New Pentastomida. Sambonia parapodum n. sp. from Varanus salvator, and Armillifer agkistrodontis n. sp. from Agkistrodon acutus

Complete Mitochondrial Genome of a Tongue Worm Armillifer agkistrodontis

“…These pentastomids were washed with saline buffer (0.7% NaCl) before being fixed in 70% (v/v) ethanol at 4°C. Species and sexual identification of pentastomids was defined by morphological characteristics [ 7 ], after which the tegument was isolated from individual females by excision under a stereomicroscope. Total genomic DNA was extracted from this tegument tissue using a DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions, and stored at −20°C until use.…”

Some observations on the taxonomy and systematics of the pentastomid genus Armillifer (Sambon, 1922) in south east Asian and Australian snakes

“…Three species, A. armillatus, A. grandis and A. agkistrodontis are well characterized (see Fain, 1961;Self & Kuntz, 1966) but as mentioned earlier, the taxonomic status of A. moniliformis is altogether less certain. The confusion as to the exact number of species has its foundation in the lack of good diagnostic characters, and/or type descriptions based on few specimens.…”

“…The type description (Mazza, 1891) is based on five females collected from an unknown host in New Guinea. Incomplete annuli are typical of all the described species of Armillifer (see Fain, 1961) with the exception of A. agkistrodontis (see Self & Kuntz, 1966). Specimens subsequently examined by Heymons (1932), also from an unknown host, were in poor condition and the posterior portion of the abdomen was flattened, thereby precluding useful observation.…”