New mechanistic insights into the motile-to-sessile switch in various bacteria with particular emphasis on <i>Bacillus subtilis</i> and <i>Pseudomonas aeruginosa</i>: a review

Sadiq, Faizan Ahmed; Flint, Steve; Li, Yun; Liu, TongJie; Yuan, Lei; Sakandar, Hafiz Arbab; He, Guoqing

doi:10.1080/08927014.2017.1304541

Cited by 22 publications

(6 citation statements)

References 173 publications

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“…Type IV pili are important surface appendages that are central to the surface-sensing mechanism in the early stages of biofilm formation [13]. QS, twocomponent systems and nucleotide second messengers c-di-GMP [15,16] are considered as the main regulators controlling extracellular polymeric substances (EPS) production during biofilm formation [17]. Two-component systems consist of a histidine kinase and a cognate response regulator [18].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

Liu

Wang

et al. 2021

Microorganisms

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Biofilm formation has evolved as an adaptive strategy for bacteria to cope with harsh environmental conditions. Currently, little is known about the molecular mechanisms of biofilm formation in bifidobacteria. A time series transcriptome sequencing analysis of both biofilm and planktonic cells of Bifidobacterium longum FGSZY16M3 was performed to identify candidate genes involved in biofilm formation. Protein–protein interaction network analysis of 1296 differentially expressed genes during biofilm formation yielded 15 clusters of highly interconnected nodes, indicating that genes related to the SOS response (dnaK, groS, guaB, ruvA, recA, radA, recN, recF, pstA, and sufD) associated with the early stage of biofilm formation. Genes involved in extracellular polymeric substances were upregulated (epsH, epsK, efp, frr, pheT, rfbA, rfbJ, rfbP, rpmF, secY and yidC) in the stage of biofilm maturation. To further investigate the genes related to biofilm formation, weighted gene co-expression network analysis (WGCNA) was performed with 2032 transcript genes, leading to the identification of nine WGCNA modules and 133 genes associated with response to stress, regulation of gene expression, quorum sensing, and two-component system. These results indicate that biofilm formation in B. longum is a multifactorial process, involving stress response, structural development, and regulatory processes.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

Liu

Wang

et al. 2021

Microorganisms

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“…Biofilms are a population of microorganisms that have switched from planktonic to sessile state after adhering to a particular surface [1]. Unlike a planktonic form, biofilms are highly organized, in which biofilm microorganisms interact with chemical signals and create a protective structure that enables them to survive in extreme conditions, such as host defense and antimicrobial agents [2]. When the biofilm is formed, it is very difficult to remove these from the surfaces and thus biofilms can lead to life‐threatening infections in patients [3].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

et al. 2020

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The aim of the present work was to investigate the antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles (AgNPs) fabricated from Mespilus germanica extract against multidrug‐resistant (MDR) Klebsiella pneumoniae strains. Fifty strains of K. pneumoniae were isolated from various clinical specimens. Biofilm‐forming strains were identified using Congo red agar and polymerase chain reaction (PCR) techniques. Subsequently, the antibacterial activity of phytosynthesized AgNPs on MDR K. pneumoniae strains was investigated by broth microdilution assay and agar well‐diffusion method. Finally (in the last step), the antibiofilm activity of phytosynthesized AgNPs was determined using microtiter plate assay and real‐time PCR (RT‐PCR) methods for the analysis of type 3 fimbriae (mrkA) and quorum‐sensing system (luxS) gene expression. The results of this study showed that the phytosynthesized AgNPs had a spherical nanostructure with the mean size of 17.60 nm. The AgNPs exhibited dose‐dependent antibacterial activity. The results of the microtiter plate and RT‐PCR methods show that AgNPs inhibited the biofilm formation in MDR K. pneumoniae strains, and the expressions of mrkA and luxS genes were downregulated significantly in MDR strains after treatment with a subminimum inhibitory concentration of AgNPs. In conclusion, AgNPs effectively prevent the formation of biofilms and kill bacteria in established biofilms, which suggests that AgNPs might be a promising candidate for the prevention and treatment of biofilm‐related infections caused by MDR K. pneumoniae strains.

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“…This was further supported by the concurrent downregulation of motility-related genes and the upregulation of genes potentially involved in attachment or EPS biosynthesis (Fig. 3), demonstrating a transition from free-swimming to a long-lasting, sessile lifestyle 157 . Importantly, these multidimensional structures were found to be held by a bacterially produced ECM (Fig.…”

Section: Discussionmentioning

confidence: 62%

Bacteria Contribute Exopolysaccharides to an Algal-Bacterial Joint Extracellular Matrix

Lipsman,

Shlakhter,

Rocha

et al. 2023

Preprint

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Marine ecosystems are influenced by phytoplankton aggregation, which affects processes like marine snow formation and harmful events such as marine mucilage outbreaks. Phytoplankton secrete exopolymers, creating an extracellular matrix (ECM) that promotes particle aggregation. This ECM attracts heterotrophic bacteria, providing a nutrient-rich and protective environment. In terrestrial environments, bacterial colonization near primary producers relies on attachment and the formation of multidimensional structures like biofilms. Bacteria were observed attaching and aggregating within algal-derived exopolymers, but it is unclear if bacteria produce an ECM that contributes to this colonization. This study, usingEmiliania huxleyialgae andPhaeobacter inhibensbacteria in an environmentally relevant model system, reveals a shared algal-bacterial ECM scaffold that promotes algal-bacterial aggregation. Algal exudates play a pivotal role in promoting bacterial colonization, stimulating bacterial exopolysaccharide (EPS) production, and facilitating a joint ECM formation. A bacterial biosynthetic pathway responsible for producing a succinoglycan-like compound contributing to bacterial ECM formation is identified. Genes from this pathway show increased expression in algal-rich environments. These findings highlight the underestimated role of bacteria in aggregate-mediated processes in marine environments, offering insights into algal-bacterial interactions and ECM formation, with implications for understanding and managing disturbances like marine mucilage events.

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New mechanistic insights into the motile-to-sessile switch in various bacteria with particular emphasis on Bacillus subtilis and Pseudomonas aeruginosa: a review

Cited by 22 publications

References 173 publications

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation

Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

Bacteria Contribute Exopolysaccharides to an Algal-Bacterial Joint Extracellular Matrix

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