Glucose Controls Phosphoregulation of Hydroxymethylglutaryl Coenzyme A Reductase through the Protein Phosphatase 2A-related Phosphatase Protein, Ppe1, and Insig in Fission Yeast

Burg, John S.; Espenshade, Peter J.

doi:10.1074/jbc.m111.233452

Cited by 9 publications

(15 citation statements)

References 30 publications

(42 reference statements)

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“…6)[31, 67]. Hmg1 phosphorylation in minimal medium and osmotic stress requires the stress-responsive mitogen-activated protein kinase (MAPK) Sty1 and its upstream activators Wis4 and Wis1 [31, 68, 69].…”

Section: Regulation Of Schizosaccharomyces Pombe Hmg-coa Reductasementioning

confidence: 99%

“…6). Low glucose strongly induces phosphorylation of Hmg1 S1024 and T1028, decreasing Hmg1 activity 3-fold [67]. Low glucose-stimulated Hmg1 phosphorylation requires the PP2A-related phosphatase Ppe1 and its negative regulator Sds23 [67, 70].…”

Section: Regulation Of Schizosaccharomyces Pombe Hmg-coa Reductasementioning

confidence: 99%

“…Low glucose strongly induces phosphorylation of Hmg1 S1024 and T1028, decreasing Hmg1 activity 3-fold [67]. Low glucose-stimulated Hmg1 phosphorylation requires the PP2A-related phosphatase Ppe1 and its negative regulator Sds23 [67, 70]. In cells lacking the Ppe1 phosphatase, Hmg1 is constitutively phosphorylated at S1024 and shows no further increase in S1024 phosphorylation in low glucose [67].…”

Section: Regulation Of Schizosaccharomyces Pombe Hmg-coa Reductasementioning

confidence: 99%

“…Low glucose-stimulated Hmg1 phosphorylation requires the PP2A-related phosphatase Ppe1 and its negative regulator Sds23 [67, 70]. In cells lacking the Ppe1 phosphatase, Hmg1 is constitutively phosphorylated at S1024 and shows no further increase in S1024 phosphorylation in low glucose [67]. The opposite is true of cells lacking sds23 + ; sds23Δ cells show no Hmg1 phosphorylation, even in low glucose.…”

Section: Regulation Of Schizosaccharomyces Pombe Hmg-coa Reductasementioning

confidence: 99%

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Regulation of HMG-CoA reductase in mammals and yeast

Burg

Espenshade

2011

Progress in Lipid Research

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241

199

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HMG-CoA reductase (HMGR), a highly conserved, membrane-bound enzyme, catalyzes a rate-limiting step in sterol and isoprenoid biosynthesis and is the primary target of hypocholesterolemic drug therapy. HMGR activity is tightly regulated to ensure maintenance of lipid homeostasis, disruption of which is a major cause of human morbidity and mortality. HMGR regulation takes place at the levels of transcription, translation, post-translational modification and degradation. In this review, we discuss regulation of mammalian, Saccharomyces cerevisiae and Schizosaccharomyces pombe HMGR and highlight recent advances in the field. We find that the general features of HMGR regulation, including a requirement for the HMGR-binding protein Insig, are remarkably conserved between mammals and ascomycetous fungi, including S. cerevisiae and S. pombe. However the specific details by which this regulation occurs differ in surprising ways, revealing the broad evolutionary themes underlying both HMGR regulation and Insig function.

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Section: Regulation Of Schizosaccharomyces Pombe Hmg-coa Reductasementioning

confidence: 99%

Section: Regulation Of Schizosaccharomyces Pombe Hmg-coa Reductasementioning

confidence: 99%

Section: Regulation Of Schizosaccharomyces Pombe Hmg-coa Reductasementioning

confidence: 99%

Section: Regulation Of Schizosaccharomyces Pombe Hmg-coa Reductasementioning

confidence: 99%

See 2 more Smart Citations

Regulation of HMG-CoA reductase in mammals and yeast

Burg

Espenshade

2011

Progress in Lipid Research

Self Cite

241

199

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show abstract

“…Ins1 binding to Hmg1 stimulates its phosphorylation through a stress responsive MAP kinase Sty1 inhibiting enzyme activity. Hmg1 activity is also suppressed by glucose starvation through by the inhibitory effect of Sds23 on the phosphatase Ppe1 [59]. …”

Section: Post-translational Regulation Of Sterol Synthesismentioning

confidence: 99%

Regulation of lipid metabolism: a tale of two yeasts

Raychaudhuri

Young

Espenshade

et al. 2012

Current Opinion in Cell Biology

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Eukaryotic cells synthesize multiple classes of lipids by distinct metabolic pathways in order to generate membranes with optimal physical and chemical properties. As a result, complex regulatory networks are required in all organisms to maintain lipid and membrane homeostasis as well as to rapidly and efficiently respond to cellular stress. The unicellular nature of yeast makes it particularly vulnerable to environmental stress and yeast has evolved elaborate signaling pathways to maintain lipid homeostasis. In this article we highlight the recent advances that have been made using the budding and fission yeasts and we discuss potential roles for the unfolded protein response (UPR) and the SREBP-Scap pathways in coordinate regulation of multiple lipid classes.

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Metabolism of Storage Lipids and the Role of Lipid Droplets in the Yeast Schizosaccharomyces pombe

Hapala

Griač

Holic̆

2020

Lipids

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Storage lipids, triacylglycerols (TAG), and steryl esters (SE), are predominant constituents of lipid droplets (LD) in fungi. In several yeast species, metabolism of TAG and SE is linked to various cellular processes, including cell division, sporulation, apoptosis, response to stress, and lipotoxicity. In addition, TAG are an important source for the generation of value-added lipids for industrial and biomedical applications. The fission yeast Schizosaccharomyces pombe is a widely used unicellular eukaryotic model organism. It is a powerful tractable system used to study various aspects of eukaryotic cellular and molecular biology. However, the knowledge of S. pombe neutral lipids metabolism is quite limited. In this review, we summarize and discuss the current knowledge of the homeostasis of storage lipids and of the role of LD in the fission yeast S. pombe with the aim to stimulate research of lipid metabolism and its connection with other essential cellular processes. We also discuss the advantages and disadvantages of fission yeast in lipid biotechnology and recent achievements in the use of S. pombe in the biotechnological production of valuable lipid compounds.

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Glucose Controls Phosphoregulation of Hydroxymethylglutaryl Coenzyme A Reductase through the Protein Phosphatase 2A-related Phosphatase Protein, Ppe1, and Insig in Fission Yeast

Abstract: HMG-

Cited by 9 publications

References 30 publications

Regulation of HMG-CoA reductase in mammals and yeast

Regulation of HMG-CoA reductase in mammals and yeast

Regulation of lipid metabolism: a tale of two yeasts

Metabolism of Storage Lipids and the Role of Lipid Droplets in the Yeast Schizosaccharomyces pombe

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