Chromatographic and electrophoretic methods for Lingzhi pharmacologically active components

Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities

“…Commonly known as Lingzhi, Ganoderma comprises the most studied species of medicinal mushrooms in the world. In ancient China, Lingzhi was believed to bring longevity, due to its mysterious power of healing the body and calming the mind (Huie and Di, 2004).…”

Current and future perspective on antimicrobial and anti-parasitic activities of Ganoderma sp.: an update

“…Solvents like MeOH, EtOH, CH 2 Cl 2 , CHCl 3 and aqueous – both cold and hot – are used for further purifications and isolation. Techniques such as thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and column chromatography (CC) are used to facilitate the purification and isolation process (Huie & Di 2004). The general procedures of the isolation of antimicrobial and anti-parasitic compounds are shown in Figure 3.…”

“…Currently bioassay-guided antibiotics identification, TLC and chromatography bio-autography are used to track antibacterial ingredients from the extract (Huie & Di 2004). Minimum inhibitory concentration (MIC) and 50% inhibitory concentration (IC 50 ) values are used to determine the potency of antibacterial agents.…”

Traditional Chinese Herbal Medicine – East Meets West in Validation and Therapeutic Application

“…Recently, Askin and co-workers (Askin et al, 2010) have used a hydrothermal extraction method with 'subcritical water', conditioned at 473 K temperature at 10 MPa atmospheric pressure, and successfully obtained the maximum total amount (57.4% yielding) of -glucans from Lingzhi. Besides, extraction of total triterpenes with organic solvents and water have long been practiced, however, isolation of the acidic triterpenes, mainly highly oxygenated triterpenoids which responsible for the bitter taste of the Lingzhi, from the total triterpenes fraction can be achieved with 95% aqueous ethanol under reflux www.intechopen.com and evaporation under reduced pressure, followed by chloroform extraction under acidic condition (Huie & Di, 2004). Silica gel column chromatography has also been described as an additional purification procedure for such acidic triterpenes (Huie & Di, 2004).…”