New methods for haploid selection in maize

Tseng, Yichia

doi:10.31274/etd-180810-256

Cited by 3 publications

(4 citation statements)

References 42 publications

(36 reference statements)

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“…The CDR changed for each donor, ranging from 22.5 to 48.3%. Doubling rates have reached 50%, 0-40%, and 55.31%, as reported by Vanous (2011), Tseng (2012) and Prasanna et al (2012), respectively. Cengiz (2016) reported that fertile plants made up 57% of live plants.…”

Section: Chromosome Doubling Rate (Cdr)supporting

confidence: 72%

“…The in vivo production of maternal DHL involves the following four steps: (i) inducing haploidy by pollinating the source germplasm with pollen of the haploid inducer; (ii) identifying those seeds with haploid embryos based on a visual scorable morphological marker; (iii) duplicating chromosomes of putative haploids by treating the seedlings with a mitotic inhibitor; and (iv) selfpollinating DH plants to multiply their seed (Prigge 2012;Prasanna et al 2012;Tseng 2012). To induce maternal haploids, the donor plant is pollinated by a specific maize stock (line, single cross or population), which is called the inducer (Geiger & Gordillo 2009).…”

Section: Introductionmentioning

confidence: 99%

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Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Zararsiz¹,

Öztürk²,

Yanikoğlu³

et al. 2019

Tarım Bilimleri Dergisi

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This research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursa in Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donor materials were obtained during the 2012 winter season in the greenhouse. The inducer line RWK-76xRWS, provided by University of Hohenheim, Germany, was used for generating haploid seeds. The donor and inducer crossing was performed during the 2013 summer season. The haploid selection and chromosome doubling were performed during the 2014 summer season. Seven donors were used for haploid induction which name are DNR1, DNR2, DNR3, DNR4, DNR5, DNR6, DNR7 respectively, from each donor different amount of ear crosses were performed (DNR1:16 ears, DNR2:10 ears, DNR3:10 ears, DNR4:12 ears, DNR5:11 ears, DNR6:13 ears, DNR7:11 ears). According to the present study, the average induction rate found ranged from 7.1 to 12.8%, and the average seedling survival rate in the greenhouse after colchicine application ranged from 57.9 to 77.6%. After transplanting to the field, 78.3-92.6% of these plants survived. As a result of this research, the chromosome doubling rate ranged from 22.5 to 48.3% depending on the donor material. These result indicates that maternal haploid selection visually is easy. Haploid induction rate (HIR) changes from donor to donor, its mean genotype and environment is effective for HIR. Average chromosome doubling rate is lower than other researchers' results, it is also effected by genotype and chromosome doubling methods.

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Section: Chromosome Doubling Rate (Cdr)supporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Zararsiz¹,

Öztürk²,

Yanikoğlu³

et al. 2019

Tarım Bilimleri Dergisi

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“…parent could be used for haploid identification assuming that a single dominant gene is responsible for sensitivity, whereas the recessive form is not sensitive to herbicides (Tseng, 2012). After pollination of selected parents, either heterozygous F 1 or haploid progeny is produced.…”

Section: Crop Sciencementioning

confidence: 99%

“…Green fluorescence could be used for machine sorting to detect haploids; if a haploid inducer has the green fluorescent protein (GFP) gene, then the hybrid progeny will produce fluorescence, whereas haploids will not. As proposed by Tseng (2012), GFP markers could be explored for their potential use in haploid selection.…”

Section: Other Potential Opportunities For Haploid Selectionmentioning

confidence: 99%

Doubled haploids in maize: Development, deployment, and challenges

Maqbool¹,

Beshir²,

Khokhar³

2020

Crop Science

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Haploids are naturally produced in maize (Zea mays L.) at different rates and can also be induced through different methods. Haploids are used to develop doubled haploids (DHs), which have many potential uses. The development of DH lines in maize involves haploid induction, haploid identification, chromosome doubling, and field sowing for self‐pollination of D0 plants. Different potential methods are used for haploid induction, in‐vivo maternal haploid induction being the most prevalent. Haploid induction is highly reliant on the unambiguous identification of haploids among a mixture of different ploidies. Haploid identification is facilitated by visual morphological markers, chromosome counting, flow cytometry, molecular markers, and many other approaches. Chromosome doubling may be achieved by spontaneous doubling or by induction with different antimicrotubular treatments. Among the potential uses of DH lines are the development of inbred lines, genomic selection (GS), quantitative trait loci (QTL) mapping, and unlocking new genetic variations. Although DH technology can potentially accelerate maize breeding, it still faces challenges at each step of DH line development. This article aims to highlight the importance, procedural steps, potential opportunities, and key challenges in DH line development in maize.

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Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize

Kumar

Chandel

Guleria

2021

Israel J. Plant Sci.

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An investigation to optimize the protocol for application of colchicine for enhancing the doubled haploid production in maize was done. 106 maize genotypes were used as maternal parents, whereas, pollen source involved tropically adopted haploid inducer (TAIL P1 and TAIL hybrid). After the elimination of chromosomes of inducer lines, haploid seeds were obtained from the crosses. Haploid seedlings were treated with three different doses, such as 0.04, 0.06 and 0.08 per cent of colchicines for different durations (8, 12 and 15 hours). The response of various colchicine concentrations applied for different time durations revealed significant differences at P ≤ 0.05 for various parameters viz., per cent plants survivability, stalk colour, the fertility of tassel, silk present/absent, pollen viability, seed set and per cent doubled haploid formation. In maize, colchicine doses of 0.04 per cent for 12 hours and 0.06 per cent for 8 hours, respectively were established as optimum for enhanced doubled haploid production. But among these two, 0.04 per cent for 12 hours was observed to be best dose for doubled haploid production in maize.

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New methods for haploid selection in maize

Cited by 3 publications

References 42 publications

Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Doubled haploids in maize: Development, deployment, and challenges

Optimization of chromosome doubling treatment for efficient in vivo doubled haploid production in maize

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