New Insights into Peptide–Silver Nanoparticle Interaction: Deciphering the Role of Cysteine and Lysine in the Peptide Sequence

Poblete, Horacio; Agarwal, Anirudh; Thomas, Suma S.; Bohne, Cornelia; Ranjithkumar, R.; Phopase, Jaywant; Comer, Jeffrey; Alarcon, Emilio I.

doi:10.1021/acs.langmuir.5b03601

Cited by 51 publications

(52 citation statements)

References 46 publications

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“…Like AuNPs, AgNPs are small in size, have high surface area-to-volume ratios, and display unique electrical and optical properties resulting in high reactivity and chemical stability [23]. In addition, thiol chemistry can be utilized for direct conjugation to the AgNP, which allows for an easy method of conjugation that has been shown to increase both the stability and activity of the bound AMP [114][115][116]. However, AgNPs generally exhibit high cytotoxicity and are thus not necessarily a great delivery vehicle for improving AMP tolerability.…”

Section: Silver Nanoparticlesmentioning

confidence: 99%

Towards Robust Delivery of Antimicrobial Peptides to Combat Bacterial Resistance

2020

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Antimicrobial peptides (AMPs), otherwise known as host defence peptides (HDPs), are naturally occurring biomolecules expressed by a large array of species across the phylogenetic kingdoms. They have great potential to combat microbial infections by directly killing or inhibiting bacterial activity and/or by modulating the immune response of the host. Due to their multimodal properties, broad spectrum activity, and minimal resistance generation, these peptides have emerged as a promising response to the rapidly concerning problem of multidrug resistance (MDR). However, their therapeutic efficacy is limited by a number of factors, including rapid degradation, systemic toxicity, and low bioavailability. As such, many strategies have been developed to mitigate these limitations, such as peptide modification and delivery vehicle conjugation/encapsulation. Oftentimes, however, particularly in the case of the latter, this can hinder the activity of the parent AMP. Here, we review current delivery strategies used for AMP formulation, focusing on methodologies utilized for targeted infection site release of AMPs. This specificity unites the improved biocompatibility of the delivery vehicle with the unhindered activity of the free AMP, providing a promising means to effectively translate AMP therapy into clinical practice.

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Section: Silver Nanoparticlesmentioning

confidence: 99%

Towards Robust Delivery of Antimicrobial Peptides to Combat Bacterial Resistance

2020

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“…To improve their efficacy and stability in biomedical and other applications, the nanoparticles often need to be coated with non-toxic and non-inflammatory capping agents like collagen, peptides, and biopolymers, preferably without significantly changing their size, shape, and antimicrobial properties [ 3 , 15 , 16 , 17 , 18 , 19 ]. Some findings concerning the action of AgNPs suggest that the bactericidal mode can be influenced by the stability of the particles [ 5 ], and as one example AgNPs conjugated with poly-lysine were less effective when compared to collagen-capped AgNPs due to aggregation.…”

Section: Introductionmentioning

confidence: 99%

Poly-L-arginine Coated Silver Nanoprisms and Their Anti-Bacterial Properties

et al. 2017

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The aim of this study was to test the effect of two different morphologies of silver nanoparticles, spheres, and prisms, on their antibacterial properties when coated with poly-L-arginine (poly-Arg) to enhance the interactions with cells. Silver nanoparticle solutions were characterized by UV–visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta potential, as well as antimicrobial tests. These ultimately showed that a prismatic morphology exhibited stronger antimicrobial effects against Escherichia coli, Pseudomonas aeruginosa and Salmonella enterica. The minimum bactericidal concentration was found to be 0.65 μg/mL in the case of a prismatic AgNP-poly-Arg-PVP (silver nanoparticle-poly-L-arginine-polyvinylpyrrolidone) nanocomposite. The anticancer cell activity of the silver nanoparticles was also studied, where the maximum effect against a HeLa cell line was 80% mortality with a prismatic AgNP-poly-Arg-PVP nanocomposite at a concentration of 11 μg/mL. The antimicrobial activity of these silver nanocomposites demonstrates the potential of such coated silver nanoparticles in the area of nano-medicine.

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“…Though the exact mechanism of AgNP binding to Msr1 receptor is not known, we speculate that it might be unspecific binding to thiol (-SH) groups. AgNP have a strong affinity for cysteine tiol (-SH) groups of proteins [ 46 ] that are abundant in Msr1 scavenger receptor [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells

et al. 2020

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Microglial cells clear the brain of pathogens and harmful debris, including amyloid-β (Aβ) deposits that are formed during Alzheimer’s disease (AD). We studied the expression of Msr1, Ager and Cd36 receptors involved in Aβ uptake and expression of Cd33 protein, which is considered a risk factor in AD. The effect of silver nanoparticles (AgNPs) and cadmium telluride quantum dots (CdTeQDs) on the expression of the above receptors and Aβ uptake by microglial cells was investigated. Absorption of Aβ and NPs was confirmed by confocal microscopy. AgNPs, but not CdTeQDs, caused a decrease in Aβ accumulation. By using a specific inhibitor—polyinosinic acid—we demonstrated that Aβ and AgNPs compete for scavenger receptors. Real-time PCR showed up-regulation of Cd33 and Cd36 gene expression after treatment with CdTeQDs for 24 h. Analysis of the abundance of the receptors on the cell surface revealed that AgNP treatment significantly reduced the presence of Msr1, Cd33, Ager and Cd36 receptors (6 and 24 h), whereas CdTeQDs increased the levels of Msr1 and Cd36 (24 h). To summarize, we showed that AgNP uptake competes with Aβ uptake by microglial cells and consequently can impair the removal of the aggregates. In turn, CdTeQD treatment led to the accumulation of proinflammatory Cd36 protein on the cell surface.

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New Insights into Peptide–Silver Nanoparticle Interaction: Deciphering the Role of Cysteine and Lysine in the Peptide Sequence

Cited by 51 publications

References 46 publications

Towards Robust Delivery of Antimicrobial Peptides to Combat Bacterial Resistance

Towards Robust Delivery of Antimicrobial Peptides to Combat Bacterial Resistance

Poly-L-arginine Coated Silver Nanoprisms and Their Anti-Bacterial Properties

The Impact of Ag Nanoparticles and CdTe Quantum Dots on Expression and Function of Receptors Involved in Amyloid-β Uptake by BV-2 Microglial Cells

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