New label free CA125 detection based on gold nanostructured screen-printed electrode

Ravalli, Andrea; Santos, Glauco Pilon dos; Ferroni, Matteo; Faglia, Guido; Yamanaka, Hideko; Marrazza, Giovanna

doi:10.1016/j.snb.2012.10.017

Cited by 106 publications

(38 citation statements)

References 36 publications

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“…Ravalli et al [41] reported a label-free impedimetric immunosensor for the detection of cancer antigen 125 (CA 125), a protein associated with epithelial ovarian cancer. A self-assembled monolayer (SAM) was formed on the surface of GNPs electrodeposited on SPEs using a thiolic compound (11-mercaptoundecanoic acid), whose carbossilic groups were coupled with amino groups of anti-CA 125 antibody via 3-(3-dimethylaminopropyl)carbodiimide/N Hydroxysuccinimide (EDCA/NHS).…”

Section: Label-free Innovative Electrochemical Immunosensorsmentioning

confidence: 99%

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Arduini

Micheli

Moscone

et al. 2016

TrAC Trends in Analytical Chemistry

328

166

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A B S T R A C TThis review addresses recent advances in the development of screen-printed electrode based biosensors modified with different nanomaterials such as carbon nanotubes, graphene, metallic nanoparticles as gold, silver and magnetic nanoparticles, and mediator nanoparticles (Prussian Blue, Cobalt Phthalocyanine, etc.), coupled with biological recognition elements such as enzymes, antibodies, DNA and aptamers to obtain probes with improved analytical features. Examples of clinical applications are illustrated, together with examples of paper-based electrochemical devices, of multiple detections using arrays of screen printed electrodes, and of the most recent developments in the field of wearable biosensors. Also the use of smartphones as final detectors is briefly depicted.

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Section: Label-free Innovative Electrochemical Immunosensorsmentioning

confidence: 99%

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Arduini

Micheli

Moscone

et al. 2016

TrAC Trends in Analytical Chemistry

328

166

View full text Add to dashboard Cite

show abstract

“…Electrodeposition of gold nanoparticles (AuNPs) on the surface of graphite screen-printed electrodes (GSPEs) was realized in accordance with the optimized procedure reported in our previous work [37]. Briefly, 50 μL of 0.6 mM of HAuCl 4 solution, prepared in 0.5 M H 2 SO 4 , was placed onto GSPE surface.…”

Section: Electrodeposition Of Gold-nanoparticles Onto Graphite Screenmentioning

confidence: 99%

“…Graphite screen-printed electrodes (GSPEs) were modified by electrodeposition of gold nanoparticles (AuNPs) using cyclic voltammetry in accordance with the optimized procedure reported previously [37]. The graphite screen-printed electrodes and gold nanostructured screen-printed electrodes were analyzed by cyclic voltammetry using 0.01 M [Fe(CN) 6 ] 3−/4− as redox probe at different scan-rates (ranging from 0.025 to 0.4 V/s).…”

Section: Electrochemical Characterization Of Aunp-modified Graphite Smentioning

confidence: 99%

A label-free electrochemical affisensor for cancer marker detection: The case of HER2

Ravalli

Rocha

Yamanaka

et al. 2015

Bioelectrochemistry

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“…18 Furthermore, naturally rough screen printed carbon electrodes (SPCEs) electrodeposited by AuNPs or nanostructures (NSs) have emerged as a platform for the construction of disposable, inexpensive and sensitive impedimetric immunosensors. [19][20][21] In our previous study the AuNS-electrodeposited SPCEs (AuNS/SPCEs) had a roughness of 4.9-times greater than that of polished gold disk electrodes. 21 The LOD of rough AuNS/SPCE-based immunosensors for salbutamol (SAL) detection was as low as 4 fg mL -1 .…”

Section: Introductionmentioning

confidence: 99%

Effect of the Chain Length of a Modified Layer and Surface Roughness of an Electrode on Impedimetric Immunosensors

Lin

2017

ANAL. SCI.

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An ultrasensitive label-free impedimetric immunosensor is constructed by modifying a 3-mercaptoproponic acid (MPA) monolayer on highly rough gold nanostructure (AuNS)-electrodeposited screen printed carbon electrodes (SPCEs) for the detection of small molecular weight drugs (SMWDs), such as salbutamol (SAL). The SPCEs preoxidized in a 0.1 M H2SO4 solution (called po-SPCEH2SO4) are electrodeposited with the AuNS to increase the roughness factor to 23.64 ± 1.76, larger than the AuNS/po-SPCENaOH or the AuNS/po-SPCEPBS. Furthermore, the MPA modified layer as a link for the anti-SAL immobilization to give the immunosensors an impedimetric signal-to-noise ratio larger than the 11-mercaptoundecanoic acid-and 16-mercaptohexadecanoic acid-modified layer, due to the lower interfacial impedance of the MPA monolayer. The MPA/AuNS/po-SPCEH2SO4-based immunosensors have a wide linear range of 1 fg mL -1 to 1 ng mL -1 and a limit of detection of 0.6 fg mL -1 . Moreover, the immunosensors can practically quantify the SAL concentrations in 1000 times-diluted serum samples with good recovery.

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New label free CA125 detection based on gold nanostructured screen-printed electrode

Cited by 106 publications

References 36 publications

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

A label-free electrochemical affisensor for cancer marker detection: The case of HER2

Effect of the Chain Length of a Modified Layer and Surface Roughness of an Electrode on Impedimetric Immunosensors

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