiterpenoids from Ainsliaea fragrans

et al. 2021

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Twelve guaianolide-type sesquiterpene oligomers with diverse structures were isolated from the whole plants of Ainsliaea fragrans, including a novel trimer (1) and two new dimers (2, 3). The chemical structures of the new compounds were elucidated through spectroscopic data interpretation and computational calculations. Ainsfragolide ( 1) is an unusual guaianolide sesquiterpene trimer generated with a novel C−C linkage at C 2' −C 15″ , which may be biosynthesized prospectively through a further Michael addition. Cytotoxicity results showed that ainsfragolide (1) was the most potent compound against five cancer cell lines with IC 50 values in the range of 0.4−8.3 μM.

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New peptidendrocins and anticancer chartreusin from an endophytic bacterium of Dendrobium officinale

Cited by 18 publications

References 28 publications

Taxonomic and functional diversity of Dendrobium officinale microbiome in Danxia habitat

Taxonomic and functional diversity of Dendrobium officinale microbiome in Danxia habitat

Comparative Analysis of Nutritional Quality of Dendrobium Officinale Kimura Et Migo After Transplantation of Artificially Cultivated Plants From South to North China During Different Growing Years

Cytotoxic Guaianolide Sesquiterpenoids from Ainsliaea fragrans

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