New molecular diagnosis method combined with nucleic acid sequence-based amplification and probe amplification in rapid detection of Enterovirus 71

Abstract

In this study, a new molecular diagnosis assay for rapid detection of virus RNA was established, which combined nucleic acid sequence-based amplification (NASBA) with rapid isothermal detection assay (RIDA). Using this method, we could rapidly identify Human enterovirus 71 RNA with high sensitivity and specificity; the detection limit of the new method was 10 2 copy/ml. The whole assay was processed in one tube and the result was determined by naked eyes under ultraviolet radiation without capopening, meanwhile, as the products of amplification was RNA which is easily degradative, thus, the aerosol contamination was further decreased. This novel method shows excellent sensitivity, specificity and conveniences, which is easily operated in the elementary medical organizations and resource-limited areas.

Potential relevance of salivary legumain for the clinical diagnostic of hand, foot, and mouth disease

“…Therefore, the development of HFMD point‐of‐care testing (POCT) is imperative to assist clinical diagnosis as well as timely clinical isolation and treatment regime for the actual cases. As HFMD can be caused by a myriad of human enteroviruses, a readily available on‐site diagnostic test had been conceptualized, 20–26 based on molecular and serological diagnostic methods. However, previous attempts tapping on virus dynamics are a crucial bottleneck to providing a complete diagnostic coverage of the nonexhaustive spectrum of HFMD‐causing viruses.…”

“…Therefore, the development of HFMD point-of-care testing (POCT) is imperative to assist clinical diagnosis as well as timely clinical isolation and treatment regime for the actual cases. As HFMD can be caused by a myriad of human enteroviruses, a readily available on-site diagnostic test had been conceptualized, [20][21][22][23][24][25][26] based on molecular and serological diagnostic methods.…”