New insights into sperm with total globozoospermia: Increased fatty acid oxidation and centrin1 alteration

Moretti, Elena; Collodel, Giulia; Salvatici, Maria Cristina; Belmonte, Giuseppe; Signorini, Cinzia

doi:10.1080/19396368.2019.1626934

Cited by 25 publications

(27 citation statements)

References 57 publications

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“…Further, by using scanning electron microscopy ( SEM ), they showed that globozoospermic spermatozoa had a larger neck, and the head is surrounded by thin cytoplasm. Recently, a case study conducting electron microscopy analysis also confirmed the morphological features of globozoospermic cells including a round head without acrosome and with the tail coiled around the nucleus as well as immature chromatin (Moretti, Collodel, Salvatici, Belmonte, & Signorini, ). Moreover, some changes in the protein expression and the localisation of proteins such as centrin1 in the sperm centriole were detected around the head of globozoospermic spermatozoa in comparison with the controls (Moretti et al, ).…”

Section: Sperm Parametersmentioning

confidence: 78%

“…Recently, a case study conducting electron microscopy analysis also confirmed the morphological features of globozoospermic cells including a round head without acrosome and with the tail coiled around the nucleus as well as immature chromatin (Moretti, Collodel, Salvatici, Belmonte, & Signorini, ). Moreover, some changes in the protein expression and the localisation of proteins such as centrin1 in the sperm centriole were detected around the head of globozoospermic spermatozoa in comparison with the controls (Moretti et al, ). In addition, in some spermatozoa, twin tails were wrapped around the sperm head (Nardo et al, ).…”

Section: Sperm Parametersmentioning

confidence: 78%

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Globozoospermia syndrome: An update

2019

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Among the factors involved in male infertility, there is a rare morphology disorder called "globozoospermia" that is classified into total globozoospermia and partial globozoospermia (type I and type II, respectively). This syndrome is primarily characterised by the presence of round-headed spermatozoa with cytoskeleton defects around the nucleus and no acrosome. Current data support the negative correlation between globozoospermia and conventional intracytoplasmic sperm injection (ICSI) outcomes, revealing the need for the management of patients undergoing assisted reproduction technology (ART) through more effective treatment techniques. This review highlights the most important characteristics of globozoospermia such as sperm parameters, DNA/chromatin integrity and sperm DNA fragmentation (SDF), as well as genetic features based on the latest knowledge. Additionally, we looked into current progress on fertilisation potential and possible treatment strategies for patients presenting with globozoospermia. K E Y W O R D SDNA fragmentation, globozoospermia, human, intracytoplasmic sperm injection, morphology, spermatozoa

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Section: Sperm Parametersmentioning

confidence: 78%

Section: Sperm Parametersmentioning

confidence: 78%

Globozoospermia syndrome: An update

2019

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“…Interestingly, a reduction in spermatozoa cPLA2 amount, together with an increase of F2-IsoP levels in spermatozoa and seminal plasma, was reported in spermatozoa of a globozoospermic patient (Moretti et al, 2019). However, it has to be considered that globozoospermia is a peculiar genetic condition.…”

Section: Discussionmentioning

confidence: 99%

“…For each sperm pellet suspension, an extraction with ethyl acetate (7 ml) was carried out and the obtained total lipids phase was applied to an aminopropyl (NH 2 ) cartridge to eluate isoprostanes (Moretti, Collodel, Salvatici, Belmonte, & Signorini, 2019).…”

Section: Total F 2 -Isoprostane Determinationmentioning

confidence: 99%

“…Thus, our observations on seminal cPLA 2 and F 2 -IsoP levels might suggest that the sperm quality may decrease for two reasons: a change in FA content caused by oxidative stress and an altered FA profile during membranes modelling in spermatogenic process.Accordingly, the relation between FA profile of the sperm membrane and cPLA 2 and F 2 -IsoP should be further investigated in different experimental conditions such as animal model or in vitro studies.Interestingly, a reduction in spermatozoa cPLA2 amount, together with an increase of F2-IsoP levels in spermatozoa and seminal plasma, was reported in spermatozoa of a globozoospermic patient(Moretti et al, 2019). These values referred to a limited number of patients because not all showed a sufficient sperm concentration to carry out both TEM analysis, cPLA 2 and F 2 -IsoP determinations.…”

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confidence: 92%

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Cytosolic phospholipase A ₂ and F ₂ isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility

Collodel

Castellini

Iacoponi³

et al. 2019

Andrologia

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Phospholipase A 2 (PLA 2 ) is involved in eicosanoid release, and F 2 -isoprostanes (F 2 -IsoPs), as free radical-generated eicosanoids released by PLA 2 , are indicators of oxidative stress in different human conditions. This study investigated the interplay between cytosolic PLA 2 (cPLA 2 ), F 2 -IsoPs and sperm features in male infertility, when the involvement of oxidative stress has been reported. Semen evaluation was performed following WHO guidelines, sperm ultrastructure was detected by transmission electron microscopy indicating a fertility index, and the percentages of sperm immaturity, apoptosis and necrosis. In sperm cells and seminal plasma, cPLA 2 levels were determined by immunological method, whereas F 2 -IsoPs by mass spectrometry.Sperm concentration, morphology, vitality and fertility index values were significantly lower in infertile groups compared with fertile men. An increase in sperm apoptosis and necrosis (p < .01), apoptosis (p < .01) and immaturity (p < .001) was detected in leucocytospermia, idiopathic infertility and varicocele, respectively. Seminal cPLA 2 showed the highest value in varicocele group (p < .05), whereas seminal F 2 -IsoPs increased in varicocele (p < .001) and leucocytospermia (p < .05) groups. In the whole population, F 2 -IsoP and cPLA 2 levels were positively correlated (p < .05). On the contrary, F 2 -IsoPs and cPLA 2 were not significantly different when investigated in sperm cells. Our data indicate that fatty acid oxidation/metabolism plays a role in different male reproductive pathological conditions. K E Y W O R D Scytosolic PLA 2 , human semen, leucocytospermia, sperm cells, varicocele

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Centriolar defects, centrin 1 alterations, and FISH studies in human spermatozoa of a male partner of a couple that produces aneuploid embryos in natural and artificial fertilization

Moretti

Noto

Guazzo

et al. 2021

J Assist Reprod Genet

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Purpose To study the potential paternal contribution to aneuploidies in the man of a couple who obtained trisomic embryos with natural and assisted fertilization. Methods Semen analysis, immunofluorescence for localization of tubulin and centrin 1, transmission electron microscopy (TEM), and fluorescence in situ hybridization (FISH) analysis for chromosomes 18 and 9 were performed. Sperm of fertile men were used as controls. Results The percentages of sperm motility and normal forms were decreased. The percentages of sperm with tail reduced in dimension, headless tails, coiled tails, and altered head-tail junction were significantly higher (P < 0.01) in the patient than in controls, whereas the percentage of sperm with a normal centrin 1 localization (two spots in the centriolar area) was significantly reduced (P < 0.01) in the patient. Immunofluorescence with anti-tubulin antibody showed that in most of the patient’s sperm connecting pieces (83.00 ± 1.78%), two spots were present, indicating prominent proximal centriole/centriolar adjunct and evident distal centriole, whereas controls’ sperm displayed a single spot, indicating the proximal centriole. The percentage of sperm with two spots was significantly higher (P < 0.01) in the patient than in controls. TEM analysis showed that centriolar adjuncts of the patient’s sperm were significantly longer (721.80 ± 122.26 nm) than in controls’ sperm (310.00 ± 64.11 nm; P < 0.001). The aneuploidy frequencies of the patient’s sperm, detected by FISH analysis, were increased with respect to controls. Conclusion A paternal contribution to sperm aneuploidies cannot be excluded since the patient’s sperm showed altered morphology, immature centriolar adjunct, presence of evident distal centriole, scarce presence of centrin 1, and high aneuploidy frequency.

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New insights into sperm with total globozoospermia: Increased fatty acid oxidation and centrin1 alteration

Cited by 25 publications

References 57 publications

Globozoospermia syndrome: An update

Globozoospermia syndrome: An update

Cytosolic phospholipase A ₂ and F ₂ isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility

Centriolar defects, centrin 1 alterations, and FISH studies in human spermatozoa of a male partner of a couple that produces aneuploid embryos in natural and artificial fertilization

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New insights into sperm with total globozoospermia: Increased fatty acid oxidation and centrin1 alteration

Cited by 25 publications

References 57 publications

Globozoospermia syndrome: An update

Globozoospermia syndrome: An update

Cytosolic phospholipase A 2 and F 2 isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility

Centriolar defects, centrin 1 alterations, and FISH studies in human spermatozoa of a male partner of a couple that produces aneuploid embryos in natural and artificial fertilization

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Cytosolic phospholipase A ₂ and F ₂ isoprostanes are involved in semen quality and human infertility—A study on leucocytospermia, varicocele and idiopathic infertility