Taxonomic and functional diversity of Dendrobium officinale microbiome in Danxia habitat

Abstract

Aims Microbial communities that inhabit plants are crucial for plant survival and well‐being including growth in stressful environments. The medicinal plant, Dendrobium officinale grows in the barren soils of the Danxia Habitat. However, the microbiome composition and functional potential for growth of this plant in this environment are still unexplored. Methods and Results In this study, we analysed the taxonomic and functional diversity of the D. officinale Microbiome by metagenomic sequencing of both rhizosphere and endosphere samples. A total of 155 phyla, 122 classes, 271 orders, 620 families and 2194 genera were identified from all samples. The rhizospheric microbes (DXRh) were mainly composed of Proteobacteria and Acidobacteria, while Basidiomycota and Ascomycota were the most dominant phyla in root endosphere (DXRo) and stem endosphere (DXS), respectively. Most of the dominant microbial communities had been reported to have diverse functional potentials that can help plant growth and development in stressful and nutrient‐deprived ecological environmental. These include plant growth promoting rhizobacteria (PGPR) such as Massilia, Pseudomonas, Bradyrhizobium, Klebsiella, Streptomyces, Leclercia, Paenibacillus, Frankia and Enterobacter in the DXRh, Tulasnella and Serendipita in the DXRo, Colletotrichum and Burkholderia in the DXS and Paraburkholderia, Rhizophagus and Acetobacter in endosphere. Analysis using the KEGG, eggNOG and CAZy databases showed that metabolic pathways such as carbohydrate metabolism, amino acid metabolism, energy metabolism, genetic information processing and environmental information processing are significantly abundant, which may be related to the survival, growth and development of D. officinale in a stressful environment. Conclusions We speculated that the microbial community with diverse taxonomic structures and metabolic functions inhabiting in different niches of plants supports the survival and growth of D. officinale in the stressful environment of Danxia Habitat. Significance and Impact of the Study This study provided an important data resource for microbes associated with D. officinale and theoretical foundation for further studies.

Probiotic fermentation improves the bioactivities and bioaccessibility of polyphenols in Dendrobium officinale under in vitro simulated gastrointestinal digestion and fecal fermentation

“…In addition, phenolic acids and their derivatives including syringic acid, dihydro ferulic acid tyramine, p-hydroxybenzoic acid, ellagic acid, and gallotannins were also discovered in DO. These PPs were shown to have various biological activities, such as antioxidant, hypoglycemic, anticancer, antimicrobial, and immunomodulatory activities ( 7 , 8 ).…”

Comparative analysis of endophyte diversity of <i>Dendrobium officinale</i> lived on rock and tree

“…It has been found that endophytic fungi of D. officinale mainly include 57 genera of Basidiomycota, Ascomycota and Deuteromycotina, and endophytic bacteria mainly include 50 genera of Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes and Fusobacteria ( Santoyo et al 2016 ; Sarsaiya et al 2020 ; Wang et al 2022 ; Zhang et al 2019 ). In this study, the endophytic fungi in the roots of D. officinale were mainly Basidiomycota and Ascomycota, and the endophytic bacteria were mainly Proteobacteria, Actinomycetes, Firmicutes, Bacteroides, Acidobacteria, etc.…”