New horizons in spine research: Disc biology, tissue engineering, biomechanics, translational, and clinical research

Dahia, Chitra Lekha; Iatridis, James C.; Risbud, Makarand V.

doi:10.1002/jsp2.1032

Cited by 7 publications

(6 citation statements)

References 11 publications

(17 reference statements)

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“…Animal models and organ culture models are important preclinical tools for biomedical research [ 15 , 16 ]. Ideally, the most appropriate preclinical model for a specific disease is to artificially develop a condition that replicates the human condition.…”

Section: Introductionmentioning

confidence: 99%

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One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

Zhou

Cui

et al. 2021

Journal of Orthopaedic Translation

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Section: Introductionmentioning

confidence: 99%

“…Notably, the risk of disc degeneration is increased in manual laborers such as carpenters and drivers of machinery [ 24 ]. Excessive mechanical loading or acute trauma to IVDs is thought to contribute to the degeneration and pain [ 15 , [25] , [26] , [27] ]. Ghanem et al.…”

Section: Introductionmentioning

confidence: 99%

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

Zhou

Cui

et al. 2021

Journal of Orthopaedic Translation

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“…[29][30][31] In vivo animal injury models have challenges in implementing controlled cellular stimulation and quantitatively assessing cellular responses. [5,[32][33][34] On the other hand, in vitro models, such as monolayer and 3D cell culture, do not reflect the complexity of in vivo diseases due to lack of proper cell-cell and cell-matrix interactions. [35,36] A number of disc organ culture systems have been established, such as well-established bovine disc organ culture models.…”

Section: Design Of Intervertebral Disc-on-a-chip Mf System For Mouse ...mentioning

confidence: 99%

Intervertebral Disc‐on‐a‐Chip^MF: A New Model for Mouse Disc Culture via Integrating Mechanical Loading and Dynamic Media Flow

Xie,

Xing,

Xiao

et al. 2023

Adv Materials Technologies

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This study aims to develop an ex vivo organ‐on‐a‐chip model, intervertebral Disc‐on‐a‐ChipMF, to investigate integrated effects of mechanical loading and nutrition on disc health. The system consists of a detachable multilayer microfluidic chip, a Computer‐Arduino‐based control system, and a mechanical loading unit, which are optimized for accurate axial force measurement and the maintenance of a 21‐day ex vivo disc culture. To ensure accuracy of axial force, the axial mechanical loading regimen is optimized, using the Computer‐Arduino‐based system and low‐profile force sensors (LPFS) to control the mechanical loading unit, and the force distribution on the disc surface is modeled by computational simulation. A 21‐day ex vivo disc culture is demonstrated using the Disc‐on‐a‐ChipMF system, with optimized mechanical loading (0.02 MPa at 1Hz, 1.5 hr day−1) and flow rate (1 µL min−1). The structural integrity, collagen breakdown, catabolic enzyme activities, and disc cell and collagen alignment reveal that the on‐chip cultured discs exhibit a preferred disc health similar to that of native discs for up to 21 days, while discs in a static culture show degenerative changes. The mouse Disc‐on‐a‐ChipMF system mimics in vivo disc microenvironment and provides a valuable platform for studying the effects of various factors on disc health and degeneration and testing new therapies.

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“…Understanding the mechanisms of IVD degeneration to stop or reverse this process or re-establishing function through therapies is a challenge at the intersection of biomaterials, biomechanics, clinical practice, and biology [ 132 , 133 , 134 ]. Despite intense research interest in this area, lack of suitable collaborations has been a severe limitation towards the creation of effective therapeutic devices to regenerate the AF.…”

Section: Future Outlookmentioning

confidence: 99%

Advanced Strategies for the Regeneration of Lumbar Disc Annulus Fibrosus

Tavakoli

Diwan

Tipper

2020

IJMS

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Damage to the annulus fibrosus (AF), the outer region of the intervertebral disc (IVD), results in an undesirable condition that may accelerate IVD degeneration causing low back pain. Despite intense research interest, attempts to regenerate the IVD have failed so far and no effective strategy has translated into a successful clinical outcome. Of particular significance, the failure of strategies to repair the AF has been a major drawback in the regeneration of IVD and nucleus replacement. It is unlikely to secure regenerative mediators (cells, genes, and biomolecules) and artificial nucleus materials after injection with an unsealed AF, as IVD is exposed to significant load and large deformation during daily activities. The AF defects strongly change the mechanical properties of the IVD and activate catabolic routes that are responsible for accelerating IVD degeneration. Therefore, there is a strong need to develop effective therapeutic strategies to prevent or reconstruct AF damage to support operational IVD regenerative strategies and nucleus replacement. By the way of this review, repair and regenerative strategies for AF reconstruction, their current status, challenges ahead, and future outlooks were discussed.

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New horizons in spine research: Disc biology, tissue engineering, biomechanics, translational, and clinical research

Cited by 7 publications

References 11 publications

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

Intervertebral Disc‐on‐a‐Chip^MF: A New Model for Mouse Disc Culture via Integrating Mechanical Loading and Dynamic Media Flow

Advanced Strategies for the Regeneration of Lumbar Disc Annulus Fibrosus

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New horizons in spine research: Disc biology, tissue engineering, biomechanics, translational, and clinical research

Cited by 7 publications

References 11 publications

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

One strike loading organ culture model to investigate the post-traumatic disc degenerative condition

Intervertebral Disc‐on‐a‐ChipMF: A New Model for Mouse Disc Culture via Integrating Mechanical Loading and Dynamic Media Flow

Advanced Strategies for the Regeneration of Lumbar Disc Annulus Fibrosus

Contact Info

Product

Resources

About

Intervertebral Disc‐on‐a‐Chip^MF: A New Model for Mouse Disc Culture via Integrating Mechanical Loading and Dynamic Media Flow