New genetic markers to identify European resistant abalone to vibriosis revealed by high-resolution melting analysis, a sensitive and fast approach

Abstract

International audienceIncreasing temperature of seawater is often associated with increased exposure incidence of disease in field and in aquaculture populations. Numerous episodic mass mortalities of the abalone Haliotis tuberculata have been observed along the northern Brittany coast of France caused by a complex interaction between the host, pathogen and environmental factors. Here, we evaluated the potential of high-resolution melting (HRM) analysis for mutation genotyping and development of genetic markers for resistance to vibriosis in the gastropod species H. tuberculata. Small amplicon assays were developed and revealed genetic polymorphism between surviving and susceptible abalone obtained after two successive infections of aquaculture families in controlled conditions. Together with specific COI haplotypes, we identified particular genotypes in nascent polypeptide-associated complex subunit alpha and ferritin genes linked to the susceptibility or resistance of abalone to vibriosis. Selection of genitors based on these genes may increase the proportion in offspring of resistant individuals of more than 76 %. Finally, HRM assays constitute a very efficient genotyping tool to validate the genetic markers on a representative number of individuals of wild populations and thus identify future resistant genitors for aquaculture or conservation purpose

Signatures of selection for bonamiosis resistance in European flat oyster (Ostrea edulis): New genomic tools for breeding programs and management of natural resources

“…Accordingly, detection of quantitative trait loci (QTL) and markers associated with resistance to bonamiosis could greatly accelerate the selection of breeders making the process much more efficient. Such resistance markers have been successfully found for some diseases in mollusks (He, Yu, Bao, Zhang, & Guo, 2012;Meistertzheim et al, 2014;Nie, Yue, & Liu, 2015;Nikapitiya et al, 2014;Normand et al, 2014;Raftos, Kuchel, Aladaile, & Butt, 2014;Schmitt, Santini, Vergnes, Degremont, & de Lorgeril, 2013), and particularly, several QTL for resistance to bonamiosis have been identified in flat oyster (Harrang et al, 2015;Lallias et al, 2009). These studies typically used low-medium density genetic maps and a small number of families and were able to identify some QTLs explaining up to 17% of the phenotypic variance in the trait (Harrang et al, 2015;Lallias et al, 2009).…”

Transcriptome characterization of the common cockle (Cerastoderma edule) after exposure to aMarteilia cochilliaoutbreak

“…Understanding the molecular basis of the differences responsible for being susceptible or resistant to a particular disease and identifying molecular markers of resistance can help to fight against mollusc diseases. Recently, research effort has been focused on the identification of molecular markers of disease resistance, through transcriptomics (He et al, 2012; Meistertzheim et al, 2014; Nikapitiya et al, 2014; Nie et al, 2015; Wang et al, 2016; Gutierrez et al, 2018, 2020; La Peyre et al, 2019; De Lorgeril et al, 2020; Farhat et al, 2020; Proestou and Sullivan, 2020; Hasanuzzaman et al, 2020), proteomics (Simonian et al, 2009; Fernández-Boo et al, 2016; de la Ballina et al, 2018; Vaibhav et al, 2018; Smits et al, 2020; Leprêtre et al, 2021) and population genomics approaches (Vera et al, 2019; Sambade et al, 2022). This information could be eventually used for marker-assisted selection (MAS) programs to increase resilience against pathogens.…”

Bacteriophage therapy for the control of Vibrio harveyi in greenlip abalone (Haliotis laevigata)

“…This disease is caused by a range of Vibrio species, which can lead to mass mortality in a relatively short time (Jiang et al 2013). Vibriosis-associated mass mortalities in abalone have been reported in France (Travers et al 2008b, Pichon et al 2013, Cardinaud et al 2014a, Meistertzheim et al 2014, Australia (Handlinger et al 2005, Travers et al 2009b, Dang et al 2012, and Japan (Travers et al 2009b). Abalone vibriosis usually occurs when animals are immunosuppressed due to conditions such as elevated water temperature or spawning (Pichon et al 2013, Cardinaud et al 2014a.…”

“…Previous studies showed that individual abalone undergoing gonad development are more susceptible to vibriosis, while immature abalone are more resistant to natural infection (Travers et al 2009a, Meistertzheim et al 2014. Since controlling water temperature on farms during such elevated temperatures is impractical for long periods of time, there is an industry need for effective vibriosis treatments for mature abalone.…”