New Lanostanoids of Ganoderma tsugae

Abstract

Three new compounds, (24R, S)-3alpha-acetoxy-24-hydroxy-5alpha-lanosta-8,25-di en-21-oic acid, named tsugaric acid C (1); 3alpha-acetoxy-5alpha-lanosta-8, 24-diene-21-O-beta-D-xyloside, named tsugarioside B (2); and 3alpha-acetoxy-(Z)-24-methyl-5alpha-lanosta-8,23,25-tr ien-21-oic acid ester beta-D-xyloside, named tsugarioside C (3), and a mixture of two known steroids were isolated from the fruit bodies of Ganoderma tsugae. The structures of 1-3 were determined by spectral and chemical methods. The cytotoxic activity of the lanostanoid constituents of this fungus was evaluated against several different cancer cell lines.

A comparison of in vitro anticancerous activity and mechanism of ethanolic extracts from different Ganoderma genus

“…Ganoderma triterpene is the main component responsible for the anticancerous activity of Ganoderma (Su et al, 2000;Gonzalez et al, 2002). Except for a few from G. tsugae and G. concinna, most reported anticancerous triterpenes are from G. lucidum (Wu et al, 2001;Gao et al, 2002;Luo and Lin, 2002).…”

Mycosphere Essays 20: Therapeutic potential of Ganoderma species: Insights into its use as traditional medicine

“…Compared with FI0-b, FI0-b-H has more marked effects on human proinflammatory cytokine production (Gao et al 2000). Su et al (2000) examined the cytotoxic activity of lanostanoids from G. tsugae and found activity against three cancer cell lines. Kleinwächter et al (2001) found Lucidenic acids and Colossolactones A inhibit HepG2 cancer cell invasion by acting as inhibitor on the phorbol-12-myristate-13-acetate (PMA)-induced matrix metalloproteinase (MMP-9) expression.…”

Chromatographic and electrophoretic methods for Lingzhi pharmacologically active components

“…The first one involved the extraction of total triterpenes with organic solvents and water [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42]. For example, in the isolation of triterpenes that possess anti-HIV activity, Min et al [26,35,39] extracted triterpenes from the spores of G. lucidum with methanol by refluxing.…”

“…The mobile phases that have been used included methanol-chloroform [16,26,27,[30][31][32][35][36][37]39,48,50,51], dichloroformmethanol [30], hexane-acetone [26,33,35,37,39,52], ethyl acetate-petroleum ether [28,29,48], benzene-methanolwater [32], ethyl acetate-chloroform [31], ethyl acetatedichloromethane [34], petroleum ether-ethyl acetate [29], benzene-methanol-water [32] and chloroform-methanolwater [32,52]. Also, other researchers have reported the methylation of extracted acidic triterpenes with ethereal diazomethane and then the obtained derivatives were subjected to chromatography on silica gel column and preparative HPLC [32,43,44].…”

“…Fruiting body of G. lucidum [29,45,49,51] Ganoderiol F Fruiting body of G. lucidum [51] Spores of G. lucidum [26] Ganodermanondiol Spores of G. lucidum [26] Fruiting body of G. lucidum [37] Ganodermanontriol Fruiting body of G. lucidum [29,37,45,51] Spores of G. lucidum [26] Lucidenic acid A Fruiting body of G. lucidum [36,47,48] [34] differentiation of different species of Ganoderma by Su et al [56]. They found that different species of Lingzhi exhibited unique triterpene patterns on the TLC chromatograms.…”