New Insights into Xanthophylls and Lipidomic Profile Changes Induced by Glucose Supplementation in the Marine Diatom Nitzschia laevis

Abstract

Nitzschia laevis is a candidate microorganism for bioactive compounds (fucoxanthin and eicosapentaenoic acid (EPA)) production. In this study, the impacts of glucose-induced trophic transition on biomass, photosynthesis, pigments, and lipid profiles were examined. The specific growth rate was increased under glucose addition, achieved at 0.47 day−1 (0.26 ± 0.01 day−1 for the group without glucose in medium). However, the photosynthetic parameters and pigments including chlorophylls, fucoxanthin, and diatoxanthin were reduced. The net yield of EPA doubled under glucose addition, reaching 20.36 ± 1.22 mg/L in 4 days. In addition, the alteration in detailed lipid molecular species was demonstrated with a focus on EPA-enriched lipids. The effects of 2-deoxyglucose (2DG) indicated that glucose phosphorylation was involved in glucose-induced regulation. These findings provide novel data for guiding the application of this diatom strain in the functional food industries.

Optimization of Heterotrophic Culture Conditions for the Microalgae Euglena gracilis to Produce Proteins

“…Twenty micrograms of lyophilized samples were methyl esterized with 1% (v/v) sulfuric acid in methanol and methylbenzene with heptadecanoic acid (C17:0, Sigma-Aldrich, St. Louis, MO, USA) as the internal standard. Samples were incubated in a 50 • C water bath overnight and analyzed using a gas chromatography-mass spectrometry (GC-MS) equipped with a DB-WAX column (30 m × 0.25 mm × 0.25 µm) (Agilent, Santa Clara, CA, USA) [47].…”

Recent Development of Advanced Biotechnology in the Oleaginous Fungi for Arachidonic Acid Production

“…In addition to the genetic makeup of each production strain, the fatty acid chain length and unsaturation are also affected by environmental factors such as temperature 51 and glucose concentration. 52 Generally, mild conditions such as low glucose concentration and temperature contribute to the increase of unsaturation. In this review, we summarize recent progress in fermentation regulation for increased ARA synthesis in Figure 2V−VIII.…”