New insights into galactose metabolism by Schizosaccharomyces pombe: Isolation and characterization of a galactose-assimilating mutant

Matsuzawa, T.; Fujita, Yuka; Tanaka, Naotaka; Tohda, Hideki; Itadani, Akiko; Takegawa, Kaoru

doi:10.1016/j.jbiosc.2010.10.007

Cited by 23 publications

(17 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditional tests indicate that S. pombe cannot grow on galactose (2), but its genome contains a set of GAL pathway genes predicted to be functional. Indeed, mutants of S. pombe have been isolated that grow on galactose and constitutively express their GAL pathways (33), suggesting that S. pombe may respond to a different induction signal. Similarly, S. cerevisiae, although not classified as a xylose-using yeast (2), has been shown to grow on xylose when endogenous genes are overexpressed (34).…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics of biotechnologically important yeasts

Riley

Haridas

Wolfe

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

303

394

View full text Add to dashboard Cite

Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Our well-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as L-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation.genomics | bioenergy | biotechnological yeasts | genetic code | microbiology Y easts are fungi that reproduce asexually by budding or fission and sexually without multicellular fruiting bodies (1, 2). Their unicellular, largely free-living lifestyle has evolved several times (3). Despite morphological similarities, yeasts constitute over 1,500 known species that inhabit many specialized environmental niches and associations, including virtually all varieties of fruits and flowers, plant surfaces and exudates, insects and other invertebrates, birds, mammals, and highly diverse soils (4). Biochemical and genomic studies of the model yeast Saccharomyces cerevisiaeessential for making bread, beer, and wine-have established much of our understanding of eukaryotic biology. However, in many ways, S. cerevisiae is an oddity among the yeasts, and many important biotechnological applications and highly divergent physiological capabilities of lesser-known yeast species have not been fully exploited (5). Various species can grow on methanol or n-alkanes as sole carbon and energy sources, overproduce vitamins and lipids, thrive under acidic conditions, and ferment unconventional carbon sources. Many features of yeasts make them ideal platforms for biotechnological processes. Their thick cell walls help them survive osmotic shock, and in contrast to bacteria, they are resistant to viruses. Their unicellular form is easy to cultivate, scale up, and harvest. The objective of this study was, therefore, to put yeasts with diverse biotechnological applications in a phylogenomic context and relate their physiologies to genomic SignificanceThe highly diverse Ascomycete yeasts have enormous biotechnological potential. Collectively, these yeasts convert a broad range of substrates into useful compounds, such as ethanol, lipids, and vitamins, and can grow in extremes of temperature, salinity, and pH. We compared 29 yeast genome...

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative genomics of biotechnologically important yeasts

Riley

Haridas

Wolfe

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

303

394

View full text Add to dashboard Cite

show abstract

“…A novel mutagenesis technique using error-prone DNA polymerase δ ( pol δ ), based on the disparity mutagenesis model of evolution [3] has been successfully employed to generate novel microorganism strains with desired traits [4–11]. In the disparity model, mutations occur preferentially on the lagging strand, due to the more complex, discontinuous DNA replication that takes place there.…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Profiling of a Novel Mutagenesis Technique Using Proofreading-Deficient DNA Polymerase δ

Shiwa

Fukushima-Tanaka²,

Kasahara³

et al. 2012

International Journal of Evolutionary Biology

View full text Add to dashboard Cite

A novel mutagenesis technique using error-prone DNA polymerase δ (polδ), the disparity mutagenesis model of evolution, has been successfully employed to generate novel microorganism strains with desired traits. However, little else is known about the spectra of mutagenic effects caused by disparity mutagenesis. We evaluated and compared the performance of the polδ MKII mutator, which expresses the proofreading-deficient and low-fidelity polδ, in Saccharomyces cerevisiae haploid strain with that of the commonly used chemical mutagen ethyl methanesulfonate (EMS). This mutator strain possesses exogenous mutant polδ supplied from a plasmid, tthereby leaving the genomic one intact. We measured the mutation rate achieved by each mutagen and performed high-throughput next generation sequencing to analyze the genome-wide mutation spectra produced by the 2 mutagenesis methods. The mutation frequency of the mutator was approximately 7 times higher than that of EMS. Our analysis confirmed the strong G/C to A/T transition bias of EMS, whereas we found that the mutator mainly produces transversions, giving rise to more diverse amino acid substitution patterns. Our present study demonstrated that the polδ MKII mutator is a useful and efficient method for rapid strain improvement based on in vivo mutagenesis.

show abstract

“…1C, lane 4). Neither sorbitol nor galactose, an isomer of glucose that cannot be used as a carbon source (20), suppressed Hmg1 phosphorylation (Fig. 1C, lanes 3 and 5).…”

Section: Resultsmentioning

confidence: 99%