New nanocomposite films for biosensors: layer-by-layer adsorbed films of polyelectrolytes, proteins or DNA

Decher, Gero; Lehr, B.; Lowack, K.; Lvov, Yuri; Schmitt, Johannes

doi:10.1016/0956-5663(94)80065-0

Cited by 385 publications

(273 citation statements)

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“…One advantage of ESA is that the chemical properties of the polymers are preserved after the multilayer buildup. Thus some interesting material can be bonded on the films by chemical interactions such as hydrogen bonds [67], covalent bonds [68], and specific recognition [69]. Much work has been generalized to incorporate molecules, proteins and macromolecules into polymers [62,64,[70][71][72][73].…”

Section: Layer-by-layer Electrostatic Self-assembly Methodsmentioning

confidence: 99%

Microgap Structured Optical Sensor for Fast Label-Free DNA Detection

Wang

Cooper

Wang

2008

J. Lightwave Technol.

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DNA detection technology has developed rapidly due to its extensive application in clinical diagnostics, bioengineering, environmental monitoring, and food science areas.Currently developed methods such as surface Plasmon resonance (SPR) methods, fluorescent dye labeled methods and electrochemical methods, usually have the problems of bulky size, high equipment cost and time-consuming algorithms, so limiting their application for in vivo detection. In this work, an intrinsic Fabry-Perot interferometric (IFPI) based DNA sensor is presented with the intrinsic advantages of small size, low cost and corrosion-tolerance. This sensor has experimentally demonstrated its high sensitivity and selectivity.In theory, DNA detection is realized by interrogating the sensor's optical cavity length variation resulting from hybridization event. First, a microgap structure based IFPI sensor is fabricated with simple etching and splicing technology. Subsequently, considering the sugar phosphate backbone of DNA, layer-by-layer electrostatic self-assembly technique is adopted to attach the single strand capture DNA to the sensor endface. When the target DNA strand binds to the single-stranded DNA successfully, the optical cavity length of sensor will be increased. Finally, by demodulating the sensor spectrum, DNA hybridization event can be judged qualitatively.This sensor can realize DNA detection without attached label, which save the experiment expense and time. Also the hybridization detection is finished within a few minutes.This quick response feature makes it more attractive in diagnose application. Since the iii sensitivity and specificity are the most widely used statistics to describe a diagnostic test, so these characteristics are used to evaluate this biosensor. Experimental results demonstrate that this sensor has a sensitivity of 6nmol/ml and can identify a 2 bp mismatch. Since this sensor is optical fiber based, it has robust structure and small size ( 125μm ). If extra etching process is applied to the sensor, the size can be further reduced. This promises the sensor potential application of in-cell detection. Further investigation can be focused on the nanofabrication of this DNA sensor, and this is very meaningful topic not only for diagnostic test but also in many other applications such as food industry, environment monitoring.iv Acknowledgement

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Section: Layer-by-layer Electrostatic Self-assembly Methodsmentioning

confidence: 99%

Microgap Structured Optical Sensor for Fast Label-Free DNA Detection

Wang

Cooper

Wang

2008

J. Lightwave Technol.

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“…Esses grupos iônicos em solução aquosa são parcialmente ionizados, e assim diferentes densidades de carga no polímero são obtidas. Baseada nessa propriedade, a formação de complexos polieletrolíticos pela interação entre poliânions e policátions na interface levou ao desenvolvimento de materiais híbridos nanoestruturados na forma de filmes finos (DECHER et al, 1994;DECHER;. Esses polímeros, na sua maioria, são solúveis em solventes polares (SELVAN et al, 1998), e os filmes assim produzidos são denominados filmes LbL.…”

Section: Nanofilmes: Técnica Layer-by-layerunclassified

Nanobiotecnologia: plataforma tecnológica para biomateriais e aplicação biológica de nanoestruturas

Faria-Tischer

Tischer

2012

Biochem. Biotechnol. Rep.

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Nanobiotecnologia: plataforma tecnológica para biomateriais e aplicação biológica de nanoestruturasAbreviaturas: Nanobiotechnology, biomaterials and biological application of nanostructures Nanobiotecnologia, biomateriais e aplicação biológica de nanoestruturas ResumoA nanotecnologia é um campo da ciência que tem se desenvolvido de maneira impressionante, juntamente com a biotecnologia, misturando o conhecimento da ciência dos materiais e da química com os da biologia. Este artigo de revisão busca unir o conhecimento em biomateriais, como carboidratos e proteínas, com aqueles sobre nanopartículas, sistemas auto-organizados, nanofilmes, nanoemulsões e nanoestruturas, para encapsulação de compostos ativos. O impacto econômico dos produtos de nanotecnologia está na ordem dos trilhões de dólares. A nanobiotecnologia divide uma grande parte desse mercado. Os setores que alcançam benefícios com o desenvolvimento de produtos, técnicas, metodologias e processos em escala nanométrica são: saúde, alimentos e agroindústria. Este representa um campo fértil para aplicações em grande volume. A nanobiotecnologia é uma realidade e o conhecimento de biomateriais, das ciências biológicas e da engenharia, através da união de diferentes grupos de pesquisa em seus campos específicos, permite a concepção de produtos nunca imaginados há alguns anos. Palavras AbstRActNanotechnology is a science field that has been developping amazingly together with the biotechnology, mixing the knowledge of material science and chemistry with the biology. This review article links the know-how of biomaterials in the recent years, like carbohydrates and proteins with those about nananoparticles, self-assembled systems, nanofilms, nanoemulsions and nanoestructures for active compounds for encapsulation. The economical impact of nanotech products is in order of trillions of US dollars, and nanobiotechnology has a great share of this market. The sectors that have benefits with the development of products, technics, methodology and processes on nanometric scale are the health, food and agroindustry. The last one represents a fertile field for applications on a large scale. The nanobiotechnology is a reality and the knowledge of biomateriais, biological sciences and engineering, through the union of different research groups on their specific fields, allows the conception of products never considered many years ago.

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“…As one of the most suitable and versatile approach to fabricate functional two-dimensional or three-dimensional thin aqueous films, electrostatic layer-by-layer self-assembly based on the work of Decher and co-workers has found many applications in molecular and nanoscience technology [4][5][6][7]. The key advantage of this approach is the possibility of assembling a number of different hybrid materials such as polyelectrolytes, biological components, nanoparticles and photoactive dyes [4][5][6][7][8][9][10] thanks to the alternate deposition of layers of opposite surface charges.…”

Section: Introductionmentioning

confidence: 99%

“…The key advantage of this approach is the possibility of assembling a number of different hybrid materials such as polyelectrolytes, biological components, nanoparticles and photoactive dyes [4][5][6][7][8][9][10] thanks to the alternate deposition of layers of opposite surface charges. Other important features include the simple deposition process and the possibility to control their organization at the molecular level as well as to control the thickness easily by varying the number of adsorbed layers.…”

Section: Introductionmentioning

confidence: 99%