2018
DOI: 10.1016/j.meegid.2018.10.001
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New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycle of the Argentinean Chaco

Abstract: Trypanosoma cruzi, the etiological agent of Chagas disease, was initially classified into 6 Discrete Typing Units (DTUs). The hybrid DTUs TcV and TcVI are the most frequent in domestic transmission cycles throughout the Southern Cone countries of South America. Here, we genotyped parasite isolates from human residents in Pampa del Indio municipality, Chaco, to further characterize the structure of T. cruzi populations, and to assess the degree of overlapping between the domestic and sylvatic transmission cycle… Show more

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Cited by 16 publications
(16 citation statements)
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“…To our knowledge, there are few reports of TSSApep-II/V/VI as an antigen for assay with paired samples from chronic patients. Of pre-treatment samples screened by Chagas Sero K-SeT RDT for TSSApep-V/V/VI infection, 91.5% were positive, confirming that these are the predominant infecting lineages in the region, as previously reported [ 30 , 63 ]. Among the patients who completed chemotherapy, a proportion of Chagas Sero K-SeT RDT positives (43.6%) showed IgG decline post-treatment, by visual assessment of band intensity.…”
Section: Discussionsupporting
confidence: 81%
“…To our knowledge, there are few reports of TSSApep-II/V/VI as an antigen for assay with paired samples from chronic patients. Of pre-treatment samples screened by Chagas Sero K-SeT RDT for TSSApep-V/V/VI infection, 91.5% were positive, confirming that these are the predominant infecting lineages in the region, as previously reported [ 30 , 63 ]. Among the patients who completed chemotherapy, a proportion of Chagas Sero K-SeT RDT positives (43.6%) showed IgG decline post-treatment, by visual assessment of band intensity.…”
Section: Discussionsupporting
confidence: 81%
“…Additionally, in accordance with the UPGMA results of our study, Macchiaverna and collaborators showed that the sequencing data of the T. cruzi TcMK (mevalonate kinase) gene in clinical samples from chronic patients in Argentina, revealed a low rate of genetic variability within the DTU TcV, and showing apparently two robust subgroups of isolates (39).…”
Section: Trypanosomasupporting
confidence: 89%
“…Furthermore, none of the 30 seronegative samples was positive by this RDT, indicating that the RDT has greater sensitivity, as might be expected because the RDTs employ higher serum concentrations. Using the observed sensitivity and specificity we estimated that the prevalence of infection with TcII/V/VI in the seropositive human population of Pampa del Indio is 88.2% (95% CI: 76.4–99.0%), which provides further support for the prevalence of hybrid lineages in infected humans from the Chaco, as indicated by time-consuming artificial xenodiagnosis, in vitro culture, parasite isolation and PCR-based lineage identification [35].…”
Section: Discussionmentioning
confidence: 99%
“…Trypanosoma cruzi lineage was determined by PCR of the genomic targets spliced-leader (SL) DNA, 24Sα ribosomal RNA genes and A10 from T. cruzi isolates [32, 33]. For humans only, a second PCR-based protocol targeting two nuclear genes (TcSC5D and TcMK) [34] was also employed [35] to allow for classification of lineages TcI-TcVI as well as TcBat and TcV/VI [34].…”
Section: Methodsmentioning
confidence: 99%