New Oral Dosage Form for Elderly Patients. II. Release Behavior of Benfotiamine from Silk Fibroin Gel.

Hanawa, Takehisa; Watanabe, Atsushi; Tsuchiya, Teizo; Ikoma, Rieko; Hidaka, Masato; Sugihara, Masayasu

doi:10.1248/cpb.43.872

Cited by 47 publications

(31 citation statements)

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“…It is also available for similar indications as a homeopathic medicine and as a dietary supplement. The typical oral dose recommended is 40-450 mg/day [63,64]. The topical application of benfotiamine may allow more prodrug to be introduced into the target tissues and, as a result, potentially generate a greater therapeutic effect.…”

Section: Introductionmentioning

confidence: 99%

Efficient Transdermal Delivery of Benfotiamine in an Animal Model

2015

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We designed a transdermal system to serve as a delivery platform for benfotiamine utilizing the attributes of passive penetration enhancing molecules to penetrate through the outer layers of skin combined with the advance of incorporating various peripherally-acting vasodilators to enhance drug uptake. Benfotiamine, incorporated into this transdermal formulation, was applied to skin in an animal model in order to determine the ability to deliver this thiamine pro-drug effectively to the sub-epithelial layers. In this proof of concept study in guinea pigs, we found that a single topical application of either a solubilized form of benfotiamine (15 mg) or a microcrystalline suspension form (25 mg) resulted in considerable increases of the dephosphorylated benfotiamine (S-benzoylthiamine) in the skin tissue as well as in significant increases in the thiamine and thiamine phosphate pools compared to control animals. The presence of a ~8000x increase in thiamine and increases in its phosphorylated derivatives in the epidermis

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Section: Introductionmentioning

confidence: 99%

Efficient Transdermal Delivery of Benfotiamine in an Animal Model

2015

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“…Silk fibroin (SF) consists of heavy chain (~390 kDa) and light chain (~25 kDa) polypeptides linked by a disulfide bond (Zhou et al, 2001). Many researchers consider that silk is one of the promising materials for application in delivery of hydrophobic drugs and enzymes (Hanawa et al, 1995;Oh and Lee, 2013;Kuzuhara et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Silk Beads for Protein Delivery System

Kim¹,

Jo²,

Lee³

et al. 2014

International Journal of Industrial Entomology

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In order to the feasibility of silk materials as protein delivery system, silk beads incorporated with bovine serum albumin (BSA) were prepared by dropping silk fibroin extract into dope solution composed of ethanol and dichloromethane . Structural and morphological characteristics of silk beads were examined using scanning electron microscopy (SEM), infrared spectrometry, and X-ray diffractometry. Swelling ratio of silk beads was also measured. Release behavior of prototypical protein, BSA, was studied by observing the electropheretic phenomenon and release profile. SEM showed that silk beads are spherical with porous interior structure. Infrared spectrometry and X-ray diffraction confirm that the silk beads have a b-sheet conformation. The swelling capability of silk beads increased with the incorporation of the protein. The protein was released from the beads with slow release following an initial burst release. Therefore, silk beads show promise as materials for encasing protein drugs to be delivered to targets in the human body.

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“…Traditionally, silkworm cocoon has been used to make a textile fiber and surgical suture for several thousand years. Recently useful biological properties of silk protein have been reported by researchers; acceleration of collagen formation, and proliferation of cultured human skin fibroblasts (Yeo et al, 2000;Yamada et al, 2004), matrix for mammalian cell culture and enzyme immobilization (Minoura et al, 1995), scaffold for bone substitution (Sofia et al, 2001), and drug delivery carrier (Hanawa et al, 1995), oxygen and water vapor permeability Minoura et al, 1990;Kweon et al, 2001, and blood compatibility (Sakabe et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Tetracycline-incorporated Silk Fibroin Films

Kim¹,

Shin²,

Jeon³

et al. 2012

International Journal of Industrial Entomology

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Silk fibroin films incorporated with tetracycline was prepared and characterized by Fourier-transform infrared spectrometer and differential scanning calorimeter and examined antibacterial effect. The conformation of silk fibroin was changed from random coil to â sheet structure with incorporation of tetracycline. Antibacterial activity of the materials was evaluated against Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Staphylococcus epidermis, and Staphylococcus aures) using agar plate method showing clear inhibition zone around tetracycline silk film. It is concluded that tetracycline-incorporated SF films are highly effective against bacteria.

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New Oral Dosage Form for Elderly Patients. II. Release Behavior of Benfotiamine from Silk Fibroin Gel.

Cited by 47 publications

References 1 publication

Efficient Transdermal Delivery of Benfotiamine in an Animal Model

Efficient Transdermal Delivery of Benfotiamine in an Animal Model

Preparation and Characterization of Silk Beads for Protein Delivery System

Tetracycline-incorporated Silk Fibroin Films

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