New nano-hydroxyapatite in bone defect regeneration: A histological study in rats

Kubasiewicz−Ross, Paweł; Hadzik, Jakub; Seeliger, Julia; Kozak, Karol; Jurczyszyn, Kamil; Gerber, Hanna; Dominiak, Marzena; Kunert‐Keil, Christiane

doi:10.1016/j.aanat.2017.05.010

Cited by 59 publications

(37 citation statements)

References 40 publications

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“…To examine the new biomaterial HA scaffolds (control) and Cu 5 -HA (i.e., the best result from the in vitro test), suitable skeleton skin defects in a poorly vascularized location ( Figure 2 c) must be established in vivo through the use of appropriate animal models. The in vivo animal model allows for the standardization or elimination of variables that contribute to the success or failure of tissue engineered materials [ 22 ]. Figure 9 a,b demonstrate that no blood vessels formed on the HA and Cu 5 -HA scaffolds after one week of implantation.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Micro/Nano-Structure Copper-Substituted Hydroxyapatite Scaffolds with Improved Angiogenesis Capacity for Bone Regeneration

et al. 2018

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The surface microstructures of calcium phosphate ceramics play an essential role in determining bone regeneration. However, it is difficult to produce micro/nano-structures on the surface of the porous hydroxyapatite (HA) scaffolds. In this study, we successfully developed and fabricated various micro/nano-structured surfaces on the HA scaffolds in copper ion (Cu2+)-containing solutions under hydrothermal conditions. The micro/nano-structures on the surface of the HA scaffolds were controlled by modulating the Cu2+ concentrations during the hydrothermal process. With an increase in the Cu2+ concentration, the surface morphology of the HA scaffolds changed significantly from sphere-like to flower-like, before becoming nano-structures. These findings indicated that the Cu2+ concentration affects the morphologies of calcium phosphate coatings that grow on the HA scaffolds. In vitro endothelial cell (EC) cultures showed that the cell proliferation was significantly enhanced when cultured on the flower-like morphology compared with other morphologies. Furthermore, an in vivo test in New Zealand rabbits demonstrated that the HA scaffold with the flower-like surface resulted in more angiogenesis compared with the control scaffold. This copper-assisted hydrothermal deposition process provides a simple and controllable route for engineering a micro/nano-structured surface on the HA scaffolds, which has benefits in terms of angiogenesis and bone regeneration.

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Section: Resultsmentioning

confidence: 99%

Preparation of Micro/Nano-Structure Copper-Substituted Hydroxyapatite Scaffolds with Improved Angiogenesis Capacity for Bone Regeneration

et al. 2018

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“…These data indicate that the scaffold has good biocompatibility: human MSCs adhered to and proliferated on the surface of the scaffold, and their metabolic activity was normal. The similarity of the XRD TEM, and BET results of natural bone and collagen fibers mineralized with nanosized HA, [6][7][8][9][10][11][12][13][14][15][16][17][18] may explain the biomimetic effect in terms of osteointegration, cell seeding, and formation of new bone. However the hydrophobic PCL could hamper differentiation of MSCs.…”

Section: Biocompatibility: Human Mscs and Hepg2/saos-2 Cells Complemementioning

confidence: 99%

“…Nano-hydroxyapatite (nHA), which is composed of the same ions as the major mineral constituent of bone, is an attractive implant material for bone regeneration due to its biocompatibility and osteoconductivity properties. Furthermore, it does not cause systemic toxicity or immunological reactions [13][14][15]. Porous HA scaffolds are used in bone-tissue engineering, as their architecture mimics very closely that of the bone extracellular matrix (bECM), thereby providing a favorable environment for biological processes [16][17].…”

Section: Introductionmentioning

confidence: 99%

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Nanostructured surface bioactive composite scaffold for filling of bone defects

2020

Biointerface Res Appl Chem

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The aim of this study was to investigate the chemical and physical surface properties of a hybrid nano-hydroxyapatite/collagen/polycaprolactone (nHA/Coll/PCL) material, and to test its in vitro biocompatibility and in vivo osteointegration. Mineralized collagen fibers with nHA were admixed with PCL at a weight proportion of 50:50. The material was characterized by transmission X-ray diffraction (XRD), electron microscopy (TEM), atomic force microscopy (AFM), force spectroscopy, X-ray Photoemission Spectroscopy (XPS), and biocompatibility testing using human mesenchymal stem cells (MSCs), and Hepatocyte carcinoma (HePG2) and primary osteogenic sarcoma (SAOS-2) cells as complementary tests. In addition, the ability of this material to fill three-wall bony defects was tested in the mandible of a sheep. The material had confirmed the relative low crystallinity of the HA having a nano-sized dimension, which was composed of only oxygen, carbon, calcium and phosphorus, without no residual cytotoxic element. Human MSCs on the surface scaffold showed high metabolic activity and a high rate of viability. Biocompatibility complementary testing using HePG2 and SAOS-2 cells showed good metabolic activity, and the lactate dehydrogenase assay using HePG2 cells demonstrated no significant cytotoxicity. Histological analysis of the in-vivo experimentation showed osteointegration of the material and the absence of inflammatory cells at the bone–scaffold interface. Some areas showed bone-cell seeding and isolated agglomerates of bone cells were evident in the inner scaffold.

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“…11,12 However, HA do not show any intrinsic bioactivity, and do not induce positive regenerative response from bone cells when implanted. HA has also been shown to have positive effects on bone formation and cell fate in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 99%

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

Guo

Dong

Xiao

et al. 2018

J Biomedical Materials Res

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Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology. However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined. In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO ) and chitosan-silica-hydroxyapatite (CS/SiO /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately. Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids. After intramuscular implantation, CS/SiO and CS/SiO /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration. Naturally dried CS/SiO hybrid provoked a more severe inflammation than the freeze-dried ones. Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants. Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation. Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease. Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO and CS/SiO /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1223-1235, 2018.

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New nano-hydroxyapatite in bone defect regeneration: A histological study in rats

Cited by 59 publications

References 40 publications

Preparation of Micro/Nano-Structure Copper-Substituted Hydroxyapatite Scaffolds with Improved Angiogenesis Capacity for Bone Regeneration

Preparation of Micro/Nano-Structure Copper-Substituted Hydroxyapatite Scaffolds with Improved Angiogenesis Capacity for Bone Regeneration

Nanostructured surface bioactive composite scaffold for filling of bone defects

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

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New nano-hydroxyapatite in bone defect regeneration: A histological study in rats

Cited by 59 publications

References 40 publications

Preparation of Micro/Nano-Structure Copper-Substituted Hydroxyapatite Scaffolds with Improved Angiogenesis Capacity for Bone Regeneration

Preparation of Micro/Nano-Structure Copper-Substituted Hydroxyapatite Scaffolds with Improved Angiogenesis Capacity for Bone Regeneration

Nanostructured surface bioactive composite scaffold for filling of bone defects

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO2 and chitosan/SiO2/hydroxyapatite hybrids

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In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids