New method of analysis based on room-temperature phosphorescence

Paynter, R. A.; Wellons, Stephen L.; Winefordner, J. D.

doi:10.1021/ac60342a044

Cited by 96 publications

(40 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phosphorescent labels for phenols are under study in this laboratory (E. U. Akusoba and J. N. Miller, unpublished work). The apparatus described can also be used without liquid nitrogen for analyses by the newly-developed technique of room-temperature phosphorimetry (13).…”

Section: Discussionmentioning

confidence: 99%

“…California, Chemistry Department, electrical conductivity ( I O ) , and ionization ( 2 1 ) ; (2) optoacoustic techniques for gas (12) and condensed phases (13,14); (3) detectors for thin-layer chromatography (25) and high pressure liquid chromatography (16,177); (4) detection of trace contaminates in solids (18, 19); (5) detection of trace species in solution by fluorescence techniques (20-26). The many capabilities offered by laser technology invariably give rise to confusion as to which laser is most appropriate for a given application.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Solvent enhancement effects in thin-layer phosphorimetry

Miller¹,

Phillipps²,

Burns³

et al. 1978

Anal. Chem.

View full text Add to dashboard Cite

The construction and use of an improved thin-layer phosphorimeter is described. The device permits flexible chromatography media to be scanned at 77 K, and also allows a complete characterization of the luminescence properties of the chromatographically-separated solutes. It is shown that the phosphorescence intensities of a variety of adsorbed materials are greatly increased by spraying the chromatography medium with a suitable solvent immediately before examination. The magnitude of the effect depends on the stationary phase, the structure of the adsorbed material, and the solvent sprayed. With the aid of the enhancement effect, nanogram quantities of separated solutes can be analyzed. The instrument Is also suitable for examining the luminescence of adsorbed molecules at ambient temperatures.Combinations of chromatographic methods and fluorescence spectrometry a t room temperature have been repeatedly shown t o be powerful analytical techniques which combine the selectivity of chromatographic separations with the sensitivity of fluorimetry. T h e use of fluorimetry to locate and quantitate the components of complex mixtures separated by thin-layer chromatography (TLC) is a particularly convenient approach (e.g., Ref. 1) which permits the simultaneous study of several very small samples, and instruments designed for the quantitative fluorimetric scanning of TLC plates are commercially available. Although recent studies have amply demonstrated the analytical value of phosphorimetry ( 2 ) , a technique normally performed a t 77 K, combinations of TLC and phosphorimetry have so far been little used. Rinefordner and co-workers determined biphenyl in oranges ( 3 ) , 4-nitrophenol in urine ( 4 ) , and alkaloids in tobacco ( 5 ) by separating the samples on TLC plates, eluting the separated fractions with a suitable solvent, and determining them using conventional phosphorimetric sampling techniques. Studies have also been described in which the phosphorescence of samples separated by TLC have been detected visually by dipping the chromatography plates into liquid nitrogen and observing them under UV illumination. Sawicki et al. (6) investigated a series of aromatic atmospheric pollutants using this approach, and more recently de Silva and Strojny (7) have detected nanogram quantities of various drugs. In the authors' laboratory a thin layer phosphorimeter has been developed (8) as a n accessory suitable for commercially-available spectrofluorimeters. This device permits the in situ quantitative determination and spectroscopic characterization, a t 77 K, of solutes separated on flexible TLC media. The present paper describes the construction and evaluation of a n improved thin-layer phosphorimeter. In particular it is demonstrated that the phosphorescence of solutes adsorbed on TLC plates can sometimes be greatly enhanced by spraying the plates with organic solvents before scanning, a procedure which permits the determination of nanogram quantities of such solutes.'Present address,

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Solvent enhancement effects in thin-layer phosphorimetry

Miller¹,

Phillipps²,

Burns³

et al. 1978

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…There are several ways to diminish the background signal of the paper used as a solid support, the best known being chemical pre-treatment, irradiation with ultraviolet light or extraction with water in a Soxhlet apparatus. [11][12][13][14][15] With these treatments of the paper used as support, the background signal can be 6% of its initial value, but the time of analysis increases and the precision of the measurements can decrease.…”

Section: Introductionmentioning

confidence: 99%

Application of partial least squares multivariate calibration for the determination of mixtures of carbaryl and thiabendazole in waters by transmitted solid phase spectrophosphorimetry

Capitán-Vallvey¹,

Deheidel²,

Orbe³

et al. 1999

Analyst

View full text Add to dashboard Cite

“…These requirements still a r e the main disadvantages which make this spectrochemical method less-widely used than fluorimetry. Recently, the observed phenomenon of intense phosphorescence at room temperature (RTP) from ionic organic compounds adsorbed on a variety of supports, such as silica, alumina, paper, and asbestos [36, 371, h a s been proposed as a new analytical technique [38][39][40][41][42]. A large variety of ionic Downloaded by [Stockholm University Library] at 14:55 10 August 2015 compounds of biological and clinical interest [38][39][40] has been investigated by this new method.…”

mentioning

confidence: 99%

“…Recently, the observed phenomenon of intense phosphorescence at room temperature (RTP) from ionic organic compounds adsorbed on a variety of supports, such as silica, alumina, paper, and asbestos [36, 371, h a s been proposed as a new analytical technique [38][39][40][41][42]. A large variety of ionic Downloaded by [Stockholm University Library] at 14:55 10 August 2015 compounds of biological and clinical interest [38][39][40] has been investigated by this new method. The u s e of external heavy atom perturbers has also been investigated for the determination of trace nonionic compounds, such a s the polyaromatic hydrocarbons [41] .…”

mentioning

confidence: 99%